miRNA调控恶性黑色素瘤细胞上皮-间充质转化的分子网络及机制

发布时间：2018-01-04 20:33

本文关键词：miRNA调控恶性黑色素瘤细胞上皮-间充质转化的分子网络及机制　出处：《遗传》2015年07期 　论文类型：期刊论文

【摘要】：黑色素瘤是一种极易发生转移的恶性皮肤肿瘤,具有高度的致死性。上皮间充质细胞转化(Epithelial-mesenchymal transition,EMT)在胚胎发育过程中起到非常重要的作用,同时在肿瘤的发生和恶化过程中也扮演着重要的角色。mi RNA具有广谱的调节能力,对于肿瘤发生和EMT形成都能产生不同程度的影响。本文整合黑色素瘤细胞系转录组和mi RNA组测序数据,在转录组数据中筛选得到参与肿瘤EMT过程的基因,通过Mirsystem软件预测并从mi RNA组数据中筛选出与之负相关的11个mi RNA,包括mi R-130a-3p、mi R-130b-3p、mi R-125a-5p、mi R-30a-3p、mi R-195-5p、mi R-345-5p、mi R-509-3-5p、mi R-374a-5p、mi R-509-5p、mi R-148a-3p和mi R-330-3p。经过生物信息学分析mi RNA靶基因富集的分子网络和信号途径,发现了两个与细胞发育和细胞间相互作用密切相关的网络,以及多个参与调控EMT过程的信号通路。对11个mi RNA进行分子生物学验证,发现mi R-195-5p、mi R-130a-3p、mi R-509-5p和mi R-509-3-5p共4个可以调节重要肿瘤基因的mi RNA。本研究运用m RNA和mi RNA两种转录组的测序数据筛选EMT相关mi RNA的方法,为肿瘤多组学数据整合分析提供了新的研究思路,并以期能为肿瘤精准基因组学的发展发挥重要的推进作用。
[Abstract]:Melanoma is a malignant skin tumor that can easily metastasize. Epithelial-mesenchymal transition (Epithelial-mesenchymal transition) is highly lethal. EMTs play a very important role in embryonic development, and also play an important role in tumorigenesis and progression. Mi RNA has broad-spectrum regulatory ability. It has different effects on tumorigenesis and EMT formation. This paper integrates the sequence data of melanoma cell line transcriptome and mi RNA group. The genes involved in tumor EMT process were screened from transcriptional data, and 11 mi RNA negatively correlated with these genes were predicted by Mirsystem software and screened from mi RNA group data. Including mi R-130a-3pnmmi R-130b-3pnmmi R-125a-5pnmi R-30a-3pnmmi R-195-5p. Mi R-345-5pN mi R-509-3-5pnmmi R-374a-5pnmmi R-509-5p. Mi R-148a-3p and mi R-330-3p. The molecular network and signal pathway of the enrichment of the target gene of mi RNA were analyzed by bioinformatics. Two networks were found to be closely related to cell development and intercellular interactions, as well as several signaling pathways involved in the regulation of EMT processes. Molecular biological verification of 11 mi RNA was performed. It was found that mi R-195-5png mi R-130a-3p. Mi R-509-5p and mi R-509-3-5p have four genes that can regulate important tumor genes. This study used m RNA and mi. Methods of screening EMT related mi RNA by sequencing data of two transcriptional groups of RNA. It provides a new research idea for the integration and analysis of tumor multi-group data, and hopes to play an important role in promoting the development of tumor precise genomics.
【作者单位】：中国人民解放军总医院皮肤科;解放军第309医院;中国科学院北京基因组研究所中国科学院基因组科学与信息重点实验室;中国科学院大学;
【基金】：国家自然科学基金面上项目(编号:31471236) 国家高技术研究发展计划项目(863计划)(编号:2012AA022502)资助
【分类号】：R739.5
【正文快照】： 1.中国人民解放军总医院皮肤科,北京100853;2.中国科学院北京基因组研究所,中国科学院基因组科学与信息重点实验室,北京100101;3.中国科学院大学,北京100049;4.解放军第309医院,北京100091Molecular networks and mechanisms of epithelial-mesenchymaltransition regulated b

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