西藏移居内地的藏族和汉族不适症状大学生血清蛋白表达分析

发布时间：2018-12-31 15:59

【摘要】：背景随着世居高原人群往返于高原与平原之间愈发频繁,高原低氧人群对平原富氧环境的适应逐渐引起人们的重视。目的基于血清蛋白组学对移居内地的藏、汉族不适症状大学生的血清蛋白表达进行分析,以期为西藏移居内地的藏、汉族大学生因适应不良而引起的疾病提供简单易行的、精确的早期诊断指标,为其临床治疗提供分子靶标。方法分别选取2013年10—11月、2014年10—11月、2015年10—11月西藏民族大学符合纳入标准的藏、汉族不适症状大学生32例为研究对象。按照研究对象民族,将其分为藏族组和汉族组,各16例。收集研究对象血清样本,采用弱阳离子磁珠(MB-WCX)捕获血清蛋白多肽,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术进行质谱分析并比较两组血清蛋白表达水平。结果单样本质谱分析结果显示,3次重复取样,藏族组同一个体分子量为1 000~10 000 m/z的血清蛋白多肽曲线基本趋于一致,汉族组得出相似结果;藏族组与汉族组血清蛋白表达水平存在显著差异。整体样本质谱分析结果显示,藏族组与汉族组分子量为1 000~10 000 m/z的血清蛋白表达水平存在显著差异;对其蛋白多肽成分分析,则呈现出相互聚集、较少重合的现象;藏族组与汉族组分子量为905.65、1 062.10、1 078.11、2 138.79、2 313.40、4 094.67、4 172.89、4 213.96、5 910.71 m/z的血清蛋白表达峰值间存在显著差异(P0.05)。结论西藏移居内地的藏、汉族不适症状大学生血清蛋白表达存在一定差异,其中分子量为4 094.67、2 138.79、905.65 m/z的血清蛋白表达差异较大,为进一步鉴定和定位差异性蛋白的序列和基因奠定了基础。
[Abstract]:Background as the commute between the plateau and the plain becomes more and more frequent, the adaptation of the people in the plateau to the oxygen-enriched environment in the plain gradually attracts people's attention. Objective to analyze the expression of serum protein in Tibetan and Han unwell symptom college students based on seroproteomics, so as to provide a simple and easy way for Tibetan students to emigrate to Tibet and Han nationality college students for diseases caused by maladjustment. The accurate early diagnosis index provides the molecular target for the clinical treatment. Methods from October to November 2013, October to November 2014 and October to November 2015, 32 college students with unwell symptoms of Han nationality were selected as subjects. According to the nationalities of the subjects, they were divided into Tibetan group and Han group, 16 cases each. Serum samples were collected and serum protein peptides were captured by weak cationic magnetic beads (MB-WCX). Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) technique was used to analyze the expression of serum proteins in two groups. Results the results of single sample mass spectrometry showed that the curve of serum protein polypeptide with a molecular weight of 1 000 ~ 10 000 m / z in the Tibetan group was almost the same as that in the Han group, and the same result was obtained in the Han nationality group. There was significant difference in the expression of serum protein between Tibetan group and Han nationality group. The results of mass spectrometry showed that there was a significant difference between Tibetan group and Han group in the expression of serum protein with a molecular weight of 10 000 m / z, and the analysis of protein polypeptide showed a phenomenon of mutual aggregation and less overlap. There was significant difference in the peak expression of serum protein between the Tibetan group and the Han nationality group when the molecular weight was 905.65 (106.102.102.108.1110) 2138.79 (2313.404094.67) and 4172.89 (4213.966) 5 910.71 m / z (P0.05). Conclusion there are some differences in the expression of serum protein between Han Chinese and Tibetan who emigrated to the mainland of Tibet, and there is a significant difference in the expression of serum protein with molecular weight of 4.094.672138.79905.65mz. It provides a basis for further identification and localization of the sequences and genes of differential proteins.
【作者单位】：西藏民族大学医学部;
【基金】：国家自然科学基金资助项目(81360299) 西藏自治区自然科学基金资助项目(2016ZR15-17)
【分类号】：R594.3

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