外周血Treg细胞及相关因子水平与CSU发病的相关性

发布时间：2018-03-11 18:39

本文选题：慢性自发性荨麻疹　切入点：Treg细胞　出处：《兰州大学》2017年硕士论文　论文类型：学位论文

【摘要】：背景慢性自发性荨麻疹(chronic spontaneous urticaria,CSU),又称慢性荨麻疹,是指因为真皮内小血管扩张、改变了皮肤的渗透性而引起的皮肤水肿,特点为病程长于6周,且出现风团伴或不伴血管性水肿者。由于慢性自发性荨麻疹病程时间长,病因不明,常规抗组胺治疗容易复发,影响患者的正常生活。是以CSU发病机制的研究一直是皮肤科的热点。近年来,Th1/Th2细胞失衡及趋化因子的异常表达已被认为是慢性自发性荨麻疹发病机制的重要因素,但其调节机制未有报道。调节性T细胞(Treg cells,Treg细胞)及其相关细胞因子在变态反应性疾病中起重要的调节作用,但其在慢性自发性荨麻疹中的调节作用研究较少。目的本研究通过检测慢性自发性荨麻疹人群及健康人群外周血Treg细胞比例及白细胞介素-17(Interleukin-17,IL-17),白细胞介素-27(Interleukin-27,IL-27),白细胞介素-35(Interleukin-35,IL-35),转化生长因子-β1(transforming growth factor-β1,TGF-β1)的水平变化,探讨Treg细胞及相关细胞因子在CSU疾病发生中的作用。方法1.收集2016年1月—2016年6月兰州大学第一医院皮肤科的37例慢性自发性荨麻疹患者(男19人,女18人,年龄40.41±15.33岁)和健康体检者40例(男21人,女19人,年龄41.85±14.44岁)的外周血,进行流式细胞术检测采集的血液中Treg细胞比例,酶联免疫法(ELISA)检测血液上清液中IL-17和IL-27的水平。2.收集2014年1月—2015年12月兰州大学第一医院皮肤科45例慢性自发性荨麻疹患者(男17例,女28例,18-73岁,年龄39.13±13.17岁)和健康体检者43例(男12例,女31例,年龄18-60岁,年龄29.44±9.89岁)的外周血,进行酶联免疫吸附试验(ELISA)检测血清中白介素-35及TGF-β1的水平。结果1.外周血Treg细胞百分比及白介素-17、白介素-27的表达CSU患者中的Treg百分比和血清白介素-27水平均比对照组低(p=0.0030.05);白介素-17表达两组之间无统计差异;Treg细胞百分比例与白介素-17水平表达呈负性关系(r=-0.359,p0.05),与白介素-27间无明显关系;Treg细胞百分比、IL-17水平、IL-27水平与UAS分数均无统计相关关系。2.血清IL-35、TGF-β1水平表达CSU患者血清中的IL-35、TGF-β1水平均高于对照组(p0.05),且IL-35水平与TGF-β1水平间无明显相关性(p=0.589),Spearman分析显示二者与疾病时间长短均无明显相关关系(p0.05)。结论慢性自发性荨麻疹人群血清中白介素-35、TGF-β1水平比对照组高,表明Treg有可能通过产生的白介素-35、TGF-β1在慢性自发性荨麻疹的慢性损伤过程中起一定的作用。CSU人群外周血中白介素-27表达及Treg百分比较对照低,且白介素-17水平与Treg百分比呈负性关系,提示慢性自发性荨麻疹人群外周血中可能存在Th17/Treg的失衡,且IL-27可能与慢性自发性荨麻疹的发病过程相关。
[Abstract]:Background chronic spontaneous urticaria, also known as chronic urticaria, refers to skin edema caused by small intradermal vasodilation, which changes the permeability of the skin. Because of the long course of chronic spontaneous urticaria and the unknown etiology, routine antihistamine therapy was easy to relapse. In recent years, the imbalance of Th1 / Th2 cells and abnormal expression of chemokines have been considered as important factors in the pathogenesis of chronic spontaneous urticaria. However, its regulatory mechanism has not been reported. The regulatory T cell / Treg cell / Treg cell) and its related cytokines play an important role in the regulation of allergic diseases. But its regulatory role in chronic spontaneous urticaria is less studied. Objective to detect the proportion of Treg cells in peripheral blood of patients with chronic spontaneous urticaria and healthy people, and to detect the proportion of interleukin-17 (IL-17), interleukin-27 (IL-27), interleukin-27 (IL-27) and white blood cells (WBC) in patients with chronic spontaneous urticaria. Interleukin-35 (IL-35), transforming growth factor- 尾 1 (TGF- 尾 1) and transforming growth factor- 尾 1 (TGF- 尾 1), and transforming growth factor- 尾 1 (TGF- 尾 1), and transforming growth factor- 尾 1 (TGF- 尾 1). Methods 1. From January 2016 to June 2016, 37 patients with chronic spontaneous urticaria (male 19, female 18) in Department of Dermatology, first Hospital of Lanzhou University were collected. The percentage of Treg cells in blood was detected by flow cytometry in 40 patients (male 21, female 19, age 41.85 卤14.44 years) and 40 healthy subjects (aged 40.41 卤15.33 years), and 40 healthy subjects (male 21, female 19, age 41.85 卤14.44) were examined by flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect the levels of IL-17 and IL-27 in blood supernatant. From January 2014 to December 2015, 45 patients (17 males and 28 females aged 18 to 73 years) with chronic spontaneous urticaria in Department of Dermatology, first Hospital of Lanzhou University were collected. Age 39.13 卤13.17 years old and 43 healthy subjects (12 males, 31 females, aged 18-60 years, 29.44 卤9.89 years). Serum levels of interleukin-35 and TGF- 尾 _ 1 were determined by Elisa. Results 1.The percentage of Treg cells in peripheral blood and the expression of interleukin-17 and interleukin-27 in patients with CSU were higher than those in patients with CSU. There was no statistical difference between the expression of IL-17 and the expression of IL-17 in the control group. There was a negative relationship between the percentage of Treg cells and the expression of IL-17. There was no significant correlation between the percentage of Treg cells and the percentage of Treg cells, the level of IL-27 and the UAS score. There was no statistical correlation between serum IL-35 and TGF- 尾 1 levels in patients with CSU. The serum levels of IL-35 and TGF- 尾 1 in patients with CSU were significantly higher than those in the control group (p0.05), and there was no significant correlation between the levels of IL-35 and TGF- 尾 1. The results of Spearman analysis showed that there was no significant correlation between the levels of IL-35 and TGF- 尾 1 and the duration of the disease. Conclusion the serum levels of TGF- 尾 1 in patients with chronic spontaneous urticaria were higher than those in the control group. It is suggested that Treg may play a role in chronic injury of chronic urticaria by producing interleukin-35 TGF- 尾 1. The expression of interleukin-27 and the percentage of Treg in peripheral blood of Treg population are lower than those of control group. The level of interleukin-17 was negatively correlated with the percentage of Treg, suggesting that there may be imbalance of Th17/Treg in peripheral blood of patients with chronic spontaneous urticaria, and IL-27 may be related to the pathogenesis of chronic spontaneous urticaria.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R758.24

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