转录因子T-bet/GATA-3对哮喘小鼠Th1/Th2调节作用及灭活草分枝杆菌的干预研究
发布时间：2021-01-25 23:55
目的探讨雾化吸入灭活草分枝杆菌对哮喘小鼠T-bet/GATA-3表达的影响,了解草分枝杆菌的免疫调节作用。方法将24只雄性Balb/c小鼠随机分为对照组（A组）、哮喘组（B组）、治疗组（C组）,每组8只。哮喘组和治疗组于第0天、第7天和第14天用25μgOVA和1mg氢氧化铝混合于PBS中的0.2ml混悬液腹腔注射小鼠,第21至27天用2%OVA·PBS雾化激发小鼠,每天30min,治疗组在第28天至第32天雾化吸入1支灭活草分枝杆菌稀释于10ml的生理盐水中,每日1次。对照组则用生理盐水代替腹腔注射和雾化激发,哮喘组用生理盐水代替灭活草分枝杆菌雾化治疗,雾化过程中观察小鼠的行为及活动情况等变化。最后一次雾化12h内测小鼠气道反应性,24h内将小鼠处死,支气管肺泡灌洗液（BALF）,左肺组织病理切片行HE染色和PAS染色观察小鼠肺内炎症改变,参照文献方法行病理半定量评分,明确各组小鼠气道炎症严重程度;肺组织病理切片行免疫组化观察T-bet和GATA-3的表达量;ELISA法检测BALF中IFN-γ和IL-4表达水平;实时荧光定量PCR检测肺组织中T-bet、GATA-3、STAT1、S...
【文章来源】：广西医科大学广西壮族自治区
【文章页数】：89 页
【学位级别】：硕士
【部分图文】：
对照组、哮喘组和治疗组的肺组织Th1和Th2细胞流式细胞散点图
图 2-4 γδT 细胞分选纯度及表达 IFN-γ 及 IL-4 的分Figure2- 4.The purity of γ T cells after magnetic separation isolated from spleen of asthma group mice by using TCR γ the manufacture. γ T+CD3+cells were identified as γ T cellsγ T cells(A) before and (B) after magnetic separation.In sprecoatd with anti-mouse CD3 mAbs(5μg/ml;ebiosciencsupplemented with IL-2(10ng/ml;ebioscience).(C)A repOVA(100μg/ml;sigma),C representative γ T cells with OVAcells were harvested and stained for IFN-γ and IL-4.The expThe representative flow cytometric dot plots of IFN-γ+γ T cecells and IL-4+γ T cells in three group.All data were represmeans SD.The comparisons were determined by one-group;*p<0.05,compared with B group.讨支气管哮喘是一种慢性气道疾病
本文编号：3000110
【文章来源】：广西医科大学广西壮族自治区
【文章页数】：89 页
【学位级别】：硕士
【部分图文】：
对照组、哮喘组和治疗组的肺组织Th1和Th2细胞流式细胞散点图
图 2-4 γδT 细胞分选纯度及表达 IFN-γ 及 IL-4 的分Figure2- 4.The purity of γ T cells after magnetic separation isolated from spleen of asthma group mice by using TCR γ the manufacture. γ T+CD3+cells were identified as γ T cellsγ T cells(A) before and (B) after magnetic separation.In sprecoatd with anti-mouse CD3 mAbs(5μg/ml;ebiosciencsupplemented with IL-2(10ng/ml;ebioscience).(C)A repOVA(100μg/ml;sigma),C representative γ T cells with OVAcells were harvested and stained for IFN-γ and IL-4.The expThe representative flow cytometric dot plots of IFN-γ+γ T cecells and IL-4+γ T cells in three group.All data were represmeans SD.The comparisons were determined by one-group;*p<0.05,compared with B group.讨支气管哮喘是一种慢性气道疾病
本文编号：3000110
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