糖尿病足患者外周血T细胞的分化

发布时间：2018-12-06 21:18

【摘要】：目的探讨糖尿病足(DFU)患者外周血中T细胞分化情况及其功能。方法采用流式细胞术分析DFU患者外周血中CD4~+、CD8~+T细胞亚群分布情况,包括初始T细胞、活化T细胞及效应T细胞;分析DFU患者外周血CD4~+、CD8~+T细胞迁移分子CC趋化因子受体(CCR)5、CCR4、CXC趋化因子受体(CXCR)3的表达情况;分析DFU患者外周血CD4~+、CD8~+T细胞分泌炎症因子干扰素(IFN)γ,白细胞介素(IL)2,肿瘤坏死因子(TNF)-α情况。结果与对照组比较,DFU患者外周血初始CD4~+、CD8~+T细胞比例显著降低,活化与效应T细胞比例显著升高(P0.05);迁移分子受体CCR4表达无显著差异(P0.05),CCR5与CXCR3表达在DFU患者中显著降低(P0.05);DFU患者中,炎症因子IFNγ在活化T细胞中高表达,在效应T细胞中几乎不表达,而IFNγ与TNF-α在效应T细胞中高表达,在初始T细胞中几乎不表达。结论DFU患者外周血T细胞亚群发生变化,其分泌的炎症因子影响了T细胞的免疫功能发挥。
[Abstract]:Objective to investigate the differentiation and function of T cells in peripheral blood of diabetic foot patients with (DFU). Methods the distribution of CD4~ and CD8~ T cell subsets in peripheral blood of DFU patients was analyzed by flow cytometry, including initial T cells, activated T cells and effector T cells. The expression of CC chemokine receptor (CCR) 5, CCR4 and CXC chemokine receptor (CXCR) 3 in peripheral blood of CD4~ and CD8~ T cells in patients with DFU was analyzed. The secretion of inflammatory factor interferon (IFN) 纬, interleukin (IL) 2 and tumor necrosis factor (TNF)-伪 by CD4~ and CD8~ T cells in peripheral blood of patients with DFU were analyzed. Results compared with the control group, the proportion of CD4~ and CD8~ T cells in peripheral blood of patients with DFU decreased significantly, and the proportion of activated and effector T cells increased significantly (P0.05). There was no significant difference in the expression of migration molecule receptor CCR4 (P0.05), but the expression of CCR5 and CXCR3 decreased significantly in DFU patients (P0.05). In DFU patients, the inflammatory factor IFN 纬 was highly expressed in activated T cells, but hardly expressed in effector T cells, while IFN 纬 and TNF- 伪 were highly expressed in effector T cells, but hardly in initial T cells. Conclusion the T cell subsets in peripheral blood of DFU patients are changed, and the inflammatory cytokines secreted by them affect the immune function of T cells.
【作者单位】：三峡大学附属仁和医院手足显微外科;
【分类号】：R587.2

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