高浓度葡萄糖对C57小鼠视网膜神经节细胞的视觉反应特性的影响——一项离体生理研究
发布时间：2018-12-17 04:01
【摘要】：本研究旨在观察视网膜神经节细胞在高浓度葡萄糖下的视觉反应特性.实验中,视杯平铺于记录腔,Ames缓冲液灌流,单细胞外记录小鼠(Mus musculus)视网膜神经节细胞.实验结果表明,高糖条件下,ON神经节细胞的平均感受野大小(34.1±2.9,n=14)明显小于OFF神经节细胞的(49.3±0.3,n=12)(P0.0001).高渗条件下,可以观察到类似的模式,即ON神经节细胞的平均感受野大小小于OFF的(P0.0001).ON神经节细胞的平均亮度阈值在高糖(P0.0001)或者高渗(P0.0002)条件下明显升高.OFF神经节细胞的平均亮度阈值在相同条件下(高糖:P0.01;高渗:P0.0002)也有升高.在高渗条件下,ON神经节细胞的平均对比增益明显低于OFF神经节细胞的(P0.015).而在高糖条件下,ON神经节细胞的平均增益明显高于OFF神经节细胞的(P0.0001).这些结果表明,高糖可缩小神经节细胞的感受野,降低亮度敏感度,减弱对比增益.高糖对ON和OFF神经节细胞的影响可能是通过不同的机制进行的.
[Abstract]:The aim of this study was to observe the visual response of retinal ganglion cells at high glucose concentration. In the experiment, the (Mus musculus) retinal ganglion cells of mice were recorded in the visual cup, the Ames buffer was perfused with the recording chamber, and the (Mus musculus) retinal ganglion cells were recorded outside the single cell. The results showed that the average receptive field size of ON ganglion cells (34.1 卤2.9) was significantly lower than that of OFF ganglion cells (49.3 卤0.3) (P0.0001). Similar patterns can be observed under hypertonic conditions. That is, the average receptive field size of ON ganglion cells was smaller than that of OFF (P0.0001). ON ganglion cells), the mean luminance threshold of). ON ganglion cells increased significantly under high glucose (P0.0001) or hyperosmotic (P0.0002) conditions. The mean luminance threshold was in the same condition (high sugar: P0.01; Hyperosmotic: P0.0002) also increased. Under hypertonic condition, the average contrast gain of ON ganglion cells was significantly lower than that of OFF ganglion cells (P0.015). At high glucose, the average gain of ON ganglion cells was significantly higher than that of OFF ganglion cells (P0.0001). These results suggest that high glucose can reduce the receptive field, brightness sensitivity and contrast gain of ganglion cells. The effects of high glucose on ON and OFF ganglion cells may be mediated by different mechanisms.
【作者单位】： 北京大学基础医学院人体解剖与组织胚胎学系;湖南师范大学第一附属医院湖南省人民医院马王堆院区;
【分类号】：R587.2
[Abstract]:The aim of this study was to observe the visual response of retinal ganglion cells at high glucose concentration. In the experiment, the (Mus musculus) retinal ganglion cells of mice were recorded in the visual cup, the Ames buffer was perfused with the recording chamber, and the (Mus musculus) retinal ganglion cells were recorded outside the single cell. The results showed that the average receptive field size of ON ganglion cells (34.1 卤2.9) was significantly lower than that of OFF ganglion cells (49.3 卤0.3) (P0.0001). Similar patterns can be observed under hypertonic conditions. That is, the average receptive field size of ON ganglion cells was smaller than that of OFF (P0.0001). ON ganglion cells), the mean luminance threshold of). ON ganglion cells increased significantly under high glucose (P0.0001) or hyperosmotic (P0.0002) conditions. The mean luminance threshold was in the same condition (high sugar: P0.01; Hyperosmotic: P0.0002) also increased. Under hypertonic condition, the average contrast gain of ON ganglion cells was significantly lower than that of OFF ganglion cells (P0.015). At high glucose, the average gain of ON ganglion cells was significantly higher than that of OFF ganglion cells (P0.0001). These results suggest that high glucose can reduce the receptive field, brightness sensitivity and contrast gain of ganglion cells. The effects of high glucose on ON and OFF ganglion cells may be mediated by different mechanisms.
【作者单位】： 北京大学基础医学院人体解剖与组织胚胎学系;湖南师范大学第一附属医院湖南省人民医院马王堆院区;
【分类号】：R587.2
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