滴鼻大肠杆菌对高脂诱导肥胖小鼠糖脂代谢的影响

发布时间：2019-01-12 09:31

【摘要】：目的:探讨滴鼻大肠杆菌对高脂诱导肥胖小鼠糖脂代谢的影响。方法:选取48只高脂诱导的肥胖小鼠作为肥胖组,48只非肥胖小鼠作为对照组,滴鼻40μL含4×10~9 CFUs大肠杆菌菌液,于感染前及感染后1、2、3、4 d检测2组小鼠血清糖脂代谢相关指标及肝脏脂质沉积情况。结果:与感染前比较,肥胖小鼠体质量、附睾脂肪组织质量及指数在感染后1~4d显著降低(P0.05);血清空腹血糖(FBG)、空腹胰岛素(FINS)水平以及胰岛素抵抗指数(HOMA-IR)在2~4d显著升高(P0.05);血清游离脂肪酸(FFA)、甘油三酯(TG)和极低密度脂蛋白(VLDL)水平在1~4 d显著升高总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDLC)水平在1~3d显著降低(P0.05);肝脏质量、指数及TG含量在1~4d显著升高(P0.05),肝细胞脂滴蓄积在感染后2和4d明显增多。与对照组比较肥胖组小鼠血清TC、LDL-C和HDL-C水平在感染后显著降低,其余指标均明显升高(P0.05)。结论:滴鼻大肠杆菌可加剧肥胖小鼠糖脂代谢紊乱程度,促进其胰岛素抵抗及肝脏脂肪化的发生、发展。
[Abstract]:Objective: to investigate the effect of nasal Escherichia coli on glucose and lipid metabolism in obese mice induced by hyperlipidemia. Methods: 48 obese mice induced by hyperlipidemia were selected as obese group and 48 non-obese mice as control group. 40 渭 L nasal drip containing 4 脳 10 9 CFUs Escherichia coli solution was used before and after infection. Serum glucose and lipid metabolism indexes and lipids deposition in liver of the two groups were detected for 4 d. Results: compared with those before infection, the body weight, mass and index of epididymal adipose tissue in obese mice were significantly lower than those before infection (P0.05). The level of fasting insulin (FINS) and insulin resistance index (HOMA-IR) in serum fasting blood glucose (FBG),) and insulin resistance index (HOMA-IR) were significantly increased on the 4th day (P0.05). Serum free fatty acid (FFA), triglyceride (TG) and very low density lipoprotein (VLDL) (VLDL) levels significantly increased total cholesterol (TC), at 1 and 4 d. The levels of low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) (HDLC) decreased significantly at 1 day (P0.05). The liver mass, index and TG content were significantly increased on the 4th day after infection (P0.05), and the lipid droplet accumulation of hepatocytes increased significantly on the 2nd and 4th day after infection. Compared with the control group, the serum TC,LDL-C and HDL-C levels in the obese group decreased significantly after infection, and the other indexes increased significantly (P0.05). Conclusion: nasal Escherichia coli can aggravate the disorder of glucose and lipid metabolism in obese mice and promote the occurrence and development of insulin resistance and hepatic steatosis.
【作者单位】：四川农业大学动物医学院;
【基金】：四川省科技厅支撑项目(No.2013NZ0032)
【分类号】：R589.2

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