散血草对血管瘤裸鼠模型瘤体生长的影响及其机制探讨

发布时间：2018-02-01 07:18

本文关键词： 散血草激素血管瘤裸鼠　出处：《泸州医学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的：通过建立血管瘤裸鼠模型，观察散血草对血管瘤裸鼠模型生长的影响并探讨其可能的作用机制。方法：采用组织块移植法，在无菌条件下取婴幼儿增生期毛细血管瘤手术标本切成小块，植入裸鼠皮下，制成婴幼儿血管瘤裸鼠异种移植动物模型。移植60天后，取移植成功的实验动物模型建立随机对照实验，分为4个实验组，分别给予以下不同干预措施：散血草组在血管瘤瘤体表面外用自制的散血草搽剂每日一次；激素组在血管瘤瘤体内均匀注射曲安奈德注射液0.05ml(4mg／ml)一次；散血草加激素组瘤体表面外用散血草搽剂，并在瘤体内注射曲安奈德注射液0.05ml(4mg／ml)一次；对照组瘤体表面外用对照物每日一次。给予不同干预措施后，，通过对血管瘤裸鼠模型瘤体体积的测量，监测血管瘤在裸鼠模型体内的生长情况；用药后15天处死实验裸鼠模型，取出血管瘤组织制成切片，HE染色进行组织学分析，并作免疫组织化学（S-P）染色检测HIF-1α、bcl-2和Ki-67表达的阳性指数，分析血管瘤在裸鼠模型体内的增殖和凋亡情况。使用SPSS14.0统计软件对所有实验结果进行统计学分析，以P0.05为有显著性差异。结果：制作的血管瘤裸鼠模型生长良好，血管瘤瘤体体积大小均匀，经对照物、散血草、散血草加激素、激素处理15天后，对照组裸鼠模型血管瘤瘤体体积继续增大，标本大体及镜下观测血管瘤瘤体呈增生期改变；散血草组、激素组、散血草加激素组裸鼠模型血管瘤瘤体体积与对照组相比均有缩小，各组之间血管瘤瘤体体积也均有显著性差异，组织标本观察到血管减少，瘤体萎缩、退化，血管腔闭塞、破裂，血管间纤维脂肪组织浸润，其中散血草加激素组最明显，激素组次之，散血草组血管瘤瘤体缩小程度最小。散血草组、激素组、散血草加激素组HIF-1α、bcl-2和Ki-67阳性指数与对照组相比均有减少，而且各组间相比也均有显著性差异，HIF-1α、bcl-2和Ki-67阳性指数在散血草加激素组减少最明显，激素组次之，散血草组瘤体减少程度最小。结论：散血草外用可以抑制血管瘤裸鼠移植模型瘤体的增殖；散血草外用与糖皮质激素(曲安奈德)联合应用，对血管瘤裸鼠移植模型瘤体增殖的抑制作用强于单用散血草或糖皮质激素；单用散血草对血管瘤裸鼠移植模型瘤体增殖的抑制作用不及单用糖皮质激素；散血草外用对血管瘤裸鼠移植模型瘤体增殖的抑制作用，可能与下调HIF-1α、bcl-2和Ki-67的表达水平有关。
[Abstract]:Objective: to establish a nude mouse model of hemangioma, to observe the effect of Sanguisegrass on the growth of nude mice with hemangioma and to explore its possible mechanism. Methods: tissue mass transplantation was used. In the aseptic condition, the capillary hemangioma in the proliferative phase of infant was cut into small pieces and implanted into the subcutaneous of nude mice to make the xenotransplantation animal model of infantile hemangioma. After 60 days of transplantation, the xenotransplantation model was made. The successful experimental animal model was used to establish a randomized controlled experiment, which was divided into four experimental groups, and were given the following different intervention measures: the hemangioma group was treated with self-made Sanguisegrass liniment once a day on the surface of the hemangioma; In the hormone group, triamcinolone acetonide injection (0.05ml / ml) was injected evenly into the hemangioma. In the control group, the tumor surface was treated with Xanthoxylum liniment and triamcinolone acetonide injection (0.05 mg / ml) was injected into the tumor body. After different intervention measures were given, the growth of hemangioma in nude mice was monitored by measuring the volume of tumor in nude mice. 15 days after administration, the nude mice were killed, the hemangioma tissue was taken out and stained with HE for histological analysis, and HIF-1 伪 was detected by immunohistochemical staining. The positive index of bcl-2 and Ki-67 expression was used to analyze the proliferation and apoptosis of hemangioma in nude mice model. SPSS14.0 statistical software was used to analyze all the experimental results. Results: the model of hemangioma in nude mice grew well and the volume of hemangioma tumor was uniform. In the control group, the tumor volume of hemangioma in nude mice model continued to increase, and the changes of hemangioma tumor were observed under microscope and gross. Compared with the control group, the tumor volume of the hemangioma model of the control group was reduced, and the volume of the hemangioma tumor was significantly different among the three groups. The vasculization was observed in the tissue specimen. Tumor atrophy, degeneration, lumen occlusion, rupture, intervascular fibrous adipose tissue infiltration, in which the most obvious group, hormone group, hormone group, the smallest degree of reduction of hemangioma tumor. The positive index of HIF-1 伪 bcl-2 and Ki-67 in the hormone group and SXC plus hormone group were all decreased compared with the control group, and there was a significant difference in HIF-1 伪 between the two groups. The positive index of bcl-2 and Ki-67 decreased most obviously in the group of Sanguisuegrass plus hormone, followed by the group of hormone. Conclusion: the external application of Sanguisegrass can inhibit the proliferation of tumor in nude mice model of hemangioma transplantation. In combination with triamcinolone acetonide (triamcinolone acetonide), the inhibition of tumor proliferation in nude mice with hemangioma transplantation was stronger than that of single hemangioma grafts or glucocorticoids. The inhibitory effect of SXC on tumor proliferation in nude mice was not as good as that of glucocorticoid alone. The inhibitory effect of Sanguisegrass on the proliferation of hemangioma xenografts in nude mice may be related to the down-regulation of the expression of HIF-1 伪 bcl-2 and Ki-67.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285;R739.5

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