类风湿关节炎患者外周血T细胞PD1表达的分析和意义

发布时间：2018-11-21 11:27

【摘要】：目的探讨程序性死亡分子1(PD1)在类风湿关节炎(RA)患者外周血CD4~+T和CD8~+T细胞上的表达及临床意义。方法应用流式细胞仪检测66例RA患者和30例健康对照者外周血CD4~+T和CD8~+T细胞上PD1表达(平均荧光强度和百分率),比较RA组和健康对照组之间CD4~+T和CD8~+T细胞上PD1表达水平,分析其与RA实验室检查指标的相关性。结果 (1)RA患者CD4~+T、CD8~+T细胞PD1表达的平均荧光强度(MFI)显著高于对照组,差异均有统计学意义(P0.05);RA患者CD4~+PD1~+T细胞百分率显著高于对照组,差异有统计学意义(P0.05)。(2)RA患者外周血CD4~+T、CD8~+T细胞PD1表达的MFI与DAS28评分呈正相关性(r=0.49,P0.000 1;r_s=0.40,P=0.0008)。(3)RA患者外周血CD4~+T细胞PD1表达的MFI与血沉(ESR)、C-反应蛋白(CRP)呈正相关性(r=0.45,P0.000 1;r=0.39,P=0.001);RA患者外周血CD8~+T细胞PD1表达的MFI与CRP呈正相关性(r_s=0.40,P=0.0009);RA患者外周血CD4~+PD1~+T细胞百分率与CRP呈正相关性(r=0.24,P=0.048)。(4)RA患者外周血CD8~+T细胞PD1表达的MFI与类风湿因子(RF)呈正相关性(r_s=0.32,P=0.009);RA患者外周血CD4~+PD1~+T细胞百分率与RF呈正相关性(r=0.27,P=0.030)。结论 RA患者外周血CD4~+T和CD8~+T细胞PD1表达异常,与疾病的活动性及抗体产生有关。
[Abstract]:Objective to investigate the expression and clinical significance of programmed death molecule 1 (PD1) on CD4~ T and CD8~ T cells in peripheral blood of (RA) patients with rheumatoid arthritis. Methods the expression of PD1 on peripheral blood CD4~ T and CD8~ T cells (mean fluorescence intensity and percentage) was detected by flow cytometry in 66 patients with RA and 30 healthy controls. The PD1 expression on CD4~ T and CD8~ T cells was compared between RA group and healthy control group. To analyze the correlation between it and RA laboratory examination index. Results (1) the average fluorescence intensity (MFI) of PD1 expression in CD4~ T cell CD8 ~ T cells in RA patients was significantly higher than that in the control group (P0.05). The percentage of CD4~ PD1~ T cells in patients with RA was significantly higher than that in the control group (P0.05). (2). The MFI expression of CD8T cells in peripheral blood of RA patients was positively correlated with DAS28 score (r = 0.49, P 0.000 1). There was a positive correlation between the PD1 expression of CD4~ T cells in peripheral blood and (ESR), C-reactive protein (CRP) in peripheral blood of patients with RA. There was a positive correlation between the PD1 expression of CD8~ T cells and CRP in peripheral blood of RA patients. There was a positive correlation between the percentage of CD4~ PD1~ T cells in peripheral blood of RA patients and CRP (r = 0.24 P0. 048). (4). The PD1 expression of CD8~ T cells in RA patients was positively correlated with rheumatoid factor (RF). The percentage of CD4~ PD1~ T cells in peripheral blood of patients with RA was positively correlated with RF (r = 0.27, P = 0.030). Conclusion the abnormal PD1 expression of CD4~ T and CD8~ T cells in peripheral blood of patients with RA is related to the activity of the disease and the production of antibodies.
【作者单位】：南昌大学第一附属医院检验科;南昌大学医学院公共卫生学院;
【基金】：国家自然科学基金(编号:81360459) 江西省青年科学基金(编号:20151BAB215031)
【分类号】：R593.22

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