吡格列酮对PCOS卵巢颗粒细胞分泌雌激素的影响

发布时间：2018-12-11 19:19

【摘要】：目的观察吡格列酮对多囊卵巢综合征(PCOS)患者卵巢颗粒细胞雌激素分泌功能和其上P450芳香化酶(P450arom)、胰岛素受体底物-1(IRS-1)和胰岛素受体底物-2(IRS-2)表达的影响。方法对27例行体外受精-胚胎移植PCOS患者的颗粒细胞进行体外原代培养并分组,A组分别加入不同浓度(0、10、102、103、104nmol/L)吡格列酮、B组为不同浓度吡格列酮与卵泡刺激素(FSH,50ng/L)及C组为不同浓度吡格列酮与胰岛素样生长因子-Ⅰ(IGF-Ⅰ,50ng/L)。用放射免疫法检测培养上清液中雌二醇(E2)浓度,Real-time PCR法检测颗粒细胞上P450arom、IRS-1、IRS-2mRNA的表达变化。结果在A、B、C组中,人PCOS卵巢颗粒细胞培养48h的E2产生量差异有统计学意义(P0.05),吡格列酮各浓度组间P450arom mRNA表达量差异有统计学意义(P0.05),随着吡格列酮浓度的增加,E2水平和P450arom mRNA表达也随之下降;人PCOS卵巢颗粒细胞在相同浓度吡格列酮时,C组E2水平和P450arom mRNA表达明显高于B组(P0.01)及A组(P0.01),B组E2产生量和P450arom mRNA表达高于A组,差异有统计学意义(P0.05);人PCOS卵巢颗粒细胞经不同浓度吡格列酮刺激48h,各浓度组间IRS-1、IRS-2mRNA表达差异有统计学意义(P0.05),随着吡格列酮浓度的增加,IRS-1mRNA表达随之下降,IRS-2mRNA表达随之上升。结论吡格列酮可能直接或间接通过对P450arom的抑制,减少E2的生成,改善颗粒细胞上IRS-1和IRS-2表达的失衡,抑制PCOS卵巢的局部胰岛素抵抗状态;吡格列酮可抑制FSH和IGF-Ⅰ刺激卵巢颗粒细胞分泌雌激素的功能。
[Abstract]:Objective to observe the effects of pioglitazone on estrogen secretion and P450 aromatase (P450arom) in granulosa cells of patients with polycystic ovary syndrome (PCOS). Effects of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2) expression. Methods the granulosa cells of 27 PCOS patients undergoing in vitro fertilization and embryo transfer were cultured in vitro and divided into two groups. Group A was treated with different concentrations of pioglitazone (0102103104nmol / L), group B was treated with different concentrations of pioglitazone and follicle stimulating hormone (FSH,). 50ng/L and group C were treated with different concentrations of pioglitazone and IGF- 鈪，

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