尖锐湿疣皮损中树突状细胞分子表型的临床意义

发布时间：2018-03-18 20:30

本文选题：尖锐湿疣　切入点：人乳头瘤病毒　出处：《中国皮肤性病学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的检测尖锐湿疣(CA)皮损中树突状细胞CD1a,CD83,CD80和CD86的表达情况,并探讨其分子表型在CA发病中的作用及其与HPV亚型的相关性。方法采用HPV基因分型试剂盒检测CA患者所感染的HPV亚型,用免疫组化方法检测41例CA和32例正常包皮组织中CD1a,CD83,CD80和CD86的表达情况。结果 CA组织表皮CD1a阳性细胞数显著高于正常包皮组,且长病程组高于短病程组,高危型HPV感染组显著高于低危型HPV感染组,复发组略高于初发组,但差别无统计学意义。CA组织表皮内CD83阳性细胞数显著高于正常包皮组,CA组中,CD83表皮阳性细胞数与CD1a表皮阳性细胞数呈正相关,但较后者显著减少。CA组织表皮内CD80/CD86阳性细胞数与正常包皮组差异无统计学意义。结论 CA组织表皮中的树突状细胞大部分处于未成熟状态,缺乏共刺激分子(如CD80/CD86)的表达,尤其是伴有高危型HPV感染的病例,这可能是导致HPV抗原提呈障碍,从而造成CA复发及病程延长的免疫学机制之一。
[Abstract]:Objective to detect the expression of CD83T CD80 and CD86 in dendritic cells in the lesions of condyloma acuminatum. To explore the role of its molecular phenotype in the pathogenesis of CA and its correlation with HPV subtypes. Methods HPV genotyping kit was used to detect HPV subtypes infected by CA patients. The expression of CD83pCD80 and CD86 in 41 cases of CA and 32 cases of normal prepuce tissues were detected by immunohistochemistry. Results the number of CD1a positive cells in the epidermis of CA tissue was significantly higher than that in the normal prepuce group, and the number of CD1a positive cells in the long course group was higher than that in the short course group. The high risk HPV infection group was significantly higher than the low risk type HPV infection group, and the recurrence group was slightly higher than the initial group. The number of CD83 positive cells in the epidermis of CA group was significantly higher than that in the normal prepuce group. There was a positive correlation between the number of CD83 positive cells and the number of CD1a epidermal positive cells in CA group. However, there was no significant difference in the number of CD80/CD86 positive cells between CA and normal prepuce. Conclusion most of dendritic cells in CA epidermis are immature and lack expression of costimulatory molecules (such as CD80 / CD86). Especially in the patients with high risk HPV infection, this may be one of the immunological mechanisms that lead to HPV antigen presentation obstacle, thus lead to recurrence of CA and prolonged course of disease.
【作者单位】：民航总医院病理科;民航总医院皮肤科;
【基金】：民航总医院院级课题(编号2012020)
【分类号】：R752.53

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