老化皮肤光学特征提取及其治疗过程监测

发布时间：2018-02-22 05:01

本文关键词： 皮肤老化光学模型多光子显微术光学相干层析成像技术纹理特征光学特征光治疗　出处：《福建师范大学》2011年博士论文　论文类型：学位论文

【摘要】：皮肤的老化分为两个过程,即：随年龄增加的自然老化和受外界紫外光照射引起的光老化。这两个老化过程总是相互交叉,相互影响的。胶原的变化是皮肤老化过程中最主要的表征量。本论文结合多光子显微技术、光学相干层析成像技术等先进的医学成像技术,从组织光学、形态学、光谱及纹理特征等方面研究活体皮肤自然老化和光老化的过程,并将结果用于评价皮肤的光治疗过程。具体的研究创新成果如下： 1.利用多光子显微技术和光学相干层析成像技术活体跟踪研究自然老化及光老化过程中皮肤胶原形态及光谱方面的变化。结果显示真皮的胶原尺寸不随着年龄的变化而变化,而胶原二次谐波强度及最大可探测深度随着年龄的变化而其较大变化。 2.把胶原纹理特征参数作为评价各种老化类型皮肤的新指标。具体的有胶原方向指数、胶原束间距离、胶原相关性、胶原熵、分形维数及光学特性参数等。结果显示：年轻皮肤的胶原方向指数小,趋向各向同性,而且胶原束间距小,纹理紧密,细纹多。而自然老化皮肤的胶原方向指数大,胶原间距大；光老化皮肤的胶原在纹理上没有特别的方向性,但是胶原间距很大。 3.把光学特性参数作为评价皮肤老化过程的新指标,通过光学相干层析成像技术获得小鼠皮肤不同层的衰减系数和皮肤表皮厚度随着年龄和紫外光辐照程度变化的曲线图：皮肤真皮层的衰减系数随着年龄的增大而减小,也受紫外光的影响。 4.结合多光子显微技术与光学相干层析成像技术等医学成像技术从形态学、纹理特征、组织光学的角度探讨各种光源辐照皮肤后,皮肤真皮胶原及表皮的反应,并跟踪随后的修复过程。从亚微米数量级到毫米数量级来获得光对各种类型皮肤的光治疗情况。提出治疗的关键点,并评价各种光源对不同类型皮肤治疗的效果：消融性的IPL, Nd:YAG只能用于改善细小的皱纹,而对于严重的光老化皮肤则没有明显的效果。消融性的Er:YAG, C02激光对皮肤作用强,会引起较严重的并发症,但是可以较好的应用于光老化皮肤的治疗。其结果对光美容临床应用具有较大的指导意义。
[Abstract]:The aging of the skin is divided into two processes: natural aging with age and photoaging caused by external ultraviolet light. The two aging processes always intersect each other. The changes of collagen are the most important characterizations of skin aging. In this paper, advanced medical imaging techniques, such as multiphoton microscopy, optical coherence tomography, etc., have been developed from tissue optics, morphology, histopathology, histopathology, histopathology, histopathology, and other advanced medical imaging techniques. The natural aging and photoaging of living skin were studied in terms of spectral and texture characteristics, and the results were used to evaluate the process of skin phototherapy. Specific research and innovation results are as follows:. 1. The changes of collagen morphology and spectrum during natural aging and photoaging were studied in vivo by using multiphoton microscopy and optical coherence tomography. The results showed that the collagen size of dermis did not change with age. The second harmonic intensity and maximum detectable depth of collagen vary greatly with age. 2. The characteristic parameters of collagen texture were taken as a new index to evaluate various aging skin types, including collagen orientation index, distance between collagen bundles, collagen correlation, collagen entropy, collagen orientation index, collagen correlation, collagen entropy, collagen correlation and collagen entropy. The results show that the collagen direction index of young skin is small, tends to be isotropic, and the distance between collagen bundles is small, the texture is tight, the fine grain is many, but the collagen direction index of natural aging skin is large. Collagen spacing is large; the collagen of photoaged skin is not particularly directional in texture, but the collagen spacing is very large. 3. Using the optical characteristic parameters as a new index to evaluate the aging process of skin, The attenuation coefficient of different layers of mouse skin and the curve of skin skin thickness with age and ultraviolet irradiation degree were obtained by optical coherence tomography. The attenuation coefficient of dermis decreased with age. Also affected by ultraviolet light. 4. Combining medical imaging techniques such as multi-photon microscopy and optical coherence tomography, the reaction of dermal collagen and epidermis to skin irradiated by various light sources was discussed from the point of view of morphology, texture and tissue optics. And follow up the subsequent repair process. From submicron to millimeter order of magnitude to get light for various types of skin phototherapy. Put forward the key points of treatment, The effects of various light sources on the treatment of different skin types were evaluated. The ablative IPL, Nd:YAG can only be used to improve the fine wrinkles, but not for the severe photoaged skin. The ablative er: YG, C02 laser has a strong effect on the skin. It can cause serious complications, but it can be applied to the treatment of photoaged skin. The results are of great guiding significance for the clinical application of photo-cosmetology.
【学位授予单位】：福建师范大学
【学位级别】：博士
【学位授予年份】：2011
【分类号】：R758.1

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