Ghrelin对糖尿病大鼠心肌动作电位和瞬时外向钾电流的影响

发布时间：2019-06-03 09:11

【摘要】：目的探讨外源性ghrelin对糖尿病心肌病(DCM)大鼠心肌动作电位及瞬时外向钾电流(Ito)的影响。方法雄性SD大鼠腹腔注射链脲佐菌素(60mg/kg)诱导Ⅰ型DCM模型,并采用全细胞膜片钳技术观察ghrelin对糖尿病大鼠心室肌细胞动作电位及瞬时外向钾电流的影响。结果与DCM组比较,10~(-7) mmol/L ghrelin明显延长DCM大鼠心室肌细胞5%动作电位时程(APD50)[(12.49±2.32)%;n=7,P=0.037]及APD90[(26.29±5.13)%;n=7,P=0.006];并明显抑制Ito电流峰值,抑制率[(23.14±3.07)%;n=9,P=0.021]。结论外源性ghrelin抑制Ito电流而延长DCM大鼠心肌动作电位时程,参与糖尿病大鼠心脏电生理的重构。
[Abstract]:Objective to investigate the effect of exogenous ghrelin on myocardial action potential and transient outward potassium current (Ito) in diabetic cardiomyopathy (DCM) rats. Methods Type I DCM model was induced by intraabdominal injection of streptozotocin (60mg/kg) in male SD rats. The effects of ghrelin on action potential and transient outward potassium current in ventricular myocytes of diabetic rats were observed by whole cell patch clamp technique. Results compared with DCM group, 10 ~ (- 7) mmol/L ghrelin significantly prolonged 5% action potential duration (APD50) of ventricular myocytes in DCM rats [(12.49 卤2.32)%; n 鈮，

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