外周血Th17、Treg、Tfh的变化在桥本甲状腺炎中的意义
发布时间：2019-01-02 13:15
【摘要】：目的:观察桥本甲状腺炎(HT)、Graves病(GD)患者外周血中滤泡辅助性T细胞(Tfh)、辅助性T细胞(Th)17、CD4+CD25+调节性T细胞(Treg),探讨HT的免疫学机制。方法:2013年3月-2014年12月我院门诊及住院收治HT患者50例,GD患者36例;该段时期于我院进行健康体检的对照组30例。通过流式细胞术检测三组人群外周血中Tfh、Th17、Treg的数目及Tfh/CD4+T、Th17/CD4+T、Treg/CD4+T细胞百分率。结果:(1)Tfh/CD4+T细胞百分率:HT组(14.31±2.19)%、GD组(12.98±3.61)%;两组明显高于健康对照组(6.35±1.11)%,差异有统计学意义(P0.05),HT患者组与GD患者组差异无统计学意义(P0.05)。(2)Th17/CD4+T细胞百分率:HT组(2.01±0.48)%、GD组(1.90±1.00)%;两组均高于健康对照组(0.57±0.23)%,差异有统计学意义(P0.05),HT患者组与GD患者组差异无统计学意义(P0.05)。(3)Treg/CD4+T细胞百分率:HT组(2.90±1.04)%;GD组(2.58±1.51)%;两组均低于健康对照组(4.43±0.57)%,差异有统计学意义(P0.05),HT患者组与GD患者组差异无统计学意义(P0.05)。(4)HT组Tfh/CD4+T细胞百分率与TPOAb、TGAb水平呈正相关(r=0.31,p0.05;r=0.33,p0.05),Th17/CD4+T细胞百分率与TPOAb水平呈正相关(r=0.43,p0.05),与TGAb水平无明显相关性(r=0.19,p0.05),Treg/CD4+T细胞百分率与TPOAb水平有负相关趋势,无统计学意义(r=0.3,p0.05),与TGAb水平无明显相关性(r=0.14,p0.05)。结论:HT患者组、GD患者组外周血中Tfh细胞、Th17/CD4+T细胞百分率明显高于健康对照组,且在HT患者组中Tfh/CD4+T细胞百分率与TPOAb、TGAb水平呈正相关,Th17/CD4+T细胞百分率与TPOAb水平呈正相关;而HT患者组、GD患者组外周血中Treg/CD4+T细胞百分率明显低于健康对照组,且Treg/CD4+T细胞百分率与TPOAb有负相关趋势,无统计学意义,Tfh细胞、Th17细胞、Treg细胞三者与对临床上诊断HT最为重要的相关抗体TPOAb、TGAb相关,这也说明通过检测Tfh细胞、Th17细胞、Treg细胞可能对更好的了解HT的发病机制有一定意义。
[Abstract]:Objective: to investigate the immunological mechanism of follicular helper T cells (Tfh), (Tfh), helper T cells (Th) 17), CD4 CD25 regulatory T cells (Treg),) in peripheral blood of patients with Hashimoto's thyroiditis (HT), Graves disease (GD). Methods: from March 2013 to December 2014, 50 patients with HT and 36 patients with GD were treated in our hospital. The number of Tfh,Th17,Treg in peripheral blood of the three groups and the percentage of Th17 / Treg-CD4 / Treg-T T lymphocytes in Tfh/CD4 Th _ 4 / T ~ + T _ 4 were measured by flow cytometry. Results: (1) the percentage of Tfh/CD4 T cells was (14.31 卤2.19)% in HT group and (12.98 卤3.61)% in GD group. The difference between the two groups was statistically significant (P0.05), which was significantly higher than that in the healthy control group (6.35 卤1.11)%. There was no significant difference between HT group and GD group (P0.05). (2) percentage of Th17/CD4 T cells: HT group (2.01 卤0.48)%, GD group (1.90 卤1.00)%; Both groups were significantly higher than the healthy control group (0.57 卤0.23)%, the difference was statistically significant (P0.05). There was no significant difference between HT group and GD group (P0.05). (3) percentage of Treg/CD4 T cells: HT group (2.90 卤1.04)%; In GD group (2.58 卤1.51)%; Both groups were lower than the healthy control group (4.43 卤0.57)%, the difference was statistically significant (P0.05 between), HT group and GD group). There was no significant difference between the two groups (P0.05). (4) the percentage of Tfh/CD4 T cells and TPOAb, in HT group. The level of TGAb was positively correlated (r = 0.31, p 0.05); The percentage of Th17/CD4 T cells was positively correlated with the level of TPOAb (r = 0.43, p0.05), but not with the level of TGAb (r = 0.19, p 0.05). The percentage of Treg/CD4 T cells was negatively correlated with the level of TPOAb. There was no statistical significance (r = 0.3p 0.05), but no significant correlation with the level of TGAb (r = 0.14p 0.05). Conclusion: the percentage of Tfh cells and Th17/CD4 T cells in peripheral blood of HT patients and GD patients is significantly higher than that of healthy controls, and the percentage of Tfh/CD4 T cells in HT patients is positively correlated with TPOAb,TGAb level. The percentage of Th17/CD4 T cells was positively correlated with the level of TPOAb. However, the percentage of Treg/CD4 T cells in peripheral blood of HT patients and GD patients was significantly lower than that of healthy controls, and the percentage of Treg/CD4 T cells was negatively correlated with TPOAb. There was no statistical significance between the percentage of Treg/CD4 T cells and TPOAb. The relationship between Treg cells and TPOAb,TGAb, which is the most important antibody for the diagnosis of HT, also indicates that the detection of Tfh cells, Th17 cells and Treg cells may be helpful to better understand the pathogenesis of HT.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R581.4
