糖尿病性耳聋听力变化特点及其发病机制
发布时间：2018-11-29 08:26
【摘要】：第一部分糖尿病性耳聋听力变化特点目的:探讨自发性糖尿病鼠听力变化特点。方法:清洁级雄性,7周龄Zucker Diabetes Fat(ZDF)大鼠根据糖尿病发病与否分为发生糖尿病的ZDF模型鼠(fa/fa)(实验组)10只,不发生糖尿病的ZDF对照鼠(fa/+)(对照组)5只。分别于2、3、4、5、6月大小时测大鼠的体重、空腹血糖、短声诱发听性脑干反应(Click-ABR)、短纯音诱发听性脑干反应(tb-ABR)、畸变产物耳声发射(DPOAEs)。结果:实验鼠在第2月龄内12小时禁食不禁饮测空腹血糖在正常范围,第3月龄时实验鼠的空腹血糖已达糖尿病标准并维持在较高水平;对照组大鼠血糖一直保持在正常范围。根据测得空腹血糖值大小实验组空腹血糖值上升相对较快的鼠(5只)在4月龄时tb-ABR在8k Hz上的阈值较其2月龄时阈值升高(p0.05);Click-ABR 90d BSPLⅠ波潜伏期、Ⅰ-Ⅲ间期较在2月龄时延长(p0.05),4月后Ⅰ波潜伏期、Ⅰ-Ⅲ间期开始缩短。实验组空腹血糖上升相对较慢鼠在5月龄时tb-ABR在8k Hz、16k Hz、32k Hz的阈值较2月龄时阈值升高(p0.05),以32k Hz听力损失最严重,Click-ABR90d BSPLⅠ波及Ⅰ-Ⅲ波潜伏期出现较明显延长(p0.05),5月后Ⅰ波潜伏期、Ⅰ-Ⅲ间期开始缩短。对照组大鼠实验前后听力无明显变化(p0.05)。结论:肥胖ZDF糖尿病大鼠发病前期听力无明显变化,糖尿病鼠发病稳定后1到2个月(即4、5月龄)开始出现较明显的听力损害;糖尿病听力损失的发病进展速度也许与血糖升高的快慢有关;糖尿病鼠高频听力损失较重,提示临床糖尿病人应当监测高频听力变化为主。第二部分糖尿病鼠内耳结构的变化特点目的:分析自发性糖尿病鼠内耳结构变化特点,为糖尿病的发病机制提供基础支持。方法:通过石蜡切片HE染色,耳蜗基底膜铺片的免疫荧光染色及扫描电镜观察糖尿病鼠耳蜗的基本结构、内外毛细胞及其纤毛排列、血管纹的变化情况。结果:石蜡切片HE染色提示ZDF糖尿病鼠血管纹较对照鼠变薄,基底膜铺片及免疫荧光染色观察毛细胞及其纤毛排列无明显变化。扫描电镜观察提示ZDF糖尿病发病鼠与对照鼠顶回均有部分外毛细胞纤毛及其表皮板缺失,缺失部分被上皮样细胞替代,ZDF糖尿病发病鼠与对照鼠比较,其顶回内毛细胞纤毛较对照鼠有明显缺失,表皮板尚完整。ZDF糖尿病发病鼠与对照鼠中回及底回内、外毛细胞纤毛无明显脱落。结论:糖尿病鼠内耳结构免疫组化及电镜上主要以血管纹及内毛细胞损伤为主;内耳大体结构未见明显改变。综合糖尿病鼠听力检测及耳蜗形态结构改变,糖尿病性听力损失特点可能主要表现为隐性听力损失(HHL)。故临床上建议糖尿病病人随访耳纯音测听及言语识别率(特别是噪音下言语识别率)检测,同时注意检测耳蜗电图的变化,以便于更好的观察糖尿病对听力的损伤情况。
[Abstract]:Part I: hearing characteristics of diabetic deafness objective: to investigate the hearing characteristics of spontaneous diabetic rats. Methods: Zucker Diabetes Fat (ZDF) rats of 7 weeks old were divided into 10 ZDF model rats (experimental group) with diabetes mellitus (fa/fa) according to the onset of diabetes. There were 5 ZDF control rats (fa/) without diabetes mellitus. The body weight, fasting blood glucose, auditory brainstem response (Click-ABR), auditory brainstem response (tb-ABR) and distortion product otoacoustic emission (DPOAEs).) were measured at 2: 3 and 4: 5, respectively. Results: the fasting blood glucose was measured in normal range during 12 hours fasting in the second month, and the fasting blood glucose reached the diabetic standard and maintained at a higher level in the third month, while the blood glucose in the control group was maintained in the normal range. The threshold of tb-ABR at 8 k Hz at 4 months was higher in the experimental group than that in the 2 month old group (p0. 05). The latency of BSPL 鈪，
本文编号：2364495
[Abstract]:Part I: hearing characteristics of diabetic deafness objective: to investigate the hearing characteristics of spontaneous diabetic rats. Methods: Zucker Diabetes Fat (ZDF) rats of 7 weeks old were divided into 10 ZDF model rats (experimental group) with diabetes mellitus (fa/fa) according to the onset of diabetes. There were 5 ZDF control rats (fa/) without diabetes mellitus. The body weight, fasting blood glucose, auditory brainstem response (Click-ABR), auditory brainstem response (tb-ABR) and distortion product otoacoustic emission (DPOAEs).) were measured at 2: 3 and 4: 5, respectively. Results: the fasting blood glucose was measured in normal range during 12 hours fasting in the second month, and the fasting blood glucose reached the diabetic standard and maintained at a higher level in the third month, while the blood glucose in the control group was maintained in the normal range. The threshold of tb-ABR at 8 k Hz at 4 months was higher in the experimental group than that in the 2 month old group (p0. 05). The latency of BSPL 鈪，
本文编号：2364495
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