Glut-1、VEGF、Ki-67在婴儿型血管瘤与皮下血管畸形表达及意义

发布时间：2018-01-27 16:10

本文关键词： 血管瘤血管畸形 Glut-1 VEGF Ki-67　出处：《新疆医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：检测葡萄糖转运蛋白-1（glucose transporter-1，Glut-1）、血管内皮生长因子（Vascular Endothelium Growth Factor, VEGF）、Ki-67在增殖期婴儿型血管瘤和血管畸形中的表达并探讨其意义。方法：选取新疆医科大学附属肿瘤医院2006年3月至2011年12月手术切除并经病理证实的增殖期婴儿型血管瘤和皮下血管畸形蜡块标本，常规石蜡切片苏木精-伊红染色法（hematoxylin-eosin staining，HE）染色，进行病理复检分组，其中增殖期血管瘤22例，年龄1月-12月，中位年龄6个月。皮下血管畸形50例，年龄10-41岁，中位年龄27岁。另取25例病理证实的皮肤雀斑样痣扩切标本周围正常血管做阴性对照，年龄12-37岁，中位年龄24岁。所有标本做厚度4μm连续切片，，常规脱蜡及水化，采用免疫组织化学（Immunohistochemistry，IHC）链霉菌抗生物素蛋白-过氧化物酶连结法（streptavidin-perosidase，SP）处理，高温高压修复抗原，每张切片于400倍镜下，挑取10个视野，每个视野计数100个血管内皮细胞，1000个细胞中含阳性细胞数，换算成阳性百分数，即阳性表达率，表达强度用+表示，+越多强度越强。淡黄色为弱阳性+，棕黄色为中阳性++，棕褐色为强阳性+++。采用独立样本X2检验对数据进行分析。结果：①Glut-1免疫组织化学：增殖期血管瘤组中的阳性率95.45%，皮下血管畸形及正常血管没有Glut-1表达。②VEGF免疫组织化学：增殖期血管瘤组中的阳性率86.36%，皮下血管畸形28.00%，正常血管24.00%。VEGF在增殖期血管瘤组中的表达率明显高于皮下血管畸形及正常血管，差异有极显著性（P0.01），皮下血管畸形与正常血管表达没有差别（P0.05）。③Ki-67免疫组织化学：增殖期血管瘤阳性率90.91%；皮下血管畸形组和正常血管组各有一例出现Ki-67阳性表达，阳性率分别为2.00%和4.00%。Ki-67在增殖期血管瘤组中的表达率明显高于皮下血管畸形及正常血管，差异有极显著性（P0.01），皮下血管畸形及正常血管表达没有差别（P0.05）。结论：Glut-1、VEGF和Ki-67的异常表达与血管瘤的发生、发展有一定的关系，Glut-1、VEGF和Ki-67可以作为血管瘤与血管畸形鉴别诊断的免疫标志物，特别是Glut-1与Ki-67可能具有更高的灵敏度和特异度。
[Abstract]:Objective: to detect glucose transporter-1 (Glut-1). Vascular Endothelium Growth Factor (VEGF). Expression and significance of Ki-67 in proliferative infantile hemangioma and vascular malformation. Methods:. Wax samples of proliferative infantile hemangioma and subcutaneous vascular malformation were selected from tumor Hospital affiliated to Xinjiang Medical University from March 2006 to December 2011. Hematoxylin-eosin stenting was stained by hematoxylin-eosin staining in paraffin sections. 22 cases of proliferative hemangioma were divided into two groups. The median age was 6 months from January to December. 50 cases of subcutaneous vascular malformation aged 10-41 years. The median age was 27 years. Another 25 skin freckle nevus specimens, aged 12-37 years, were taken as negative control. The median age was 24 years old. All the specimens were made 4 渭 m thick serial sections, conventional dewaxing and hydration, immunohistochemistry was used. Streptavidin-perosidase SP) was treated with Streptavidin-perosidase (SPN), and the antigen was repaired by high temperature and high pressure. Each section was selected under 400 times microscope, 10 visual fields were selected, 100 vascular endothelial cells were counted in each field, and 1 000 cells contained positive cells, which was converted into positive percentage, that is, positive expression rate. The more the intensity, the stronger the intensity. The light yellow is weak positive, the brown yellow is medium positive. Brown was strongly positive. The data were analyzed by X2 test of independent sample. Results: the positive rate was 95.45% in proliferative hemangioma group. The positive rate of Glut-1 in proliferative hemangioma group was 86.36 and that in subcutaneous vascular malformation was 28.00%. The expression rate of VEGF in proliferative hemangioma group was significantly higher than that in subcutaneous vascular malformation and normal vessels (P 0.01). There was no difference between subcutaneous vascular malformation and normal vascular expression. P0.05 and 3Ki-67 immunohistochemical staining showed that the positive rate of proliferative hemangioma was 90.91%. Positive expression of Ki-67 was found in one case in subcutaneous vascular malformation group and one case in normal vascular group. The positive rates of Ki-67 in proliferative hemangioma group were significantly higher than those in subcutaneous vascular malformation and normal vessels (P 0.01). There was no difference between subcutaneous vascular malformation and normal vascular expression (P 0.05). Conclusion the abnormal expression of Ki-67 and Ki-67 may be related to the occurrence and development of hemangioma. Glut-1VEGF and Ki-67 can be used as immune markers for differential diagnosis of hemangioma and vascular malformation, especially Glut-1 and Ki-67 may have higher sensitivity and specificity.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5

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