本文编号：2398536
[Abstract]:Objective: to investigate the immunological mechanism of follicular helper T cells (Tfh), (Tfh), helper T cells (Th) 17), CD4 CD25 regulatory T cells (Treg),) in peripheral blood of patients with Hashimoto's thyroiditis (HT), Graves disease (GD). Methods: from March 2013 to December 2014, 50 patients with HT and 36 patients with GD were treated in our hospital. The number of Tfh,Th17,Treg in peripheral blood of the three groups and the percentage of Th17 / Treg-CD4 / Treg-T T lymphocytes in Tfh/CD4 Th _ 4 / T ~ + T _ 4 were measured by flow cytometry. Results: (1) the percentage of Tfh/CD4 T cells was (14.31 卤2.19)% in HT group and (12.98 卤3.61)% in GD group. The difference between the two groups was statistically significant (P0.05), which was significantly higher than that in the healthy control group (6.35 卤1.11)%. There was no significant difference between HT group and GD group (P0.05). (2) percentage of Th17/CD4 T cells: HT group (2.01 卤0.48)%, GD group (1.90 卤1.00)%; Both groups were significantly higher than the healthy control group (0.57 卤0.23)%, the difference was statistically significant (P0.05). There was no significant difference between HT group and GD group (P0.05). (3) percentage of Treg/CD4 T cells: HT group (2.90 卤1.04)%; In GD group (2.58 卤1.51)%; Both groups were lower than the healthy control group (4.43 卤0.57)%, the difference was statistically significant (P0.05 between), HT group and GD group). There was no significant difference between the two groups (P0.05). (4) the percentage of Tfh/CD4 T cells and TPOAb, in HT group. The level of TGAb was positively correlated (r = 0.31, p 0.05); The percentage of Th17/CD4 T cells was positively correlated with the level of TPOAb (r = 0.43, p0.05), but not with the level of TGAb (r = 0.19, p 0.05). The percentage of Treg/CD4 T cells was negatively correlated with the level of TPOAb. There was no statistical significance (r = 0.3p 0.05), but no significant correlation with the level of TGAb (r = 0.14p 0.05). Conclusion: the percentage of Tfh cells and Th17/CD4 T cells in peripheral blood of HT patients and GD patients is significantly higher than that of healthy controls, and the percentage of Tfh/CD4 T cells in HT patients is positively correlated with TPOAb,TGAb level. The percentage of Th17/CD4 T cells was positively correlated with the level of TPOAb. However, the percentage of Treg/CD4 T cells in peripheral blood of HT patients and GD patients was significantly lower than that of healthy controls, and the percentage of Treg/CD4 T cells was negatively correlated with TPOAb. There was no statistical significance between the percentage of Treg/CD4 T cells and TPOAb. The relationship between Treg cells and TPOAb,TGAb, which is the most important antibody for the diagnosis of HT, also indicates that the detection of Tfh cells, Th17 cells and Treg cells may be helpful to better understand the pathogenesis of HT.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R581.4
【参考文献】
相关期刊论文 前8条
1 俞敬;何宋兵;朱新国;;滤泡辅助T细胞与肿瘤的研究进展[J];医学研究生学报;2014年04期
2 杨治芳;吴芳;汤佳珍;刘建英;;桥本甲状腺炎患者调节性T细胞的表达及其意义[J];中国全科医学;2013年27期
3 薛海波;马蕾;李元宾;张立;周荣佼;安新荣;;Treg/Th17细胞轴与桥本甲状腺炎自身免疫的相关性[J];中国现代医学杂志;2012年23期
4 宋进展;吴汉妮;钱伟;;慢性淋巴细胞性甲状腺炎患者外周血Th17的检测及意义[J];细胞与分子免疫学杂志;2009年10期
5 王会平;翟志敏;张爱梅;徐修才;李庆;张翠萍;卢娟;沈元元;洪海鸥;;CD4~+CD25~+CD127~(low)识别人外周血CD4~+CD25~+调节性T细胞的优势[J];中国免疫学杂志;2008年12期
6 吴汉妮;余玲;屈新才;钱伟;肖波;;IL-23、IL-17在桥本氏甲状腺炎患者甲状腺组织中的表达[J];细胞与分子免疫学杂志;2008年12期
7 ;中国甲状腺疾病诊治指南——甲状腺功能亢进症[J];中华内科杂志;2007年10期
8 李善;陈广洁;;滤泡辅助性T细胞的分化及其与免疫性疾病的关系[J];国际免疫学杂志;2010年05期
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