CXCL10、CXCR3及T细胞亚群在进展期白癜风患者外周血中的表达及复方中药对其影响

发布时间：2018-03-17 05:20

本文选题：白癜风　切入点：中草药　出处：《重庆医学》2018年02期 　论文类型：期刊论文

【摘要】：目的检测进展期白癜风患者外周血中T细胞亚群及趋化因子配体10(CXCL10)及其受体3(CXCR3)的表达情况,并探索复方中药对其影响。方法采用流式细胞仪检测治疗前后白癜风患者外周血中T细胞亚群的细胞比例,ELISA法检测治疗前后白癜风患者外周血清中CXCL10及CXCR3水平。结果服用复方中药1个月后,白癜风患者外周血中CD3~+CD4~+、CD3~+CD8~+T细胞比例均较治疗前升高(P0.05)。治疗前外周血清中CXCL10水平较健康对照组明显升高(P0.01),治疗后较治疗前明显降低(P0.05)。治疗前外周血清中CXCR3水平较健康对照组明显升高(P0.01),治疗后仍明显高于健康对照组(P0.01)。结论 CXCL10、CXCR3表达水平及T细胞亚群比例异常可能与白癜风的发病相关,本研究所用的复方中药可能通过调节T细胞亚群、CXCL10及CXCR3的表达水平发挥其治疗作用。
[Abstract]:Objective to detect the expression of T cell subsets and chemokine ligand 10 (CXCL10) and its receptor 3 (CXCR3) in peripheral blood of patients with vitiligo. Methods the ratio of T cell subsets in peripheral blood of patients with vitiligo before and after treatment was detected by flow cytometry and the levels of CXCL10 and CXCR3 in peripheral blood of patients with vitiligo before and after treatment were detected by Elisa. Results one month after taking compound Chinese medicine, The ratio of CD3 ~ CD4 ~ + CD3 ~ CD8 ~ T cells in peripheral blood of patients with vitiligo was higher than that before treatment. The level of CXCL10 in peripheral blood before treatment was significantly higher than that in healthy control group, and it was significantly lower after treatment than that before treatment. CXCR3 water in peripheral serum before treatment was significantly lower than that in control group, and the ratio of CD3 ~ CD4 ~ CD8 ~ T cells in peripheral blood of patients with vitiligo was significantly higher than that of patients with vitiligo before treatment. The level of CXCL10CXCR3 expression and the proportion of T cell subsets may be related to the pathogenesis of vitiligo. The compound Chinese medicine used in this study may play a therapeutic role by regulating the expression of CXCL10 and CXCR3 in T cell subsets.
【作者单位】：重庆市中医院/重庆市第一人民医院皮肤科;西南医科大学附属医院皮肤科;
【基金】：国家自然科学基金(81503588) 重庆市卫生和计划生育委员会基金项目(ZY20140251) 重庆市科技委员会基础与前沿研究计划(cstc2015jcyjA10113)
【分类号】：R758.41

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