应用MALDI-TOF-MS技术分析转移和非转移黑色素瘤患者外周血清蛋白的差异表达

发布时间：2018-01-12 16:21

本文关键词：应用MALDI-TOF-MS技术分析转移和非转移黑色素瘤患者外周血清蛋白的差异表达　出处：《重庆医科大学学报》2013年05期 　论文类型：期刊论文

【摘要】：目的:应用蛋白质组学技术分析转移性和非转移性黑色素瘤患者外周血清中差异表达的蛋白质,初步探讨其对转移性黑色素瘤患者的诊断及治疗指导意义。方法:共收集101例患者外周血清,包括25例转移性黑色素瘤患者及76例对照者(31例非转移性患者和45例健康者)。从中随机抽取73例(18例转移患者,20例非转移患者和35例健康者)用于建立区分模型,其余28例(7例转移患者,11例非转移患者和10例健康者)用来对区分模型进行盲法验证。运用基质辅助激光解离飞行时间质谱技术,对外周血清蛋白质进行检测,应用Ciphergen蛋白质芯片软件对蛋白质谱进行分析。结果:黑色素瘤转移患者外周血清与对照组外周血清蛋白质谱相比,质荷比为2 598.33、3 646.79、4 605.48、8 603.10、7 756.64 m/z和9 598.82 m/z,共6个蛋白质峰呈表达有明显差异(P均=0.000),以此构建出转移性黑色素瘤的诊断模型,它的诊断敏感性为83.33%,特异性为74.55%。另外对该模型进行了盲法验证,其诊断转移性黑色素瘤的敏感性为71.43%,特异性为76.19%。结论:黑色素瘤转移患者外周血清蛋白质表达谱与对照组相比有明显变化,相对分子质量为2 598.33、3 646.79、4 605.48、8 603.10、7 756.64 m/z和9 598.82 m/z的蛋白质点可以作为黑色素瘤转移患者诊断的潜在生物学标志物。
[Abstract]:Objective: to analyze the differential expression of metastatic and non metastatic melanoma patients with peripheral serum protein by proteomics, explore the patients with metastatic melanoma diagnosis and treatment guidance. Methods: collected 101 cases of patients with serum, including 25 cases of patients with metastatic melanoma and 76 control subjects (31 cases of non metastatic patients and 45 healthy subjects). From randomly selected 73 cases (18 cases with metastasis, 20 cases of non metastatic patients and 35 healthy people) are used to establish the distinguishing model, the remaining 28 cases (7 cases with metastasis, 11 patients with non metastasis and 10 cases healthy) used for blind test to distinguish model. Using matrix assisted laser desorption ionization time-of-flight mass spectrometry, peripheral serum protein was detected on protein mass spectrometry analysis of the application of Ciphergen protein chip software. Results: in patients with metastatic melanoma in peripheral blood Compared with the control group, peripheral serum protein mass spectrometry, mass of 2 598.33,3 646.79,4 605.48,8 603.10,7 756.64 m/z and 9598.82 m/z, a total of 6 protein peaks showed expression had significant difference (P =0.000), in order to build a model for the diagnosis of metastatic melanoma, the diagnostic sensitivity was 83.33%, specificity was 74.55%. in addition to the model of the blind test, the sensitivity of diagnosis of metastatic melanoma was 71.43%, the specificity was 76.19%. conclusion: melanoma metastasis in patients with peripheral expression and the control group had significant changes compared to the serum protein of molecular mass as potential biomarkers for 2 598.33,3 646.79,4 605.48,8 756.64 m/z and 603.10,7 9598.82 points can be used as a m/z protein in patients with metastatic melanoma diagnosis.

【作者单位】：郑州大学第一附属医院整形外科;
【分类号】：R739.5
【正文快照】： 恶性黑色素瘤是一种容易发生转移、恶性程度极高的肿瘤,死亡率位居皮肤恶性肿瘤之首[1]。该病在我国的发病率有明显上升趋势,年增长率约3%~5%[2]。目前,对黑色素瘤最有效的治疗手段是早期诊断并进行外科手术,然而肿瘤一旦发生转移对现在的治疗手段常不敏感,总体中位生存期仅为

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