载辛伐他汀硫酸钙对去卵巢大鼠胫骨骨缺损修复的影响
发布时间：2019-01-24 20:40
【摘要】：目的观察辛伐他汀与硫酸钙混合后对骨质疏松骨缺损修复的促进作用。方法选用健康3月龄雌性SD大鼠48只,切除双侧卵巢,低钙饮食3月,并将胫骨上端造成3 mm骨缺损,随机均分为3组,未植骨组直接缝合皮肤,硫酸钙组在骨缺损处植入硫酸钙,载辛伐他汀硫酸钙组植入载辛伐他汀硫酸钙,植入后4、8周取标本进行组织学观察骨痂形成情况,植入后8周行Micro-CT检测,于12周对标本进行生物力学分析。结果植入4、8周时,载辛伐他汀硫酸钙组骨愈合程度较硫酸钙组和未植骨组有显著性改善,8周时,Micro-CT检测骨缺损区内骨体积分数(BV/TV),组织骨密度(t BMD),骨小梁数量(Tb.N),载辛伐他汀硫酸钙组优于后两组,12周时生物力学检测,载辛伐他汀硫酸钙组失效强度和刚度优于硫酸钙组和未植骨组(P0.05)。结论载辛伐他汀硫酸钙对骨质疏松性骨缺损具有良好的成骨和骨诱导作用,可以较好地促进骨缺损的修复。
[Abstract]:Objective to observe the promoting effect of simvastatin and calcium sulfate on the repair of osteoporosis bone defect. Methods Forty-eight healthy 3-month-old female SD rats were randomly divided into 3 groups, with bilateral ovaries resected and low-calcium diet for 3 months, and 3 mm bone defects were caused at the upper end of the tibia, and the skin was sutured directly in the ungrafted group. Calcium sulfate was implanted into bone defect in calcium sulfate group, and calcium sulfate was implanted in simvastatin sulfate group. Histological observation of callus formation was performed 8 weeks after implantation, and Micro-CT was detected 8 weeks after implantation. Biomechanical analysis of the specimens was carried out at 12 weeks. Results at the 8th week of implantation, the healing degree of bone in the calcium sulfate group was significantly improved than that in the calcium sulfate group and the ungrafted group. At 8 weeks, bone volume fraction (BV/TV) and bone density (t BMD),) in the bone defect area were measured by Micro-CT. The number of trabeculae (Tb.N) and the number of trabeculae (Tb.N) in the calcium sulfate group were better than those in the latter two groups. At 12 weeks, the failure strength and stiffness of the calcium sulfate group were better than those of the calcium sulfate group and the ungrafted group (P0.05). Conclusion calcium simvastatin sulfate has good osteogenic and bone-inducing effects on osteoporotic bone defect and can promote the repair of bone defect.
【作者单位】： 南京医科大学附属苏州医院关节外科;苏州大学附属第二医院关节外科;
【基金】：苏州市科教兴卫发展项目(KJXW2013024)
【分类号】：R580;R683
，
本文编号：2414836
[Abstract]:Objective to observe the promoting effect of simvastatin and calcium sulfate on the repair of osteoporosis bone defect. Methods Forty-eight healthy 3-month-old female SD rats were randomly divided into 3 groups, with bilateral ovaries resected and low-calcium diet for 3 months, and 3 mm bone defects were caused at the upper end of the tibia, and the skin was sutured directly in the ungrafted group. Calcium sulfate was implanted into bone defect in calcium sulfate group, and calcium sulfate was implanted in simvastatin sulfate group. Histological observation of callus formation was performed 8 weeks after implantation, and Micro-CT was detected 8 weeks after implantation. Biomechanical analysis of the specimens was carried out at 12 weeks. Results at the 8th week of implantation, the healing degree of bone in the calcium sulfate group was significantly improved than that in the calcium sulfate group and the ungrafted group. At 8 weeks, bone volume fraction (BV/TV) and bone density (t BMD),) in the bone defect area were measured by Micro-CT. The number of trabeculae (Tb.N) and the number of trabeculae (Tb.N) in the calcium sulfate group were better than those in the latter two groups. At 12 weeks, the failure strength and stiffness of the calcium sulfate group were better than those of the calcium sulfate group and the ungrafted group (P0.05). Conclusion calcium simvastatin sulfate has good osteogenic and bone-inducing effects on osteoporotic bone defect and can promote the repair of bone defect.
【作者单位】： 南京医科大学附属苏州医院关节外科;苏州大学附属第二医院关节外科;
【基金】：苏州市科教兴卫发展项目(KJXW2013024)
【分类号】：R580;R683
，
本文编号：2414836
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