Rapamycin Protects Against Paraquat-induced Pulmonary Epithe

发布时间：2021-11-05 13:44

　　

【文章来源】： 昆明医科大学云南省

【文章页数】：46 页

【文章目录】：
Abbreviations
Abstract
Ⅰ. Introduction
Ⅱ. Methods
    2.1. Reagents
    2.2. Cell culture and treatment
    2.3. RNA extraction, reverse description (RT) and Real-time PCR assay
        Below is the procedure for Real Time PCR
            Isolating Total RNA, Including miRNA, from Samples
            Reverse Transcription of RNA
            Generating a Standard Curve
            Real-time PCR for Detection of miRNA
    2.4. Western blot analysis
    2.5. Fluorescent immunostaining
    2.6. Statistical analysis
Ⅲ. Results
    3.1 Rapamycin alleviates paraquat-induced EMT in A549 and MRC-5 cells
        3.1.1 The expression level of E-cadherin and α-SMA mRNA in A549 and MRC-5 cells
        3.1.2 The expression level of e-cadherin and alpha-SMA parotein in A549 and MRC-5cells
    3.2 PQ activates the Wnt signaling pathway in A549 and MRC-5 cells,and rapamycin inhibited the these effects of PQ
        3.2.1 PQ activities the Wnt signaling pathway in A549and MRC-5 cells
        3.2.2 Rapamycine inhibited the these effects that PQ activates the Wnt signaling pathwayin A549and MRC-5 cells
    3.3 Activation of Wnt signaling pathway attenuates the inhibitory effects ofrapamycin on the PQ-induced EMT
Ⅳ. Discussion
Ⅴ. In conclusion
Literature review
    1.1. The current opinion of Paraquat induce PF
    1.2. The role of EMT in PQ induce PF
    1.3. The role of Wnt/beta-catenin pathway in PF/PQ induce PF
Bibliography
Published paper
Acknowledgements



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