女性无症状淋病患者胸腺基质淋巴细胞生成素的表达及与IL-17,IL-10关系

发布时间：2018-02-27 21:30

本文关键词： 淋球菌无症状感染孕激素胸腺基质淋巴细胞生成素　出处：《中国皮肤性病学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨女性无症状淋病患者中胸腺基质淋巴细胞生成素(TSLP)的表达及其与IL-17,IL-10水平的关系。方法选择123例女性无明显症状淋病患者为无明显症状组,32例有症状女性淋病患者为有症状组,放射免疫法(RIA)测定血清孕酮浓度;荧光定量PCR和酶联免疫吸附法(ELISA)在mRNA水平和蛋白水平检测宫颈分泌物中TSLP,IL-17和IL-10的水平。结果女性无明显症状淋病患者中TSLP表达升高,其表达与孕激素水平及IL-10呈显著正相关(r=0.99,0.97,P均0.01),与IL-17呈显著负相关(r=-0.89,P0.01)。有症状组和正常对照组TSLP和IL-10水平差异无统计学意义(P均0.05)。有症状组IL-17水平明显高于正常对照组,差异有统计学意义(P0.01)。结论孕激素可能通过上调TSLP表达在女性无症状淋球菌感染中发挥作用。
[Abstract]:Objective to investigate the expression of thymic stromal lymphopoietin (TSLP) in asymptomatic gonorrhea and its relationship with IL-17 IL-10 level. Methods 123 female patients with asymptomatic gonorrhea were selected as asymptomatic group of 32 symptomatic women. Gonorrhea patients were symptomatic, Radioimmunoassay (RIA) was used to detect serum progesterone concentration, fluorescence quantitative PCR and enzyme linked immunosorbent assay (Elisa) were used to detect the levels of TSLP IL-17 and IL-10 in cervical secretion at the level of mRNA and protein. There was a significant positive correlation between the expression of progesterone and the level of progesterone and IL-10 (P < 0.01), but a negative correlation with IL-17 (P < 0.05). There was no significant difference in the levels of TSLP and IL-10 between the symptomatic group and the normal control group (P < 0.05). The IL-17 level in the symptomatic group was significantly higher than that in the normal control group. Conclusion progesterone may play a role in asymptomatic gonococcal infection by upregulating the expression of TSLP.
【作者单位】：武汉市第一医院皮肤科;
【基金】：国家自然科学基金项目(81101201) 武汉市卫生计生委科研项目(WX15B13)
【分类号】：R759.2

【相似文献】