ω-3多不饱和脂肪酸对去势大鼠骨微结构的影响

发布时间：2019-06-14 09:13

【摘要】：目的:观察ω-3多不饱和脂肪酸对去势大鼠骨微结构的影响。方法:将30只雌性大鼠随机分为假手术组、模型组和试验组。假手术组仅切除卵巢周围脂肪组织,其余两组切除双侧卵巢。模型组和假手术组给1 m L/(100 g·d)的生理盐水灌胃,试验组给予ω-3 PUFA 150 mg/(kg·d)。干预12周后,处死所有大鼠,检测血钙、磷、碱性磷酸酶和雌激素水平。分离右侧整段股骨应用micro-CT检测骨密度和骨微结构。结果:雌二醇在3组之间存在显著差异,表现为模型组水平最低,而假手术组水平最高。试验组松质骨骨密度、BV/TV、Tb.Th和Tb.N均显著高于模型组,而Tb.Sp显著低于模型组(P0.05);试验组皮质骨骨密度、BV/TV和Cr.Th均显著高于模型组,而孔隙率显著低于模型组(P0.05)。结论:ω-3 PUFA对去卵巢大鼠的骨微结构有一定的改善作用,这对于防治骨质疏松具有一定的意义。
[Abstract]:Objective: To observe the effect of 1-3 polyunsaturated fatty acid on the bone microstructure of castrated rats. Methods:30 female rats were randomly divided into sham operation group, model group and test group. The sham-operated group only cut off the adipose tissue around the ovary and the remaining two groups cut off the bilateral ovaries. The model group and the sham operation group were given intragastric administration of 1 m L/ (100 g 路 d), and the test group was given 1-3 PUFA 150 mg/ (kg 路 d). After 12 weeks of intervention, all rats were sacrificed and blood calcium, phosphorus, alkaline phosphatase, and estrogen levels were detected. The bone mineral density and bone microstructure were detected by micro-CT in the right whole femur. Results: There was a significant difference between the three groups, and the level of the model group was the lowest, while the level of the sham operation group was the highest. The bone mineral density, BV/ TV, Tb. Th and Tb. N in the test group were significantly higher than that of the model group (P0.05). The bone mineral density, BV/ TV and Cr. Th in the cortical bone of the test group were significantly higher than that of the model group (P0.05). Conclusion: The effect of 3-3 PUFA on the bone microstructure of ovariectomized rats is of great significance to the prevention and treatment of osteoporosis.
【作者单位】：河北省保定市第二医院核医学科;浙江省温州市中心医院外科;
【分类号】：R580

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