系统性红斑狼疮患者外周血单核细胞PD-L2分子的表达及其临床意义

发布时间：2019-04-17 10:06

【摘要】：目的:分析系统性红斑狼疮患者外周血单核细胞PD-L2分子的表达及其与疾病活动程度的相关性。方法:收集26例系统性红斑狼疮患者及38例健康对照组外周血,Ficoll分离单个核细胞后,TRIZOL抽提总RNA,定量PCR分析PD-L2基因的表达,流式细胞仪检测CD14、PD-L2分子的表达。统计学分析其相关性及与疾病活动程度之间的关联。结果:定量PCR结果提示系统性红斑狼疮患者外周血单核细胞PD-L2基因表达高于健康对照组(P0.05),流式发现单核细胞上PD-L2高表达(P0.05),且活动期患者表达高于稳定期,并且与疾病的评分(SLEDAI)呈正相关(P0.05)。结论:免疫分子PD-L2可能参与系统性红斑狼疮疾病的进程并可能成为疾病预防及治疗的潜在分子。
[Abstract]:Aim: to investigate the expression of PD-L2 molecule in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE) and its correlation with disease activity. Methods: peripheral blood samples were collected from 26 patients with systemic lupus erythematosus (SLE) and 38 healthy controls. Mononuclear cells were isolated from Ficoll. The expression of PD-L2 gene was analyzed by quantitative PCR of total RNA, extracted from TRIZOL, and the expression of CD14,PD-L2 molecule was detected by flow cytometry. Statistical analysis of its correlation and the degree of disease activity. Results: the results of quantitative PCR showed that the expression of PD-L2 gene in peripheral blood mononuclear cells of patients with systemic lupus erythematosus was higher than that of healthy controls (P0.05), and the high expression of PD-L2 on monocytes was found by flow cytometry (P0.05). The expression in active stage was higher than that in stable stage, and was positively correlated with the disease score (SLEDAI) (P0.05). Conclusion: immune molecule PD-L2 may be involved in the process of systemic lupus erythematosus disease and may become a potential molecule for disease prevention and treatment.
【作者单位】：苏州卫生职业技术学院苏州市检验医学重点实验室;苏州科技城医院;苏州大学附属第一医院临床免疫研究所;
【基金】：国家自然科学基金资助项目(31170836) 江苏省高校优秀科技创新团队项目(自身免疫病抗体的挖掘和应用)的资助
【分类号】：R593.241

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