IL-9抗体对小鼠银屑病样皮损及IL-17和CCR6的影响

发布时间：2018-01-15 12:25

本文关键词：IL-9抗体对小鼠银屑病样皮损及IL-17和CCR6的影响　出处：《中国皮肤性病学杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨白介素(IL)-9抗体对小鼠银屑病样皮损及白介素(IL)-17与CC趋化因子受体(CCR)6的影响。方法 Balb/c雌性小鼠随机分为三组:凡士林空白对照组(空白对照组)、咪喹莫特模型组(模型组)以及IL-9抗体实验组(实验组)。通过PASI评分观察银屑病小鼠皮损严重程度变化,免疫组化法检测皮损中IL-17,CCR6的变化,Western blot检测皮损IL-17的表达。结果免疫组化结果表明,与空白对照组相比,模型组小鼠皮损中IL-17,CCR6的含量显著增高(P0.05);与模型组相比,实验组小鼠的皮损严重程度明显降低,炎症细胞浸润减轻,IL-17,CCR6表达亦明显降低(P0.05)。而Western blot也显示实验组与模型组皮损IL-17的差异有统计学意义(P0.05)。结论 IL-9抗体可能通过抑制CCR6,减少皮损中Th17细胞及树突状细胞的数目,以及抑制IL-17的含量,从而达到改善银屑病样皮损的目的。
[Abstract]:Objective to investigate the effects of IL-9 antibody on psoriasis-like skin lesions of mice and the relationship between IL-17 and CC chemokine receptor (CCR). Methods Balb/c female mice were randomly divided into three groups: Vaseline blank control group (blank control group). Imiquimod model group (model group) and IL-9 antibody experimental group (experimental group). The severity of psoriasis was observed by PASI score and IL-17 in psoriasis was detected by immunohistochemical method. The expression of IL-17 in skin lesions was detected by Western blot. Results the results of immunohistochemistry showed that compared with the blank control group, the IL-17 in the skin lesions of the model group was higher than that of the control group. The content of CCR6 increased significantly (P 0.05). Compared with the model group, the severity of skin lesions and the infiltration of inflammatory cells in the experimental group were significantly lower than those in the model group. The expression of CCR6 also decreased significantly (P 0.05), and Western blot also showed that there was a significant difference in IL-17 expression between the experimental group and the model group (P 0.05). Conclusion IL-9 antibody may inhibit CCR6. To reduce the number of Th17 cells and dendritic cells in lesions and to inhibit the content of IL-17, so as to improve psoriasis lesions.
【作者单位】：南方医科大学附属广东省第二人民医院皮肤科;南方医科大学第三临床医学院;
【基金】：广东省中医药局科研课题(NO.20141008)
【分类号】：R758.63
【正文快照】： 银屑病是一种常见的慢性炎症性疾病,以鳞屑性红斑为特征,被认为是一种Th17/Th1相关疾病,其中又以Th17及其相关细胞因子IL-17,IL-22等占主导地位[1]。研究报道银屑病患者及动物模型中血清及皮Blank control group;Model group;Experimental group图1背部皮损形态学观察Fig.1 Gr

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