贝那普利对早期糖尿病肾脏疾病足细胞损伤保护作用的实验研究
发布时间：2018-12-12 13:51
【摘要】：目的探讨贝那普利对早期糖尿病肾脏疾病(DKD)足细胞损伤保护作用。方法选取SPF级雄性Wistar大鼠40只,随机分为正常对照(NC)组、单肾切除(SNE)组、DKD组和贝那普利(Ben)组,每组10只,其中DKD组和Ben组采用60mg/kg STZ腹腔注射建立DKD模型,成模4周后,Ben组予10mg/(kg·d)贝那普利灌胃,DKD组、SNE组和NC组4周后予等量蒸馏水灌胃。检测各组生化指标,观察肾脏组织病理改变及足细胞超微结构,免疫荧光检测肾皮质Nephrin和Podocin蛋白表达。结果Ben组多饮多尿症状减轻,第4、8、12周体质量均高于DKD组(P0.05);DKD组和Ben组血糖高于NC组、SNE组(P0.05);DKD组Scr和24h尿蛋白定量高于NC组、SNE组,血清白蛋白低于NC组、单肾切除组(P0.05);Ben组Scr和24h尿蛋白定量(81.30±20.46)μmol/L、(190.44±5.10)mg/24h低于DKD组,血清白蛋白(36.47±1.32)g/L高于DKD组(P0.05);DKD组肾组织AT Ⅱ高于NC组(P0.05);Ben组AT Ⅱ(14.60±0.82)pg/ml低于DKD组(P0.05);DKD模型组足突宽度、足突融合率和基底膜厚度高于NC组、SNE组(P0.05);Ben组足突宽度、足突融合率和基底膜厚度分别为(0.41±0.01)μm、(34.20%±6.81%)和(0.40±0.03)μm,低于DKD组(P0.05);Ben组Nephrin和Podocin蛋白表达较DKD组升高。结论贝那普利对早期DKD足细胞有保护作用,其机制可能与降低肾组织ATⅡ,上调Nephrin和Podocin蛋白表达有关。
[Abstract]:Objective to investigate the protective effect of benazepril on (DKD) podocyte injury in early diabetic nephropathy. Methods Forty SPF grade male Wistar rats were randomly divided into normal control (NC) group, single nephrectomy (SNE) group, DKD group and benazepril (Ben) group with 10 rats in each group. DKD model was established by 60mg/kg STZ intraperitoneal injection in DKD and Ben groups. After 4 weeks, Ben group was given 10mg/ (kg d) benazepril, DKD group, SNE group and NC group were given the same amount of distilled water after 4 weeks. The pathological changes of renal tissue and the ultrastructure of podocytes were observed. The expressions of Nephrin and Podocin protein in renal cortex were detected by immunofluorescence. Results the symptoms of polydipsia and polyuria were alleviated in Ben group, and the body mass of DKD group was higher than that of DKD group at the 12th week (P0.05). The blood glucose in); DKD group and Ben group was higher than that in NC group and SNE group (P0.05). The levels of Scr and 24 hours urinary protein in DKD group were higher than those in NC group, while in SNE group, serum albumin levels were lower than those in NC group and single nephrectomy group (P0.05). The levels of Scr and 24-hour urinary protein in Ben group (81.30 卤20.46) 渭 mol/L, (190.44 卤5.10) mg/24h were lower than those in DKD group, and serum albumin (36.47 卤1.32) g / L was higher than DKD group (P0.05). The renal tissue AT 鈪，
本文编号：2374691
[Abstract]:Objective to investigate the protective effect of benazepril on (DKD) podocyte injury in early diabetic nephropathy. Methods Forty SPF grade male Wistar rats were randomly divided into normal control (NC) group, single nephrectomy (SNE) group, DKD group and benazepril (Ben) group with 10 rats in each group. DKD model was established by 60mg/kg STZ intraperitoneal injection in DKD and Ben groups. After 4 weeks, Ben group was given 10mg/ (kg d) benazepril, DKD group, SNE group and NC group were given the same amount of distilled water after 4 weeks. The pathological changes of renal tissue and the ultrastructure of podocytes were observed. The expressions of Nephrin and Podocin protein in renal cortex were detected by immunofluorescence. Results the symptoms of polydipsia and polyuria were alleviated in Ben group, and the body mass of DKD group was higher than that of DKD group at the 12th week (P0.05). The blood glucose in); DKD group and Ben group was higher than that in NC group and SNE group (P0.05). The levels of Scr and 24 hours urinary protein in DKD group were higher than those in NC group, while in SNE group, serum albumin levels were lower than those in NC group and single nephrectomy group (P0.05). The levels of Scr and 24-hour urinary protein in Ben group (81.30 卤20.46) 渭 mol/L, (190.44 卤5.10) mg/24h were lower than those in DKD group, and serum albumin (36.47 卤1.32) g / L was higher than DKD group (P0.05). The renal tissue AT 鈪，
本文编号：2374691
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