双酚A对高脂饲料小鼠脂肪组织炎症及Th17细胞的影响
发布时间：2018-11-26 10:19
【摘要】：目的探讨双酚A(BPA)暴露对高脂饲料小鼠脂肪组织炎症及Th17细胞的影响。方法高脂饲料(HFD)喂养的4周龄雄性C57BL/6小鼠,通过饮水摄入10、100和1000 nmol/L BPA,并设立正常饲料(ND)和HFD对照组。每周记录小鼠体重,BPA暴露8周和16周后处死小鼠,取附睾脂肪垫,HE染色观察脂肪组织病理学改变,酶联免疫吸附实验(EILSA)检测脂肪组织中炎症因子TNF-α的含量,流式细胞术(FCM)检测脂肪组织中Th17细胞比例,免疫组化(IHC)检测脂肪组织中IL-17的表达。结果 8周和16周时,与ND对照组相比,HFD对照组和HFD饲料BPA暴露组小鼠体重明显增加,附睾脂肪组织炎性细胞浸润增多,TNF-α含量上升,Th17细胞比例增加,IL-17表达上调;与HFD对照组相比,BPA暴露组小鼠附睾脂肪组织炎性细胞浸润增多,TNF-α含量上升,Th17细胞比例增加,IL-17表达上调,且具有剂量-效应关系;与8周时相比,16周时变化更明显。结论 BPA暴露可促进高脂饲料小鼠脂肪组织炎症、Th17细胞比例增加和IL-17表达上调;而Th17细胞可在BPA促进脂肪组织炎症中发挥作用。
[Abstract]:Objective to investigate the effects of bisphenol A (BPA) exposure on adipose tissue inflammation and Th17 cells in mice fed with high fat diet. Methods four week-old male C57BL/6 mice fed with high fat diet (HFD) were fed with drinking water for 10100 nmol/L BPA, and 1000 nmol/L BPA, respectively, and normal diet (ND) and HFD control group were set up. The weight of mice was recorded every week. The mice were killed after BPA exposure for 8 weeks and 16 weeks. The epididymal fat pad was taken, the pathological changes of adipose tissue were observed by HE staining, and the content of inflammatory factor TNF- 伪 in adipose tissue was detected by (EILSA) by enzyme-linked immunosorbent assay (Elisa). The percentage of Th17 cells in adipose tissue was detected by flow cytometry (FCM) and the expression of IL-17 in adipose tissue was detected by immunohistochemical (IHC). Results at 8 and 16 weeks, compared with ND control group, HFD control group and HFD feed BPA exposure group significantly increased their body weight, infiltration of inflammatory cells in epididymal adipose tissue, increase of TNF- 伪 content, increase of Th17 cell proportion and up-regulation of IL-17 expression. Compared with the control group of HFD, the inflammatory cell infiltration of epididymal adipose tissue increased, the content of TNF- 伪 increased, the proportion of Th17 cells increased and the expression of IL-17 increased in BPA exposed group, which had a dose-effect relationship. The changes were more obvious at 16 weeks than at 8 weeks. Conclusion BPA exposure can promote the inflammation of adipose tissue, increase the proportion of Th17 cells and up-regulate the expression of IL-17 in high-fat diet mice, while Th17 cells can play a role in the promotion of adipose tissue inflammation by BPA.
【作者单位】： 安徽医科大学公共卫生学院;
【基金】：国家自然科学基金(No.81473015) 安徽省自然科学基金(No.1508085MH161)
【分类号】：R589.2
[Abstract]:Objective to investigate the effects of bisphenol A (BPA) exposure on adipose tissue inflammation and Th17 cells in mice fed with high fat diet. Methods four week-old male C57BL/6 mice fed with high fat diet (HFD) were fed with drinking water for 10100 nmol/L BPA, and 1000 nmol/L BPA, respectively, and normal diet (ND) and HFD control group were set up. The weight of mice was recorded every week. The mice were killed after BPA exposure for 8 weeks and 16 weeks. The epididymal fat pad was taken, the pathological changes of adipose tissue were observed by HE staining, and the content of inflammatory factor TNF- 伪 in adipose tissue was detected by (EILSA) by enzyme-linked immunosorbent assay (Elisa). The percentage of Th17 cells in adipose tissue was detected by flow cytometry (FCM) and the expression of IL-17 in adipose tissue was detected by immunohistochemical (IHC). Results at 8 and 16 weeks, compared with ND control group, HFD control group and HFD feed BPA exposure group significantly increased their body weight, infiltration of inflammatory cells in epididymal adipose tissue, increase of TNF- 伪 content, increase of Th17 cell proportion and up-regulation of IL-17 expression. Compared with the control group of HFD, the inflammatory cell infiltration of epididymal adipose tissue increased, the content of TNF- 伪 increased, the proportion of Th17 cells increased and the expression of IL-17 increased in BPA exposed group, which had a dose-effect relationship. The changes were more obvious at 16 weeks than at 8 weeks. Conclusion BPA exposure can promote the inflammation of adipose tissue, increase the proportion of Th17 cells and up-regulate the expression of IL-17 in high-fat diet mice, while Th17 cells can play a role in the promotion of adipose tissue inflammation by BPA.
【作者单位】： 安徽医科大学公共卫生学院;
【基金】：国家自然科学基金(No.81473015) 安徽省自然科学基金(No.1508085MH161)
【分类号】：R589.2
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