二甲双胍对结肠癌干细胞增殖、凋亡及CD133表达影响的研究

发布时间：2019-01-25 08:05

【摘要】：目的探讨二甲双胍(MET)对结肠癌干细胞增殖、凋亡及CD133表达的影响。方法免疫磁珠法从结肠癌细胞株SW480中分选出结肠癌干细胞,流式细胞仪检测其表面标志物CD133。不同浓度MET干预结肠癌干细胞后,CCK-8检测24、48 h细胞增殖;流式细胞术检测细胞凋亡;Western blot检测CD133蛋白表达。结果不同浓度(1、5、10、20 mmol/L)MET干预后,24 h细胞活力[(89.3±16.5)%vs(61.6±16.8)%vs(45.1±14.7)%vs(35.4±13.7)%]与48 h细胞活力[(68.9±25.5)%vs(38.1±17.4)%vs(23.3±6.5)%vs(17.8±6.3)%]比较,差异有统计学意义(P0.01);与对照组比较,MET(20 mmol/L)干预后的细胞晚期凋亡率(13.5±3.3)%及总凋亡率(23.6±4.9)%差异有统计学意义(P0.05或P0.01);MET(10、20 mmol/L)干预后CD133蛋白表达分别为(0.80±0.23)和(0.33±0.19),与对照组比较差异有统计学意义(P0.05)。结论 MET抑制结肠癌干细胞增殖、促进其凋亡,减少其表面标志物CD133蛋白表达。
[Abstract]:Objective to investigate the effects of metformin (MET) on the proliferation, apoptosis and CD133 expression of colon cancer stem cells. Methods Colon cancer stem cells were isolated from colon cancer cell line SW480 by immunomagnetic bead method. The surface marker CD133. was detected by flow cytometry. The proliferation of colon cancer stem cells was detected by CCK-8, and the expression of CD133 protein was detected by flow cytometry, apoptosis; Western blot was detected by flow cytometry. Results after the intervention of MET with different concentrations (1 / 10 ~ 10 ~ (20) mmol/L), 24 h cell viability [(89.3 卤16.5)% vs (61.6 卤16.8)% vs (45.1 卤14.7)% vs (35.4 卤13.7)%] and 48h cell viability [(68.9 卤25.5)% vs (38.1 卤17.4)% vs]. 23.3 卤6.5% vs (17.8 卤6.3%). The difference was statistically significant (P0.01). Compared with the control group, the late apoptosis rate (13.5 卤3.3)% and the total apoptosis rate (23.6 卤4.9)% after, MET (20 mmol/L intervention were significantly different (P0.05 or P0.01). The expression of CD133 protein was (0.80 卤0.23) and (0.33 卤0.19) after the intervention of MET (10 ~ 20 mmol/L), which was significantly different from that of the control group (P0.05). Conclusion MET inhibits the proliferation of colon cancer stem cells, promotes their apoptosis and reduces the expression of CD133 protein.
【作者单位】：安徽医科大学第一附属医院内分泌科;
【基金】：安徽省自然科学基金(1508085MH150)
【分类号】：R587.1;R735.35

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