母代营养过剩对雄性子代能量代谢的影响

发布时间：2018-12-21 12:04

【摘要】：目的探究母代营养过剩对雄性子代能量代谢的影响。方法 C57BL/6J雌鼠随机分为高脂饮食组和正常饮食组,分别饲喂高脂或正常饲料2个月,然后与正常饮食C57BL/6J雄鼠交配,孕鼠在孕期和哺乳期继续摄取高脂或正常饲料。子一代雄鼠性成熟后与正常饮食C57BL/6J雌鼠交配得到子二代,子二代雄鼠性成熟后再与正常饮食C57BL/6J雌鼠交配得到子三代。测定子一代、子二代、子三代雄性小鼠体重、摄食量及随机血糖值。对子一代和子二代成年雄性小鼠进行腹腔糖耐量实验并记录肝脏、附睾周脂肪、皮下脂肪湿重值。检测子二代成年雄性小鼠血清胰岛素及下丘脑能量代谢相关基因表达情况。结果营养过剩母鼠子一代和子二代雄性小鼠的体重、摄食量、能量摄入量及随机血糖值均高于对照组(均P0.05),子三代之间的差异则无统计学意义(P0.05)。营养过剩母鼠子一代和子二代的成年雄性小鼠的附睾周脂肪脂体比、皮下脂肪脂体比、肝体比及糖耐量曲线下面积均高于对照组(均P0.05)。营养过剩母鼠子二代成年雄性小鼠的血清胰岛素浓度及下丘脑神经肽Y(NPY)、刺鼠相关蛋白(AGRP)、甘丙肽(GAL)、可卡因-安他非明转录调节肽(CART)、SIM1基因的表达量均高于对照组(均P0.05)。结论高脂饮食诱导的营养过剩母代对雄性子代能量代谢的影响可持续至子二代,引起子二代下丘脑能量代谢相关基因表达异常。
[Abstract]:Objective to investigate the effect of maternal excess nutrition on energy metabolism of male offspring. Methods C57BL/6J female rats were randomly divided into high fat diet group and normal diet group, fed with high fat diet or normal diet for 2 months, then mating with normal diet C57BL/6J male rats. Pregnant rats continued to take high fat or normal diet during pregnancy and lactation. After sexual maturation, the male mice of the first generation mate with the normal diet C57BL/6J female mice to obtain the second generation, and the second generation of the male mice after the sexual maturation of the second generation then mate with the normal diet C57BL/6J female mice to get the third generation. The body weight, food intake and random blood sugar of the male mice of the second generation, the second generation and the third generation were measured. The intraperitoneal glucose tolerance test was carried out in the second generation and the second generation of adult male mice, and the wet weight of liver, periepididymal fat and subcutaneous fat were recorded. Serum insulin and hypothalamic energy metabolism related genes were detected in the second generation adult male mice. Results the body weight, food intake, energy intake and random blood glucose of the male mice of the second generation and the second generation were higher than those of the control group (P0.05), but there was no significant difference between the three generations (P0.05). The ratio of periepididymal lipids to lipids, subcutaneous lipids to lipids, livers to lipids and the area under the glucose tolerance curve of adult male mice with over nutrition were higher than those of the control group (P0.05). Serum insulin concentration and hypothalamic neuropeptide Y (NPY), associated protein (AGRP), glycine (GAL), (GAL), cocaine-(CART), in adult male mice of second generation over-fed female mice The expression of SIM1 gene was higher than that of control group (P0.05). Conclusion the effect of high fat diet on the energy metabolism of male offspring can be sustained up to the second generation, resulting in abnormal expression of hypothalamic energy metabolism related genes in the second generation of hypothalamus.
【作者单位】：华中科技大学同济医学院公共卫生学院毒理学系;
【基金】：华中科技大学青年教师基金资助项目(No.2015QN110)
【分类号】：R589

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