燃煤型氟中毒对大鼠骺板软骨组织形态学的影响
发布时间：2018-11-26 18:09
【摘要】：目的模拟病区人群复制燃煤型氟中毒大鼠模型,动态观察大鼠不同时期骺板软骨组织形态的改变。方法 150只SD大鼠按体重随机分为对照组、中氟组、高氟组、高氟伴低蛋白低钙组、高氟伴高铝组,每组30只(雌雄各半),分别于第60 d、第120 d、第180 d处死,取大鼠左膝关节,观察大鼠骺板软骨组织病理学改变。结果各染氟组与对照组相比,骺板边缘不齐,软骨细胞钙化延迟,增殖层和肥大层细胞层数增多;高氟伴低蛋白低钙组细胞柱排列紊乱,随染氟时间延长,出现大量坏死区;高氟伴高铝组增殖层软骨偶尔可见结节样增生;对于同一实验组不同时期比较,随着大鼠饲养时间延长,骺板软骨组织的增殖层细胞层数均逐渐降低(P0.05);不同时期3批大鼠各染氟组骺板增殖层、肥大层的细胞层数均多于对照组,且随着染氟剂量增高细胞层数逐渐增加(P0.05);高氟伴低蛋白低钙组、高氟伴高铝组骺板增殖层、肥大层细胞层数较高氟组均降低。结论燃煤型氟中毒大鼠出现了骺板软骨组织的损伤;肥大层、增殖层细胞层数增多;高氟伴低蛋白低钙组大鼠骺板组织出现大量坏死区;高氟伴高铝组大鼠骺板组织增厚主要表现为结节性增生。
[Abstract]:Objective to simulate the rat model of coal-burning fluorosis and observe the morphological changes of epiphyseal plate cartilage in different periods. Methods one hundred and fifty SD rats were randomly divided into control group, middle fluoride group, high fluoride with low protein and low calcium group, and high fluoride and high aluminum group with 30 rats in each group (half male and female). The rats were killed on the 60th, 120th and 180th day, respectively. The histopathological changes of the rat epiphyseal plate cartilage were observed by taking the left knee joint. Results compared with the control group, the epiphyseal plate margin was irregular, the calcification of chondrocytes was delayed, the number of cell layers in proliferative layer and hypertrophic layer increased in each fluorine-exposed group, and the cell column of high fluorine group with low protein and low calcium was disarranged, and a large number of necrotic areas appeared with the prolongation of fluorine exposure time. Nodular proliferation was occasionally seen in proliferation layer cartilage of high fluoride with high aluminum group. Compared with the same experimental group, the number of proliferation layer cell layer of epiphyseal chondrocytes decreased gradually with the prolongation of feeding time (P0.05). The proliferation layer of epiphyseal plate and the number of cell layer in hypertrophic layer of three groups of rats exposed to fluoride in different periods were all more than those in control group, and the number of cell layers increased gradually with the increase of fluoride concentration (P0.05). The proliferation of epiphyseal plate and the number of mast cell layer in high fluorine group with low protein and low calcium were lower than those in high fluoride group. Conclusion the damage of epiphyseal chondrocytes appeared in the rats with coal-burning fluorosis, the number of cell layers in the hypertrophic layer and proliferative layer increased, and a large number of necrotic areas appeared in the epiphyseal plate of the rats with high fluoride and low protein and low calcium. The thickening of epiphyseal plate in high fluoride with high aluminum group was mainly nodular hyperplasia.
【作者单位】： 贵州医科大学;贵州省疾病预防控制中心;福建省疾病预防控制中心;
【基金】：黔科合SY字[2009]3072 黔科合社字[2008]5020号
【分类号】：R599
本文编号：2359255
[Abstract]:Objective to simulate the rat model of coal-burning fluorosis and observe the morphological changes of epiphyseal plate cartilage in different periods. Methods one hundred and fifty SD rats were randomly divided into control group, middle fluoride group, high fluoride with low protein and low calcium group, and high fluoride and high aluminum group with 30 rats in each group (half male and female). The rats were killed on the 60th, 120th and 180th day, respectively. The histopathological changes of the rat epiphyseal plate cartilage were observed by taking the left knee joint. Results compared with the control group, the epiphyseal plate margin was irregular, the calcification of chondrocytes was delayed, the number of cell layers in proliferative layer and hypertrophic layer increased in each fluorine-exposed group, and the cell column of high fluorine group with low protein and low calcium was disarranged, and a large number of necrotic areas appeared with the prolongation of fluorine exposure time. Nodular proliferation was occasionally seen in proliferation layer cartilage of high fluoride with high aluminum group. Compared with the same experimental group, the number of proliferation layer cell layer of epiphyseal chondrocytes decreased gradually with the prolongation of feeding time (P0.05). The proliferation layer of epiphyseal plate and the number of cell layer in hypertrophic layer of three groups of rats exposed to fluoride in different periods were all more than those in control group, and the number of cell layers increased gradually with the increase of fluoride concentration (P0.05). The proliferation of epiphyseal plate and the number of mast cell layer in high fluorine group with low protein and low calcium were lower than those in high fluoride group. Conclusion the damage of epiphyseal chondrocytes appeared in the rats with coal-burning fluorosis, the number of cell layers in the hypertrophic layer and proliferative layer increased, and a large number of necrotic areas appeared in the epiphyseal plate of the rats with high fluoride and low protein and low calcium. The thickening of epiphyseal plate in high fluoride with high aluminum group was mainly nodular hyperplasia.
【作者单位】： 贵州医科大学;贵州省疾病预防控制中心;福建省疾病预防控制中心;
【基金】：黔科合SY字[2009]3072 黔科合社字[2008]5020号
【分类号】：R599
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