犬小孢子菌丝氨酸水解酶FSH1全长cDNA的克隆及序列分析

发布时间：2018-03-17 01:37

本文选题：头癣　切入点：RACE　出处：《中国皮肤性病学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的克隆犬小孢子菌丝氨酸水解酶FSH1基因全长c DNA,为进一步研究FSH1的功能以及其在头癣中的致病机理打下基础。方法以犬小孢子菌的总RNA为材料,采用c DNA末端快速扩增(Rapid c DNA end amplification,RACE)方法扩增犬小孢子菌丝氨酸水解酶FSH1基因的全长c DNA。结合生物学软件对其序列进行了生物信息学分析。结果该全长c DNA大小为945bp,包含44bp的5’UTR、70bp的3’UTR、开放阅读框为831bp,编码276个氨基酸。Blast搜索结果显示,该全长c DNA与已报道的编码犬小孢子菌的结构域蛋白的基因序列同源性达100%,与编码石膏样小孢子菌的结构域蛋白基因序列同源性达78%。结论首次成功获得犬小孢子菌丝氨酸水解酶FSH1基因的全长c DNA,为进一步研究其在头癣发病中的功能奠定了基础。
[Abstract]:Objective to clone the full-length FSH1 gene of microsporum canis serine hydrolase (FSH1) gene in order to further study the function of FSH1 and its pathogenic mechanism in tinea capitis. Methods the total RNA of microsporozoa canis was used as the material. The full-length c DNAof the serine hydrolase FSH1 gene of microsporum canis was amplified by rapid c DNA end amplification race with c DNA terminal. The sequence was analyzed by bioinformatics with the combination of biological software. The results showed that the total length of c DNA was as follows:. 945bp, containing 44bp 5- UTR70bp, ORAF 831bp, encoding 276 amino acids. Blast search results show that, The sequence homology of the full-length c DNA with the reported domain protein of microsporozoa canis is 100, and that with the sequence of domain protein encoding microsporum gypsum is 78. Conclusion it is the first time to successfully obtain the gene sequence of domain protein of microsporum canis. The full-length cDNA of FSH1 gene of hyphae aminohydrolase lays a foundation for further study of its function in the pathogenesis of tinea capitis.
【作者单位】：大连市友谊医院皮肤性病科;大连医科大学附属第一医院皮肤性病科;
【基金】：国家自然科学基金(81071330,30640016)
【分类号】：R756.1

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