肝X受体β在皮肤鳞状细胞癌中的表达及其临床意义

发布时间：2018-02-24 07:15

本文关键词： 肝X受体β 鳞状细胞癌日光性角化病鲍温病免疫组织化学染色　出处：《第三军医大学学报》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨肝X受体β(liver X receptorβ,LXRβ)在皮肤鳞状细胞癌(cutaneous squamous cell carcinoma,cSCC)中的表达及其意义。方法采用PCR array、Western blot检测LXRs在角质形成细胞(keratinocyte,KC)和皮肤鳞状细胞癌A431细胞系中的表达,并采用免疫组化Envision法检测LXRβ在52例日光性角化病(actinic keratosis,AK)、40例鲍温病(Bowen’s disease,BD)、60例cSCC中的表达,分析LXRβ表达与患者临床参数之间的关系。结果与KC比较,A431细胞中LXRα、LXRβ的mRNA和蛋白水平均升高,以LXRβ升高尤为显著。免疫组化染色结果发现LXRβ在AK、BD、cSCC中表达逐渐升高,以cSCC升高最明显,其表达与肿瘤分化程度、瘤体大小、浸润深度相关(P0.05),与年龄、性别、发病部位无关(P0.05)。结论 LXRβ在鳞状细胞癌细胞和组织中表达均升高,且与肿瘤的增殖、分化相关。
[Abstract]:Objective to investigate the expression and significance of liver X receptor 尾 receptor 尾 receptor 尾 (LXR 尾) in cutaneous squamous cell carcinoma (SCC). Methods the expression of LXRs in keratinocyte keratinocyte KC1 and squamous cell carcinoma A431 cell line was detected by PCR blot. Immunohistochemical Envision method was used to detect the expression of LXR 尾 in 60 cases of cSCC of 40 cases of Bowenia disease, and to analyze the relationship between the expression of LXR 尾 and the clinical parameters. Results compared with KC, the mRNA and protein levels of LXR 伪 LXR 尾 in KC cells were higher than those in KC cells. The expression of LXR 尾 was increased gradually, especially in cSCC. The expression of LXR 尾 was correlated with tumor differentiation, tumor size, depth of invasion, age and sex. Conclusion the expression of LXR 尾 in squamous cell carcinoma cells and tissues is increased, which is related to the proliferation and differentiation of the tumor.
【作者单位】：第三军医大学新桥医院皮肤科;第三军医大学西南医院全军临床病理学研究所;
【基金】：国家自然科学基金青年科学基金(81502369)~~
【分类号】：R739.5

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