牛磺酸对百草枯中毒大鼠肾损伤的作用及机制研究

发布时间：2019-03-19 18:34

【摘要】：目的:探讨不同剂量牛磺酸对百草枯中毒大鼠肾脏氧化应激和炎症反应的影响。方法:选取48只雄性SD大鼠随机分为阴性对照组、百草枯染毒组、百草枯染毒+小剂量牛磺酸组和百草枯染毒+大剂量牛磺酸组。采用生化检测仪检测大鼠血清肌酐及尿素氮水平;比色法检测血标本中丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)水平评估氧化应激状态;另以ELISA法检测血标本中IL-6及细胞间黏附分子1(ICAM-1)水平评估炎症反应;DHE荧光探针检测肾脏活性氧簇(reactive oxygen species,ROS)水平;Western blot法检测大鼠肾脏标本丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)中p-P38 MAPK、p-JNK和pERK1/2的蛋白水平;并以real-time PCR法检测大鼠肾脏内TNF-α、TGF-β1及IL-6的mRNA水平。结果:百草枯中毒大鼠血清肌酐及尿素氮增高,牛磺酸干预后降低了中毒大鼠的肌酐及尿素氮水平,且大剂量牛磺酸组的肌酐及尿素氮水平更低。牛磺酸的干预不仅明显减轻了肾脏组织的氧化应激与炎症反应,同时也降低了百草枯中毒大鼠肾脏的MAPK活性。结论:牛磺酸干预能减轻百草枯中毒大鼠的肾损伤,其作用机制可能与下调了肾脏MAPK活性、减轻了肾脏氧化应激及炎症反应有关。
[Abstract]:Aim: to investigate the effects of different doses of taurine on oxidative stress and inflammatory reaction in rats with paraquat poisoning. Methods: 48 male SD rats were randomly divided into negative control group, paraquat low dose taurine group and paraquat high dose taurine group. The levels of serum creatinine and urea nitrogen were measured by biochemical detector, and the levels of malondialdehyde (malondialdehyde,MDA) and superoxide dismutase (superoxide dismutase,SOD) in blood samples were measured by colorimetry to evaluate the state of oxidative stress. The levels of IL-6 and intercellular adhesion molecule-1 (ICAM-1) in blood samples were measured by ELISA, and the levels of active oxygen cluster (reactive oxygen species,ROS (reactive oxygen species,ROS) in kidney were detected by DHE fluorescence probe. The levels of p-P38 MAPK,p-JNK and pERK1/2 in mitogen-activated protein kinase (mitogen-activated protein kinase,MAPK) of rat kidney were detected by Western blot. The mRNA levels of TNF- 伪, TGF- 尾 1 and IL-6 in kidney of rats were measured by real-time PCR method. Results: the serum creatinine and urea nitrogen were increased in paraquat poisoned rats. The levels of creatinine and urea nitrogen were decreased after taurine intervention, and the levels of creatinine and urea nitrogen in high dose taurine group were lower than those in high dose taurine group. Taurine not only reduced the oxidative stress and inflammatory reaction of renal tissue, but also decreased the activity of MAPK in the kidney of paraquat poisoned rats. Conclusion: taurine can relieve renal injury induced by paraquat poisoning in rats, and its mechanism may be related to the down-regulation of renal MAPK activity and the reduction of oxidative stress and inflammatory reaction of kidney.
【作者单位】：中国医科大学附属盛京医院急诊科;中国医科大学附属盛京医院内分泌科;
【基金】：国家自然科学基金面上项目(No.81671898) 辽宁省教育厅项目(No.LK201603;No.LK201633)
【分类号】：R595.4

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