纳米雄黄对人皮肤鳞状细胞癌A431细胞株增殖抑制及诱导凋亡作用的研究

发布时间：2018-03-04 23:35

本文选题：纳米雄黄　切入点：人皮肤鳞状细胞癌A细胞株　出处：《中国实验方剂学杂志》2013年04期 　论文类型：期刊论文

【摘要】：目的:探讨纳米雄黄对人皮肤鳞癌A431细胞株增殖抑制及诱导凋亡作用及作用机制。方法:应用MTT比色法、流式细胞仪观察纳米雄黄体对人皮肤鳞状细胞癌A431细胞的增殖及凋亡情况、RT-PCR检测纳米雄黄对生存素(Survivin)mRNA,Caspase-3 mRNA表达的影响。结果:MTT实验及流式细胞仪提示纳米雄黄具有诱导皮肤鳞癌A431细胞凋亡和抑制细胞增殖作用,且随着纳米雄黄剂量的增加作用增强,与顺铂联合应用有协同作用;对照组,5%,10%,20%纳米雄黄组,DDP组,DDP+10%纳米雄黄组的Survivin相对表达量依次为(68.74±1.96)%,(63.93±3.57)%,(57.12±3.93)%,(47.64±6.44)%,(41.44±4.83)%及(32.18±4.10)%;Casepase-3相对表达量依次为(26.26±2.07)%,(32.660±4.42)%,(35.29±5.26)%,(38.52±8.37)%,(45.69±4.19)%及(52.98±6.52)%;纳米雄黄剂量增加可促进Caspase-3的表达,降低Survivin的表达。结论:纳米雄黄可通过诱导肿瘤细胞凋亡和抑制其增殖发挥抗肿瘤作用;其作用机制可能与上调Caspase-3的表达和下调Survivin的表达有关。
[Abstract]:Objective: to investigate the effect and mechanism of nanometer realgar on proliferation inhibition and apoptosis induction of human cutaneous squamous cell carcinoma cell line A431. Methods: MTT colorimetric assay was used. Flow cytometry was used to observe the proliferation and apoptosis of human skin squamous cell carcinoma A431 cells induced by nano-realgar body. RT-PCR was used to detect the effect of nano-realgar on the expression of Caspase-3 mRNA in survivin survivin mRNAs. Inducing apoptosis and inhibiting cell proliferation of skin squamous cell carcinoma A431 cells. With the increase of the dosage of nano-realgar, the synergistic effect with cisplatin was observed. The relative expression of Survivin was 68.74 卤1.96 卤63.93 卤3.577.12 卤3.973 卤3.973 卤57.64 卤6.4443% in the control group, 41.44 卤4.83% in the control group and 32.18 卤4.10% in the control group. The relative expression of Caspase-3 was 26.26 卤2.07 and 32.26 卤2.07 respectively in the DDP 10% group, and 38.52 卤8.37 卤4.37 卤45.69 卤4.19% and 52.98 卤6.52%, respectively; the increase of realgar yellow could promote the expression of Caspase-3. Conclusion: Realgar can induce apoptosis and inhibit the proliferation of tumor cells, and its mechanism may be related to up-regulation of Caspase-3 expression and down-regulation of Survivin expression.
【作者单位】：山东中医药大学附属医院;山东中医药大学;
【基金】：山东省自然科学基金项目(ZR2010HL050)
【分类号】：R285.5

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