氯化镉短期重复染毒致雄性大鼠早期毒性损伤敏感指标

发布时间：2018-12-16 22:30

【摘要】：目的观察氯化镉对雄性大鼠经口灌胃短期重复染毒的早期毒性损伤效应指标。方法无特定病原体级健康雄性SD大鼠40只,随机分成对照组和低、中、高剂量组。采用经口灌胃染毒方法,低、中、高剂量组大鼠分别予剂量为1.11、3.51和11.06 mg/kg体质量的氯化镉染毒,1次/d,连续4周;对照组予等体积超纯水处理。染毒期间称量大鼠体质量,观察其活动状况。实验结束后处死大鼠,检测其主要脏器系数、血常规、血清生化指标、尿中相关效应指标和骨密度指标。结果染毒期间,高剂量组大鼠出现活动减少、静卧多、行动迟缓和皮毛失去光泽等表现,第1~4周体质量均低于同时间点对照组(P0.05)。染毒结束后,与对照组比较,高剂量组大鼠肾脏和脾脏的质量均下降(P0.05),肝脏脏器系数升高(P0.05);中和高剂量组大鼠血液、尿液、肝脏、肾脏和股骨中的镉水平均升高(P0.05);低和中剂量组大鼠红细胞计数均升高(P0.05);中和高剂量组大鼠血红蛋白水平均下降(P0.05),高剂量组大鼠血清丙氨酸氨基转移酶活力升高(P0.05)。与对照组比较,中剂量组大鼠尿中α_1-微球蛋白和β_2-微球蛋白水平均下降(P0.05),尿素氮水平升高(P0.05),但高剂量组大鼠上述指标均无出现有统计学意义的改变(P0.05),且3个剂量组大鼠尿中N-乙酰-β-D氨基葡萄糖苷酶水平均无出现有统计学意义的改变(P0.05)。高剂量组大鼠股骨质量、骨钙水平、中点骨密度和远端骨密度均低于对照组(P0.05)。结论骨骼效应可作为氯化镉短期重复实验的早期毒性效应指标。
[Abstract]:Objective to observe the early toxicity damage index of cadmium chloride on male rats. Methods Forty healthy male SD rats without specific pathogens were randomly divided into control group, low dose group, middle dose group and high dose group. The rats in the low, middle and high dose groups were treated with cadmium chloride with the body mass of 1.113.51 and 11.06 mg/kg, once a day for 4 weeks, and the control group was treated with iso-volume ultrapure water. The body mass of rats was measured during exposure and their activities were observed. At the end of the experiment, the rats were killed and the main organ coefficients, blood routine, serum biochemical indexes, urine related effect indexes and bone mineral density indexes were detected. Results during the period of exposure, the rats in the high dose group showed decreased activity, more resting, retarded movement and lost the luster of the fur, and the body mass at the 1st week was lower than that in the control group at the same time point (P0.05). After exposure, compared with the control group, the weight of kidney and spleen decreased (P0.05), liver organ coefficient increased (P0.05) in high dose group. The levels of cadmium in blood, urine, liver, kidney and femur of rats in medium and high dose groups were all increased (P0.05), red blood cell count of rats in low and medium dose groups were all increased (P0.05). The level of hemoglobin in middle and high dose groups decreased (P0.05), and the activity of serum alanine aminotransferase increased in high dose group (P0.05). Compared with the control group, the levels of 伪 _ 1-microglobulin and 尾 _ 2-microglobulin in urine of middle dose group decreased (P0.05), urea nitrogen level increased (P0.05). However, there was no significant change in the above indexes in the high dose group (P0.05), and the levels of N- acetyl-尾-D glucosaminidase in urine of the three dose groups were not significantly changed (P0.05). The femur mass, bone calcium level, midpoint bone mineral density and distal bone mineral density in the high dose group were lower than those in the control group (P0.05). Conclusion skeletal effect can be used as an early toxicity index of cadmium chloride short-term repeated test.
【作者单位】：广东省疾病预防控制中心;
【基金】：广东省科技计划项目(2013B021500014)
【分类号】：R135.1

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