1.口服益生菌对儿童特应性皮炎预防作用长期效果的系统评价 2.小鼠皮肤不同毛发周期蛋白质组学及生物信息学的研究

发布时间：2018-01-08 04:14

本文关键词：1.口服益生菌对儿童特应性皮炎预防作用长期效果的系统评价 2.小鼠皮肤不同毛发周期蛋白质组学及生物信息学的研究　出处：《南京医科大学》2016年博士论文　论文类型：学位论文

【摘要】：背景：过敏性疾病在发达国家的发生率逐年增高。特应性皮炎是一常见过敏性疾病,该病自婴儿期发生,反复发作,严重影响患儿的生活质量,加重患儿家长的经济负担。但是目前所有的治疗方法只能缓解其病情发作,而不能避免其反复发作。有研究显示,孕期及出生后早期口服益生菌可以预防特应性皮炎发作,但是对其长期的预防效果报道不多。目的：系统评价口服益生菌对婴幼儿特应性皮炎预防作用的长期效果。方法：计算机检索PubMed、Web of Science、 Cochrane Library、EMBASE、CBM和CNKI数据库,收集益生菌预防婴儿特应性皮炎的随机对照试验,并且评估时间距离服用益生菌的时间不少于5年。检索时限均从建库至2014年7月31日。由2位评价员根据纳入标准和排除标准分别独立筛选文献、提取资料、评价质量并核对后,使用RevMan 5.2软件进行Meta分析。结果：最终纳入6个研究,共1955个研究对象。所有研究均为较高质量的研究。异质性检验显示各项研究具有统计学上的同质性(p=0.10,I2=0.46),用固定效应模型进行Meta分析。结果显示：益生菌组的儿童特应性皮炎发生率低于安慰剂组,两组间差异有统计学意义[RR=0.86,95%CI(0.77-0.96),p=0.005]。而两组间在IgE相关的特应性皮炎及敏感体质的干预作用方面,差异无统计学意义。进一步进行亚组分析,结果显示：母亲在怀孕后期就开始服用益生菌者,儿童在5岁以后的特应性皮炎发生率低于安慰剂组,两组间差异有统计学意义[RR=0.83,95%CI (0.74-0.94), p=0.002]。出生后才开始服用益生菌者,两组间差异无统计学意义(p=0.89)。结论：孕后期至出生后早期服用益生菌可以减低儿童特应性皮炎的发生率,并且这种作用可以维持到将来的五年以上。目的：筛选小鼠不同毛发周期,皮肤中差异表达的蛋白。方法：建立小鼠毛发周期循环的模型,取不同毛发周期(0d,8d,20 d)的皮肤样本,提取蛋白,进行双向电泳分离,通过质谱分析,识别差异表达的蛋白。并运用生物信息学、Gene Ontology对其功能进行初步的分析。结果：有44个差异蛋白通过双向蛋白电泳及质谱分析得到鉴定,选取生长期表达上调的3个蛋白,行Western blotting进行验证。生物信息学发现所得到的差异表达蛋白主要与细胞凋亡、细胞增殖与分化和信号传导等有关。结论：初步筛选出小鼠不同毛发周期,皮肤中存在的44个差异表达的蛋白,可为今后的研究提供方向。
[Abstract]:Background: the incidence of allergic diseases in developed countries increased year by year. Atopic dermatitis is a common allergic disease, the disease from infancy, recurrent, serious impact on the quality of life of the patients, aggravate the economic burden of parents. But now all the treatment methods can only alleviate the disease onset, and can not be avoided the repeated attacks. Studies have shown that prenatal and early postnatal oral probiotics can prevent the onset of atopic dermatitis, but the long-term preventive effect is not much reported. Objective: the long-term effect evaluation system of probiotic atopic dermatitis prevention effect on infants. Methods: We searched PubMed, Web of Science, Cochrane Library EMBASE, CBM, and CNKI database, collection of probiotics in prevention of infantile atopic dermatitis in randomized controlled trials, and to assess the time distance probiotics time not less than 5 years. Were searched from inception to July 31, 2014. By 2 reviewers extracted the data according to the inclusion and exclusion criteria were independently screened literature, quality evaluation and check, use RevMan 5.2 software for Meta analysis. Results: a total of 6 studies, a total of 1955 research objects. All the studies were of high quality. Heterogeneity test showed that the research has statistical homogeneity (p=0.10, I2=0.46), Meta analysis with fixed effect model. The results showed that probiotic group of children with atopic dermatitis incidence was lower than that in the placebo group, the difference between the two groups was statistically significant ([RR=0.86,95%CI 0.77-0.96), p=0.005]. and IgE between the two groups in the atopic dermatitis and allergic intervention, the difference was not statistically significant. Further subgroup analysis, results showed that: the mother began to take probiotics in late pregnancy Those children after the age of 5 atopic dermatitis incidence was lower than that in the placebo group, the difference between the two groups was statistically significant ([RR=0.83,95%CI 0.74-0.94), p=0.002]. after birth began taking probiotics, there was no significant difference between the two groups (p=0.89). Conclusion: late pregnancy to early postnatal probiotics can reduce children. The incidence of dermatitis, and this effect can be maintained in the future five years. Objective: to screen the mouse hair cycle, the differentially expressed proteins in the skin. Methods: to establish the model of hair cycle in mice, different hair cycle (0d, 8D, 20 d) of the skin samples, protein extraction, were two dimensional gel electrophoresis and by mass spectrometry analysis, identification of differentially expressed proteins. Using bioinformatics, Gene Ontology carries on the preliminary analysis on its function. Results: there were 44 differential proteins by two-dimensional electrophoresis Mass spectrometry analysis and identification, selected growth expression of 3 proteins were up-regulated, Western blotting is verified. The biological information expression differences were found by protein and cell apoptosis, cell proliferation and differentiation and signal transduction and so on. Conclusion: the preliminary screening of different hair cycle in mice, 44 differentially expressed in the skin the presence of protein, can provide the direction for future research.

【学位授予单位】：南京医科大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R758.2

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