转铁蛋白与RGD共修饰PLGA纳米粒的制备及其对黑色素瘤的靶向性研究

发布时间：2018-03-16 14:45

本文选题：转铁蛋白　切入点：RGD　出处：《中国生化药物杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的构建转铁蛋白(transferrin,TF)与RGD(精氨酸-甘氨酸-天冬氨酸,Arg-Gly-Asp)共修饰PLGA(聚乳酸羟基乙酸,poly(lactic-co-dycolic acid)纳米粒(TF/RGD-NPs),研究其黑色素瘤靶向性。方法采用乳化法制备TF和RGD共修饰纳米粒(TF/RGD-NPs),考察其形态、粒径、电位等理化性质。通过细胞摄取实验和黑色素瘤肿瘤球穿透实验考察TF/RGD-NPs与黑色素瘤B16细胞的亲和力和肿瘤组织穿透能力。结果制备的TF/RGD-NPs粒径为(113.4±12.5)nm,电位为(4.53±2.15)mV。体外细胞摄取实验表明B16细胞对TF/RGD-NPs的摄取效率分别是TF-NPs和RGD-NPs的2.7倍和2.9倍,差异均具有统计学意义(P0.01)。细胞摄取实验和肿瘤球摄取实验结果表明TF/RGD-NPs具有良好的黑色素瘤细胞亲和力。结论转铁蛋白与RGD共修饰纳米粒具有良好的黑色素瘤靶向性,是一种潜在的黑色素瘤靶向给药系统。
[Abstract]:Objective to construct TFRGD-NPsC (transferrin) and RGD (arginine-glycine-aspartic acid) co-modified PLGA (poly lactic-co-dycolic acid) nanoparticles, and to study the melanoma targeting of TFRGD-NPs.Methods TF and RGD co-modified TFGD-NPsn were prepared by emulsification method, and their morphology was investigated. Particle size, The affinity of TF/RGD-NPs to melanoma B16 cells and the penetrating ability of tumor tissue were investigated by cell uptake test and tumor ball penetration test. The results showed that the diameter of TF/RGD-NPs was 113.4 卤12.5 nm and the potential was 4.53 卤2.15 MV in vitro. The uptake efficiency of TF/RGD-NPs in B16 cells was 2.7 times and 2.9 times higher than that of TF-NPs and RGD-NPs, respectively. The results of cell uptake test and tumor ball uptake test showed that TF/RGD-NPs had good melanoma cell affinity. Conclusion Transferrin and RGD co-modified nanoparticles have good melanoma targeting ability. It is a potential melanoma targeting drug delivery system.
【作者单位】：华中农业大学医院;
【基金】：国家自然科学基金(81171365)
【分类号】：R739.5

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