Treg参与亚急性甲状腺炎发病免疫机制的初步探讨
发布时间：2019-02-18 16:37
【摘要】：目的:通过检测亚急性甲状腺炎(SAT)患者外周血中调节性T细胞(Treg)的比例及相关细胞因子的浓度;检测SAT患者甲状腺组织中叉头蛋白3(Foxp3)的表达及分布情况,初步探讨Treg细胞在SAT免疫发病机制中的作用和可能的机制。方法:选取46名SAT患者及15名正常对照者,用流式细胞术检测外周血中CD4+CD25+T细胞、CD4+CD25+CD127-Treg细胞占CD4+T细胞的比例;用ELISA法检测血清中白细胞介素-10(IL-10)、转化生长因子β1(TGF-β1)及前列腺素E2(PGE2)的浓度;同时检测甲状腺功能、血沉(ESR)及C反应蛋白(CRP)等一般生化指标;将SAT组与正常对照组的上述指标进行比较,并进行各指标的相关性分析。另选取29名SAT患者及20名正常对照者的甲状腺标本,用免疫组织化学方法检测甲状腺组织中Foxp3的表达情况,并进行两组间的比较。结果:1.一般情况:与正常对照组比较,SAT组TT3、TT4、FT3、FT4和CRP浓度升高,TSH浓度降低,ESR速度加快,差异均具有统计学意义(P0.05);2.外周血Treg细胞比例:SAT组外周血CD4+CD25+CD127-Treg细胞比例明显低于正常对照组,差异有统计学意义(P0.05),而CD4+CD25+T细胞比例及CD4+T细胞比例与正常对照组比较差异无统计学意义(P0.05);3.血清中细胞因子的浓度:SAT组血清TGF-β1浓度明显高于正常对照组(P0.05),IL-10及PGE2浓度与正常对照组比较差异无统计学意义(P0.05);4.甲状腺组织中Foxp3蛋白的表达:SAT组甲状腺组织中Foxp3表达阳性率明显高于正常对照组,差异有统计学意义(P0.05);5.相关性分析:SAT患者TGF-β1浓度与TT3、TT4、TSH、ESR及CRP均无明显相关性(P0.05),Treg细胞比例与TT3、TT4、FT3、FT4、TSH、ESR及CRP均无明显相关性(P0.05),ESR与CRP呈正相关(r=0.654,P0.05)。结论:1.SAT患者外周血中Treg细胞比例下降,而局部甲状腺组织中Foxp3蛋白的表达量却增高,Treg细胞的总体下降和甲状腺组织的炎症损伤加剧是导致SAT发生的原因。局部甲状腺组织中Treg细胞浸润增加可能是机体保护性调节作用的结果;2.Treg细胞的分化及成熟异常与SAT发病有关,但与SAT的炎症强度、临床表现和甲状腺功能等指标相关性不强,因此Treg细胞的分化及成熟异常与病情的轻重无关。
[Abstract]:Objective: to detect the proportion of regulatory T cell (Treg) and the concentration of cytokines in peripheral blood of patients with subacute thyroiditis (SAT). The expression and distribution of fork head protein 3 (Foxp3) in thyroid gland of patients with SAT were detected, and the role and possible mechanism of Treg cells in the pathogenesis of SAT were preliminarily investigated. Methods: 46 patients with SAT and 15 normal controls were selected to detect the percentage of CD4 CD25 T cells in peripheral blood by flow cytometry, and the proportion of CD4 CD25 CD127-Treg cells to CD4 T cells was determined by flow cytometry. The concentrations of interleukin-10 (IL-10), transforming growth factor 尾 1 (TGF- 尾 1) and prostaglandin E 2 (PGE2) in serum were detected by ELISA method. Thyroid function, erythrocyte sedimentation rate (ESR), (ESR) and C-reactive protein (CRP) were detected and compared between SAT group and normal control group. The expression of Foxp3 in thyroid tissue of 29 patients with SAT and 20 normal controls was detected by immunohistochemical method and compared between the two groups. Results: 1. General conditions: compared with the normal control group, SAT group TT3,TT4,FT3,FT4 and CRP concentration increased, TSH concentration decreased, ESR speed up, the differences were statistically significant (P0.05); 2. Proportion of peripheral blood Treg cells: the proportion of peripheral blood CD4 CD25 CD127-Treg cells in SAT group was significantly lower than that in normal control group, the difference was statistically significant (P0.05). The ratio of CD4 CD25 T cells and CD4 T cells was not significantly different from that of normal control group (P0.05). 3. Serum cytokine concentration: serum TGF- 尾 1 concentration in SAT group was significantly higher than that in normal control group (P0.05), IL-10 and PGE2 levels were not significantly different from those in normal control group (P0.05). The expression of Foxp3 protein in thyroid tissue: the positive rate of Foxp3 expression in thyroid tissue of SAT group was significantly higher than that of normal control group (P0.05). Correlation analysis: there was no significant correlation between TGF- 尾 1 concentration and TT3,TT4,TSH,ESR and CRP in SAT patients (P0.05). There was no significant correlation between), Treg cell ratio and TT3,TT4,FT3,FT4,TSH,ESR and CRP (P0.05). There was a positive correlation between ESR and CRP (P 0.05). Conclusion: the proportion of Treg cells in peripheral blood of patients with 1.SAT decreased, but the expression of Foxp3 protein increased in local thyroid tissue. The overall decrease of Treg cells and the exacerbation of inflammation in thyroid tissue were the causes of SAT. The increase of Treg cell infiltration in local thyroid tissue may be the result of protective regulation. The abnormal differentiation and maturation of 2.Treg cells were related to the pathogenesis of SAT, but not to the inflammation intensity, clinical manifestation and thyroid function of SAT. Therefore, the abnormal differentiation and maturation of Treg cells were not related to the severity of the disease.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R581.4
