普洱熟茶熏蒸对营养性肥胖大鼠减肥作用及肠道菌群的研究
发布时间：2019-02-25 15:53
【摘要】：随着人们生活方式的变化,肥胖已经成为全球范围内的流行病,因肥胖导致的糖尿病、高血压、冠心病、高脂血症等严重影响着人类健康,肥胖症被世界卫生组织列为与癌症并列的21世纪威胁人类健康最严重的疾病,因此对肥胖症的研究已成为重大的现代医学课题。肠道微生物能影响动物的健康状况,研究表明食物、药物等至少部分通过影响肠道微生物而起作用,特别是肥胖。但外环境的变化是否也会导致肠道微生物从而影响身体健康状况尚未有明确的结论。我们之前创新性的研究表明,茶叶水熏蒸对高脂饲料诱导的营养型肥胖大鼠有良好减肥效果,提示茶水熏蒸这一改变外部环境的方式很可能也会影响体内肠道微生物。本研究采用高脂饲料饲喂大鼠,构建营养性肥胖模型大鼠,采用普洱熟茶熏蒸给予温度(39±1)℃浓度6.67%的茶汤进行45天的熏蒸实验,并采用试剂盒提取大鼠粪便微生物的总DNA,通过Illumina高通量测序技术对16SrRNA基因的V6可变区进行测序分析,再结合实验动物体重以及血液TC、TG、HDL-C、LDL-C、ALT和AST活性等生理生化指标、肝脏切片等数据,来探讨肥胖大鼠在普洱熟茶熏蒸减肥过程中肠道微生物的多样性差异。本实验结果表明,普洱熟茶熏蒸对营养性肥胖大鼠有一定的减重作用,而且可以降低血清TC、TG、LDL-C、血糖含量和ALT、AST酶活性,升高血清HDL-C含量,从肝脏病理组织的切片观察发现,普洱熟茶熏蒸可以缓解由于高脂饮食引起的肝脏损伤。此外,大鼠在构建肥胖模型阶段时大鼠的肠道菌群多样性相比其他阶段显著丰富,当大鼠进入减肥阶段后,在减肥过程中大鼠的肠道菌群发生了改变,一些在建模阶段丰度高的菌群开始降低,而一些在建模阶段丰度低的菌群开始升高。茶熏使得厚壁菌增多,拟杆菌减少,而停止茶熏后厚壁菌丰度降低了。本项目还对开拓产能已经过剩的茶叶的新用途、预防和治疗肥胖提供新的思路。
[Abstract]:With the change of people's lifestyle, obesity has become a global epidemic. Diabetes, hypertension, coronary heart disease and hyperlipidemia caused by obesity seriously affect human health. Obesity is listed by WHO as the most serious disease threatening human health in the 21st century, which is side by side with cancer. Therefore, the study of obesity has become a major subject of modern medicine. Intestinal microorganisms can affect the health of animals. Studies have shown that food, drugs and so on play a role in at least part of the effects of intestinal microorganisms, especially obesity. However, whether changes in the external environment will also lead to intestinal microbe and affect the health of the body has not been clearly concluded. Our previous innovative studies have shown that tea water fumigation has a good weight loss effect on nutritious obese rats induced by high-fat diet, suggesting that tea water fumigation, a way to change the external environment, may also affect intestinal microbe in vivo. In this study, the rats were fed with high-fat diet to establish the model of nutritional obesity. The fumigation experiment of Puer mature tea with 6.67% tea soup at (39 卤1) 鈩，
本文编号：2430328
[Abstract]:With the change of people's lifestyle, obesity has become a global epidemic. Diabetes, hypertension, coronary heart disease and hyperlipidemia caused by obesity seriously affect human health. Obesity is listed by WHO as the most serious disease threatening human health in the 21st century, which is side by side with cancer. Therefore, the study of obesity has become a major subject of modern medicine. Intestinal microorganisms can affect the health of animals. Studies have shown that food, drugs and so on play a role in at least part of the effects of intestinal microorganisms, especially obesity. However, whether changes in the external environment will also lead to intestinal microbe and affect the health of the body has not been clearly concluded. Our previous innovative studies have shown that tea water fumigation has a good weight loss effect on nutritious obese rats induced by high-fat diet, suggesting that tea water fumigation, a way to change the external environment, may also affect intestinal microbe in vivo. In this study, the rats were fed with high-fat diet to establish the model of nutritional obesity. The fumigation experiment of Puer mature tea with 6.67% tea soup at (39 卤1) 鈩，
本文编号：2430328
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