血清鼠科肉瘤病毒癌基因同源物B1蛋白的检测与类风湿关节炎
发布时间：2019-06-20 23:43
【摘要】：目的:检测类风湿关节炎(rheumatoid arthritis,RA)患者血清中鼠科肉瘤病毒癌基因同源物B1(v-raf murine sarcoma viral oncogene homologue B1,BRAF)蛋白水平,探讨该蛋白在RA诊断中的意义。方法:纳入RA患者78例、其他风湿病患者96例、健康对照30例,应用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测所有患者血清中BRAF蛋白水平,分析BRAF蛋白与RA患者临床与实验室指标的关系。结果:RA患者血清中BRAF蛋白水平明显高于其他风湿病组(P0.01)及健康对照组(P0.01),BRAF蛋白与类风湿因子(rheumatoid factor,RF)及免疫球蛋白A(immunoglobulin A,Ig A)水平呈正相关(P0.01),与其他临床、实验室指标及细胞因子无相关性。将RA患者分为BRAF蛋白升高组及正常组,比较两组之间的差异,结果显示两组在临床及实验室指标分布上差异均无统计学意义。结论:RA患者血清中BRAF蛋白水平显著高于其他疾病组及健康对照组,提示BRAF蛋白可能参与RA的发病过程。
[Abstract]:Aim: to detect the level of mouse sarcomas virus homologue B1 (v-raf murine sarcoma viral oncogene homologue B1, BRAF) protein in serum of patients with rheumatoid arthritis (rheumatoid arthritis,RA), and to explore the significance of BRAF protein in the diagnosis of RA. Methods: 78 patients with RA, 96 patients with other rheumatism and 30 healthy controls were enrolled in this study. Serum BRAF protein levels were detected by enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay,ELISA). The relationship between BRAF protein and clinical and laboratory indexes of RA patients was analyzed. Results: the level of BRAF protein in serum of patients with RA was significantly higher than that of other rheumatism groups (P 0.01) and healthy control group (P 0.01). There was a positive correlation between), BRAF protein and rheumatoid factor (rheumatoid factor,RF) and immunoglobulin A (immunoglobulin A, GA). There was no correlation with other clinical, laboratory indexes and cytokines. The patients with RA were divided into BRAF protein elevation group and normal group, and the differences between the two groups were compared. the results showed that there was no significant difference in the distribution of clinical and laboratory indexes between the two groups. Conclusion: the level of BRAF protein in serum of patients with RA is significantly higher than that of other diseases and healthy controls, suggesting that BRAF protein may be involved in the pathogenesis of RA.
【作者单位】： 北京大学第三医院风湿免疫科;
【基金】：国家自然科学基金(81273293) 北京大学第三医院院种子基金(Y87473-01)资助~~
【分类号】：R593.22
，
本文编号：2503612
[Abstract]:Aim: to detect the level of mouse sarcomas virus homologue B1 (v-raf murine sarcoma viral oncogene homologue B1, BRAF) protein in serum of patients with rheumatoid arthritis (rheumatoid arthritis,RA), and to explore the significance of BRAF protein in the diagnosis of RA. Methods: 78 patients with RA, 96 patients with other rheumatism and 30 healthy controls were enrolled in this study. Serum BRAF protein levels were detected by enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay,ELISA). The relationship between BRAF protein and clinical and laboratory indexes of RA patients was analyzed. Results: the level of BRAF protein in serum of patients with RA was significantly higher than that of other rheumatism groups (P 0.01) and healthy control group (P 0.01). There was a positive correlation between), BRAF protein and rheumatoid factor (rheumatoid factor,RF) and immunoglobulin A (immunoglobulin A, GA). There was no correlation with other clinical, laboratory indexes and cytokines. The patients with RA were divided into BRAF protein elevation group and normal group, and the differences between the two groups were compared. the results showed that there was no significant difference in the distribution of clinical and laboratory indexes between the two groups. Conclusion: the level of BRAF protein in serum of patients with RA is significantly higher than that of other diseases and healthy controls, suggesting that BRAF protein may be involved in the pathogenesis of RA.
【作者单位】： 北京大学第三医院风湿免疫科;
【基金】：国家自然科学基金(81273293) 北京大学第三医院院种子基金(Y87473-01)资助~~
【分类号】：R593.22
，
本文编号：2503612
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