亚临床型生殖器疱疹患者脱排病毒与机体免疫状态的相关性研究

发布时间：2018-03-15 12:07

本文选题：亚临床型生殖器疱疹排毒　切入点：IL-2　出处：《桂林医学院》2010年硕士论文　论文类型：学位论文

【摘要】：目的探讨亚临床型生殖器疱疹患者脱排病毒与患者机体免疫状态的相关性。方法收集2009年1-11月我校附属医院皮肤性病科门诊亚临床型生殖器疱疹(Subclinical genital herpes,Subclinical GH)患者60名,用荧光定量聚合酶链式反应(Fluorescence quantitative Polymerase Chain Reaction ,FQ-PCR)测定病毒脱排情况,并用酶联免疫吸附剂试验(enzyme linked immunosorbent assay,ELISA)测定患者血清白介素2(interleukin-2,IL-2)、干扰素γ(interferon-γ,IFN-γ)水平和血清抗体。采用PEMS3.1统计软件进行相关分析。结果60例亚临床型生殖器疱疹患者标本中有23例单纯疱疹病毒核酸检测呈阳性反应,阳性率为38.33%。亚临床型生殖器疱疹患者组血清IL-2水平明显低于正常对照组,差异有显著性(t=27.0499,p0.05);患者组血清IFN-γ水平明显低于正常对照组,差异有显著性(t=10.2462,p0.05)。亚临床型生殖器疱疹患者脱排病毒阳性组血清IL-2水平明显低于阴性组,差异有显著性(t’=11.0866,p0.05);脱排病毒阳性组患者血清IFN-γ水平明显低于阴性组,差异有显著性(t’=10.1519,p0.05)。60例亚临床型生殖器疱疹患者血清抗体阳性率为71.67%,其中血清抗体阳性组脱排病毒阳性率为30.23%,明显低于血清抗体阴性组(58.82%),差异有显著性(χ2=4.2131,p=0.040.05);血清抗体阳性组血清IL-2水平明显高于血清抗体阴性组,差异有显著性(t =3.5741,p0.05);血清抗体阳性组血清IFN-γ水平明显高于血清抗体阴性组,差异有显著性(t’=5.1005,p0.05)。结论1、亚临床型生殖器疱疹患者尽管无明显临床症状,但存在无症状脱毒现象,是生殖器疱疹一个重要的传染源,应予以高度重视。2、亚临床型生殖器疱疹患者中,血清抗体阳性组脱排病毒阳性率明显低于血清抗体阴性组,并且血清抗体阳性组血清IL-2、IFN-γ明显高于血清抗体阴性组,说明生殖器疱疹血清抗体的产生对限制病毒的复制有一定的作用。3、亚临床型生殖器疱疹患者血清IL-2、IFN-γ水平低于正常人群血清水平,并且生殖器疱疹病毒脱排阳性组患者血清IL-2、IFN-γ水平低于疱疹病毒脱排阴性组患者,提示亚临床型生殖器疱疹患者细胞免疫功能低下,在一定条件下容易激发致病。
[Abstract]:Objective to investigate the relationship between the immune status of patients with subclinical genital herpes. Methods A total of 60 patients with subclinical genital herpes herpes were collected from 2009 to November in the Department of Dermatology and venereal Disease, affiliated Hospital of our University. The virus demotion was determined by fluorescence quantitative polymerase chain reaction (FQPCR) and fluorescence quantitative polymerase chain reaction (FQ-PCR). The serum levels of interleukin-2 interleukin-2 interleukin-2 and interferon- 纬 interferon- 纬 (IFN- 纬) and serum antibodies were determined by enzyme linked immunosorbent assaysa. The correlation was analyzed by PEMS3.1 software. Results among the 60 cases of subclinical genital herpes, 23 cases of herpes simplex virus nucleic acid showed positive reaction, the positive rate was 38.33. The serum IL-2 level of patients with subclinical genital herpes was significantly lower than that of normal control group. The serum level of IFN- 纬 in the patients group was significantly lower than that in the normal control group (P 0.05). The serum IL-2 level in the patients with subclinical genital herpes was significantly lower than that in the negative group, and the level of serum IFN- 纬 in the patients with subclinical herpes genitalis was significantly lower than that in the negative group. The level of IFN- 纬 in the patients with positive apoplexy virus was significantly lower than that in the negative group, there was a significant difference between the two groups, and the level of IFN- 纬 in the positive group was significantly lower than that in the negative group. The positive rate of serum antibody in patients with subclinical herpes genitalis was 71.677.The positive rate of serum antibody positive group was 30.23 and was significantly lower than that of serum-negative group (蠂 24.2131p0.040.05), and the positive rate of serum antibody positive was 30.23g, significantly lower than that of serum-negative group (蠂 24.2131p0.040.05). The serum IL-2 level in sex group was significantly higher than that in serum antibody negative group. The level of IFN- 纬 in serum antibody positive group was significantly higher than that in serum antibody negative group, and there was significant difference in serum IFN- 纬 level between serum antibody positive group and serum antibody negative group. Conclusion 1. Although subclinical genital herpes patients have no obvious clinical symptoms, but there is asymptomatic detoxification phenomenon, it is an important source of infection for genital herpes. The positive rate of serum antibody positive group was significantly lower than that of serum antibody negative group, and the serum IL-2 IFN- 纬 level in serum antibody positive group was significantly higher than that in serum antibody negative group. The results showed that the production of serum antibodies to genital herpes had a certain effect on limiting the replication of the virus, and the serum levels of IL-2mIFN- 纬 in patients with subclinical genital herpes were lower than those in normal subjects. The serum levels of IL-2mIFN- 纬 in patients with genital herpes virus (HSV) positive were lower than those in patients with HSV negative, suggesting that the cellular immune function of patients with subclinical herpes genitalis was low, and it was easy to cause disease under certain conditions.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R752.1

【参考文献】