原发性干燥综合征γδT细胞Vδ2亚群表型及与疾病活动程度的相关性

发布时间：2018-12-11 05:02

【摘要】：目的通过流式细胞技术检测外周血γδT细胞Vδ2亚群的比例和功能表型,研究原发性干燥综合征(primary Sjogren's syndrome,PSS)患者γδT细胞Vδ2细胞亚群数量及功能变化,及其与疾病活动程度的相关性。方法研究对象分为PSS患者27例,疾病对照组原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者10例,健康对照(heal thy control,HC)15例,分别通过流式细胞技术检测其外周血γδT细胞Vδ2亚群占CD3+淋巴细胞和γδT细胞的比例,并检测Vδ2细胞活化、杀伤功能、细胞增殖、细胞凋亡相关功能表型,比较疾病组和对照组之间各指标是否存在显著差异。最后,结合患者临床资料对PSS患者通过疾病活动评估指数(ESSDAI)进行评分,研究V82细胞比例与疾病活动程度的相关性。结果PSS患者组、DC组、HC组Vδ2细胞占CD3+淋巴细胞比例分别为2.152±0.292%,3.790±0.494%,4.900±0.811%,PSS患者组较HC组、DC组均显著降低(P值分别为0.0005,0.0117),而DC组较HC组无明显变化(P值为0.3244)。PSS患者组、DC组、HC组Vδ2细胞占γδT细胞比例分别为60.38±3.930%,75.79±4.717%,78.75±3.719%,PSS患者组较HC组和DC组显著降低(P值为0.0045,0.0199),而DC组较HC组无明显变化(P值为0.6279)。Vδ2细胞表达CD27、CD38、CD69、CD62L、CD45RA、CD45RO、HLA-DR、肿瘤坏死因子-α(TNF-α)、干扰素-r(IFN-r)、颗粒酶(Granzyme A/B)和穿孔素(perforin)、Ki-67、7-AAD等细胞活化、功能、增殖相关分子,且与健康对照组无明显差异。而PSS患者组凋亡相关分子Annexin表达显著增加。PSS患者外周血淋巴细胞中Vδ2细胞所占比例与基于ESSDAI评分的疾病活动程度呈线性负相关。结论与HC和DC组相比,PSS患者外周血Vδ2细胞占CD3+淋巴细胞和γδT细胞的比例明显下降。Vδ2细胞占CD3+淋巴细胞比例与基于ESSDAI评分的疾病活动程度呈线性负相关。HC组相比,PSS患者Vδ2细胞除凋亡相关分子Annexin Ⅴ表达显著增加外,其他细胞功能表型表达无明显变化。
[Abstract]:Objective to study the number and function of V 未 2 subsets of 纬 未 T cells in patients with primary Sjogren's syndrome (primary Sjogren's syndrome,PSS) by detecting the proportion and function of V 未 2 subsets of 纬 未 T cells by flow cytometry. And its correlation with the degree of disease activity. Methods the subjects were divided into three groups: 27 patients with PSS, 10 patients with primary biliary cirrhosis (primary biliary cirrhosis,PBC) and 15 healthy controls (heal thy control,HC). The ratio of V 未 2 subsets of 纬 未 T cells to CD3 lymphocytes and 纬 未 T cells was detected by flow cytometry, and the activation, killing function, cell proliferation and apoptosis related functional epitopes of V 未 2 cells were detected. To compare whether there were significant differences between the disease group and the control group. Finally, the relationship between the proportion of V82 cells and the degree of disease activity was studied by scoring the disease activity assessment index (ESSDAI) in patients with PSS. Results the ratio of V 未 2 cells to CD3 lymphocytes in PSS group, DC group and HC group were 2.152 卤0.292and 3.790 卤0.4944.900 卤0.8114g, respectively. The percentage of V 未 2 cells in DC group was significantly lower than that in HC group (P = 0.0005, 0.0117, respectively). The ratio of V 未 2 cells to 纬 未 T cells in DC group, DC group and DC group was 60.38 卤3.930 and 75.79 卤4.717 78.75 卤3.719, respectively. The expression of CD27,CD38,CD69,CD62L,CD45RA,CD45RO,HLA-DR, in PSS group was significantly lower than that in HC group and DC group (P = 0.0045,0.0199), but there was no significant change in DC group compared with HC group (P = 0.6279). V 未 2 cell expression). Tumor necrosis factor- 伪 (TNF- 伪), interferon- r (IFN-r), granzyme (Granzyme A / B) and perforin (perforin), Ki-67,7-AAD were activated, functionally and proliferation-related molecules were not significantly different from those in healthy control group. The proportion of V 未 2 cells in peripheral blood lymphocytes of PSS patients was negatively correlated with the degree of disease activity based on ESSDAI score. Conclusion compared with HC and DC groups, the percentage of V 未 2 cells in CD3 lymphocytes and 纬 未 T cells in peripheral blood of PSS patients was significantly decreased, and the proportion of V 未 2 cells to CD3 lymphocytes was linearly negatively correlated with the disease activity based on ESSDAI score. Compared with HC group, the percentage of V 未 2 cells in CD3 lymphocytes and 纬 未 T cells in PSS patients was significantly decreased. In patients with PSS, the expression of Annexin 鈪，

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