特应性皮炎小鼠模型VDRAs对TLR2,TLR4及CRAMP的调控作用

发布时间：2018-02-22 09:31

本文关键词： 特应性皮炎维生素受体激动剂 Toll受体 Toll受体鼠阳离子相关抗菌肽　出处：《中国皮肤性病学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的观察在特应性皮炎(AD)小鼠模型中维生素D受体激动剂(VDRAs)的干预对皮损以及Toll受体2,4(TLR2,TLR4)和鼠阳离子相关抗菌肽(CRAMP)的影响,并探讨其相互关联机制。方法将25只雌性BALB/c小鼠随机分为5组,分别为空白组、AD模型组、AD模型不干预组、PBS对照组、VDRAs治疗组,每组各5只。除空白组以外的其余4组,以二硝基氯苯(DNCB)诱导小鼠特应性皮炎模型。造模成功后,模型组直接处死,AD模型不干预组造模结束后不予以任何处理,VDRAs治疗组腹腔注射VDRAs,PBS对照组腹腔注射PBS液,两组均为每3日1次,总计5次。肉眼观察小鼠背部皮损变化,组织病理观察皮损厚度及炎细胞浸润情况。免疫组化观察各组皮损处TLR2,TLR4及CRAMP的表达部位及水平。Real-Time PCR测各组皮损中TLR2,TLR4及CRAMP的RNA含量,定量分析其变化。结果 VDRAs治疗组小鼠背部皮损炎症反应较AD模型不干预组和PBS对照组明显改善,免疫组化和Real-Time PCR示CRAMP的表达高于AD模型不干预组和PBS对照组,TLR2,TLR4表达较PBS对照组和AD模型组低,差异有统计学意义(P0.01)。结论在AD小鼠模型中,VDRAs抑制TLR2,TLR4表达,上调抗菌肽CRAMP的表达。
[Abstract]:Objective to observe the effects of vitamin D receptor agonist (VDRASS) on skin lesions and the effects of Toll receptor 2TLR2 (TLR4) and mouse cationic cationic antimicrobial peptide (CRAMP) in mice model of atopic dermatitis. Methods 25 female BALB/c mice were randomly divided into 5 groups: control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), control group (n = 5), and control group (n = 5). The model of atopic dermatitis in mice was induced by dinitrochlorobenzene (DNCBB). After the establishment of the model, the model group was killed directly and the model group was not treated with any intraperitoneal injection of PBS solution in the control group. The changes of skin lesions on the back of mice were observed with naked eyes. The expression and level of TLR2TLR4 and CRAMP in the lesions were observed by immunohistochemistry. Real-time PCR was used to detect the RNA content of TLR2TLR4 and CRAMP in the lesions of each group. Results the inflammatory reaction of the mice in the VDRAs treatment group was significantly improved than that in the AD model group and the PBS control group. Immunohistochemistry and Real-Time PCR showed that the expression of CRAMP was higher than that of non-intervention group and PBS control group, and the expression of TLR2nTLR4 was lower than that of PBS control group and AD model group, the difference was significant (P 0.01). Conclusion VDRAs can inhibit the expression of TLR2mTLR4 and up-regulate the expression of antimicrobial peptide CRAMP in AD mice.
【作者单位】：天津医科大学总医院皮肤性病科;
【基金】：国家自然科学基金青年科学基金项目(81301469)
【分类号】：R758.2;R-332

【相似文献】