阿格列汀对糖尿病大鼠肾脏病理改变的影响

发布时间：2019-03-25 13:42

【摘要】：目的探讨糖尿病(DM)大鼠肾病中肾脏病理改变、细胞凋亡的机制及阿格列汀对细胞凋亡的影响。方法 8周龄雄性Wistar大鼠50只,随机分为对照组(n=10),DM组(n=20),阿格列汀组(n=20)。各组正常饮食,腹腔注射小剂量链脲佐菌素构建糖尿病模型。模型稳定后,给予对照组、糖尿病组生理盐水灌胃,阿格列汀组以一定剂量阿格列汀研钵磨碎后生理盐水稀释灌胃。于21 w末监测血糖、血肌酐、尿素氮等生化指标,21 w末处死大鼠后苏木素-伊红(HE)染色、PAS染色、Masson染色等病理检测肾功能及损伤情况;免疫组化、Western印迹检测肾组织受体相互作用蛋白(RIP)3、Caspase-3、聚腺苷二磷酸-核糖聚合酶(PARP)的表达情况。结果与对照组相比,糖尿病组系膜细胞基底膜增厚,细胞外基质堆积,RIP3、Caspase-3、PARP表达增多,阿格列汀组肾脏病理损伤较轻,相应表达减少,但比对照组稍多,Western印迹结果阿格列汀组相对对照组变化不大。结论阿格列汀可能通过改善细胞凋亡通路中的死亡受体通路中的RIP3的表达改善肾脏细胞的凋亡程度,延缓肾脏病理损伤的进展。
[Abstract]:Objective to investigate the pathological changes of kidney and the mechanism of apoptosis in diabetic (DM) rats and the effect of acitriptyline on apoptosis. Methods Fifty 8-week-old male Wistar rats were randomly divided into two groups: control group (n = 10), DM, n = 20) and aglitine group (n = 20). The diabetic model was established by intraperitoneal injection of low dose streptozotocin. After the model was stable, normal saline was given to the control group, the diabetic group was administered intragastrically with saline, and the Asiglitine group was diluted with a certain dose of Aglutine mortar after being ground up. Biochemical indexes such as blood glucose, serum creatinine and urea nitrogen were monitored at the end of the 21st week. Renal function and injury were detected by hematoxylin-eosin (HE) staining, PAS staining and Masson staining after the rats were killed at the end of the 21st week. Immunohistochemistry and Western blot were used to detect the expression of renal receptor interacting protein (RIP) 3, caspase 3 and poly (adenosine diphosphate) ribose polymerase (PARP). Results compared with the control group, the Mesangial cell basement membrane thickened, extracellular matrix accumulation and RIP3,Caspase-3,PARP expression increased in the diabetic group. The renal pathological injury was mild and the corresponding expression was decreased in the Aglitine group, but a little more than that in the control group. The results of Western blot showed that there was no significant change in the aglitine group compared with the control group. Conclusion Asiglitine may improve the apoptosis of renal cells by improving the expression of RIP3 in the death receptor pathway of apoptosis pathway, and delay the progression of renal pathological injury.
【作者单位】：山东大学第二医院;
【基金】：山东省科技发展计划项目(No.2013G0021813) 山东省中医药管理局(No.2011-029) 济南市高校自主创新计划(No.201401218) 中国心血管医师研究基金(2014)
【分类号】：R587.2;R692.9

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