本文编号：2426028
[Abstract]:Objective: to detect the proportion of regulatory T cell (Treg) and the concentration of cytokines in peripheral blood of patients with subacute thyroiditis (SAT). The expression and distribution of fork head protein 3 (Foxp3) in thyroid gland of patients with SAT were detected, and the role and possible mechanism of Treg cells in the pathogenesis of SAT were preliminarily investigated. Methods: 46 patients with SAT and 15 normal controls were selected to detect the percentage of CD4 CD25 T cells in peripheral blood by flow cytometry, and the proportion of CD4 CD25 CD127-Treg cells to CD4 T cells was determined by flow cytometry. The concentrations of interleukin-10 (IL-10), transforming growth factor 尾 1 (TGF- 尾 1) and prostaglandin E 2 (PGE2) in serum were detected by ELISA method. Thyroid function, erythrocyte sedimentation rate (ESR), (ESR) and C-reactive protein (CRP) were detected and compared between SAT group and normal control group. The expression of Foxp3 in thyroid tissue of 29 patients with SAT and 20 normal controls was detected by immunohistochemical method and compared between the two groups. Results: 1. General conditions: compared with the normal control group, SAT group TT3,TT4,FT3,FT4 and CRP concentration increased, TSH concentration decreased, ESR speed up, the differences were statistically significant (P0.05); 2. Proportion of peripheral blood Treg cells: the proportion of peripheral blood CD4 CD25 CD127-Treg cells in SAT group was significantly lower than that in normal control group, the difference was statistically significant (P0.05). The ratio of CD4 CD25 T cells and CD4 T cells was not significantly different from that of normal control group (P0.05). 3. Serum cytokine concentration: serum TGF- 尾 1 concentration in SAT group was significantly higher than that in normal control group (P0.05), IL-10 and PGE2 levels were not significantly different from those in normal control group (P0.05). The expression of Foxp3 protein in thyroid tissue: the positive rate of Foxp3 expression in thyroid tissue of SAT group was significantly higher than that of normal control group (P0.05). Correlation analysis: there was no significant correlation between TGF- 尾 1 concentration and TT3,TT4,TSH,ESR and CRP in SAT patients (P0.05). There was no significant correlation between), Treg cell ratio and TT3,TT4,FT3,FT4,TSH,ESR and CRP (P0.05). There was a positive correlation between ESR and CRP (P 0.05). Conclusion: the proportion of Treg cells in peripheral blood of patients with 1.SAT decreased, but the expression of Foxp3 protein increased in local thyroid tissue. The overall decrease of Treg cells and the exacerbation of inflammation in thyroid tissue were the causes of SAT. The increase of Treg cell infiltration in local thyroid tissue may be the result of protective regulation. The abnormal differentiation and maturation of 2.Treg cells were related to the pathogenesis of SAT, but not to the inflammation intensity, clinical manifestation and thyroid function of SAT. Therefore, the abnormal differentiation and maturation of Treg cells were not related to the severity of the disease.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R581.4
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