葡萄糖转运蛋白2在人脐带间充质干细胞向胰岛前体细胞分化过程中表达的变化

发布时间：2018-12-14 19:24

【摘要】：目的探讨葡萄糖转运蛋白2(Glut2)在人脐带间充质干细胞(h UMSCs)向胰岛前体细胞分化过程中的表达变化。方法分离、培养、鉴定及诱导h UMSCs,分别收集诱导过程中第7天、14天和21天细胞和细胞上清液,采用免疫细胞化学、ELISA、免疫荧光、Western blotting及Real-time PCR检测诱导后细胞相关蛋白和基因的表达。结果免疫组织化学检测诱导后细胞胰腺十二指肠同源盒基因-1(PDX-1呈阳性表达;免疫荧光检测诱导后细胞Ngn3和胰岛素呈阳性表达;Western blotting检测Glut2在诱导过程中逐渐升高,诱导14 d达到峰值,与正常组比较P0.01;Real-time PCR显示,Glut2基因自第7天即增高(P0.05)。结论 h UMSCs经诱导后分化为胰岛前体细胞,表达Glut2后能引起胰岛素分泌,已初步具有胰岛B细胞功能。
[Abstract]:Objective to investigate the expression of glucose transporter 2 (Glut2) in the differentiation of human umbilical cord mesenchymal stem cells (h UMSCs) into islet precursor cells. Methods the supernatants of cells and cells were collected on the 7th, 14th and 21st day during the induction by UMSCs,. The supernatants were collected by immunocytochemistry and ELISA, immunofluorescence. Western blotting and Real-time PCR were used to detect the expression of cell related proteins and genes. Results the positive expression of PDX-1 was detected by immunohistochemistry, and the expression of Ngn3 and insulin was detected by immunofluorescence. Western blotting detection of Glut2 increased gradually during induction and reached its peak at 14 days after induction. Compared with the normal group, P0.01Real-time PCR showed that the Glut2 gene increased from the 7th day (P0.05). Conclusion h UMSCs can differentiate into islet precursor cells after induction and expression of Glut2 can induce insulin secretion, which has the function of islet B cells.
【作者单位】：贵州医科大学组织学胚胎学教研室;武汉大学医学研究院;
【基金】：国家自然科学基金(81160099)
【分类号】：R587.1

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