类风湿关节炎患者外周血miR-146a、miR-155、Ets-1以及IRAK1的表达及临床意义

发布时间：2018-11-27 07:22

【摘要】：为探讨类风湿关节炎(rheumatoid arthritis,RA)患者PBMC中miR-146a、miR-155、Ets-1和IRAK1的表达水平及与病情活动度的相关性,收集RA高活动期患者23例、低活动期患者21例和健康志愿者22例,采用qRT-PCR检测PBMC中miR-146a、miR-155、Ets-1、IRAK1的相对表达水平,同时分析其与临床及实验室指标的相关性。RA患者PBMC的miR-146a、miR-155相对表达量显著高于健康对照组(P0.05),高活动组高于低活动组(P0.05)、低活动组与健康对照组差异无统计学意义(P0.05);RA患者PBMC的Ets-1表达水平与健康对照组差异无统计学意义,高活动组显著低于低活动组和健康对照组(P0.05)、低活动组与健康对照组差异无统计学意义(P0.05);RA患者PBMC的IRAK1表达水平显著低于健康对照组(P0.05),高活动组显著低于健康对照组(P0.01)、低活动组显著低于健康对照组(P0.05),而高活动组与低活动组间无统计学差异(P0.05)。在RA患者中miR-155表达水平与血沉正相关(r=0.319,P=0.042)而与血红蛋白负相关(r=-0.386,P=0.017);Ets-1与CRP负相关(r=-0.408,P=0.007);IRAK1与RF、DAS 28、总补体负相关(r=-0.513,P=0.001;r=-0.332,P=0.029;r=-0.49,P=0.015)。研究结果显示,RA高活动组患者PBMC中miR-146a、miR-155表达升高,Ets-1、IRAK1表达降低,可能参与了RA发病过程的调控;但本研究样本量有限,miR-146a、miR-155和Ets-1、IRAK1参与RA发病的关系有待后续大样本研究证实,且相关调控机制仍需进一步深入研究。
[Abstract]:To investigate the expression of miR-146a,miR-155,Ets-1 and IRAK1 in PBMC of patients with rheumatoid arthritis (rheumatoid arthritis,RA) and their correlation with disease activity, 23 patients with high activity of RA were collected. The relative expression of miR-146a,miR-155,Ets-1,IRAK1 in PBMC was detected by qRT-PCR in 21 patients with low active stage and 22 healthy volunteers. The correlation between miR-146a,miR-155,Ets-1,IRAK1 expression and clinical and laboratory indexes was also analyzed. MiR-146a, of PBMC in RA patients The relative expression of miR-155 was significantly higher than the healthy control group (P0.05), the high activity group was higher than the low activity group (P0.05), there was no significant difference between the low activity group and the healthy control group (P0.05). There was no significant difference in the expression of PBMC Ets-1 between the RA patients and the healthy control group. The expression of PBMC in the high activity group was significantly lower than that in the low activity group and the healthy control group (P0.05), but there was no significant difference between the low activity group and the healthy control group (P0.05). The level of PBMC IRAK1 expression in RA patients was significantly lower than that in healthy control group (P0.05), high activity group was significantly lower than healthy control group (P0.01), low activity group was significantly lower than healthy control group (P0.05). There was no statistical difference between high activity group and low activity group (P0.05). In RA patients, the expression of miR-155 was positively correlated with erythrocyte sedimentation rate (r = 0.319) and negatively correlated with hemoglobin (r = 0.386, P = 0.017), Ets-1 was negatively correlated with CRP (r = 0.408, P = 0.007). There was a negative correlation between IRAK1 and RF,DAS 28, total complement (r = 0.513, P = 0.001, P = 0.332, P = 0.029, P = 0.49, P = 0.015). The results showed that the expression of miR-146a,miR-155 increased and the expression of Ets-1,IRAK1 decreased in PBMC of patients with high activity of RA, which may be involved in the regulation of the pathogenesis of RA. However, the sample size of this study is limited, the relationship between miR-146a,miR-155 and Ets-1,IRAK1 in the pathogenesis of RA needs to be confirmed in the following large sample studies, and the related regulatory mechanisms need to be further studied.
【作者单位】：南方医科大学输血医学系;南方医科大学南方医院;南方医科大学中医药学院;
【基金】：国家自然科学基金(81603599;31100661) 广东省中医药局科研课题(20151307) 南方医科大学中医药学院2015年度院长基金项目
【分类号】：R593.22

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