慢性荨麻疹患者CR1基因多态性及红细胞表面免疫相关分子表达研究

发布时间：2018-03-18 23:33

本文选题：慢性荨麻疹　切入点：红细胞　出处：《右江民族医学院》2017年硕士论文　论文类型：学位论文

【摘要】：慢性荨麻疹的发病机制尚不清楚,免疫功能障碍是一个重要因素。红细胞免疫是人体天然免疫的重要组成部分,许多疾病的发病机制与红细胞免疫功能异常有关。现今国内外对于慢性荨麻疹红细胞免疫功能相关的研究报告较少,且不够深入,研究方法亦多采用传统的红细胞C3b受体(RBC-C3b)花环试验和红细胞免疫复合物(RBC-IC)花环试验,所获得的实验结果不尽相同,但大多数报告的观点是慢性荨麻疹患者存在红细胞免疫功能亢进和紊乱。现有研究表明,红细胞作为机体血循环含量最多的血细胞,表达多种天然免疫受体和物质,如CR1、CR3、CD44、CD58、CD59、DAF、SOD酶、趋化因子受体等。在机体天然免疫反应中及T淋巴细胞、B淋巴细胞特异免疫反应中,红细胞都占有重要地位,它参与了机体的免疫调控,对特异性免疫应答中关于抗原选择与应答类型有指导作用。近年来,对系统性红斑狼疮、恶性肿瘤、肝炎等疾病的红细胞免疫功能研究已取得了很大进展,并发现大多数免疫相关性疾病的红细胞免疫功能显著降低。慢性荨麻疹患者红细胞免疫功能状况究竟如何,红细胞表面免疫分子的表达是否存在变化以及其在慢性荨麻疹发病机制中的作用值得我们去深入研究。本课题通过从不同方面、不同水平分别对慢性荨麻疹患者红细胞免疫功能进行研究,包括红细胞表面CD35、CD44、CD55、CD58、CD59等分子表达情况,以及红细胞CR1密度相关基因的分布频率,以探讨红细胞免疫在慢性荨麻疹发病机制中的作用。对慢性荨麻疹红细胞免疫进行深入研究将有助于对该病的全面理解,开辟新的途径来了解该病的致病机制。目的:检测慢性荨麻疹患者红细胞表面天然免疫分子(CD35、CD44、CD55、CD58、CD59)表达情况,了解慢性荨麻疹患者红细胞免疫状况;以及慢性荨麻疹患者红细胞CR1密度相关基因多态性,探讨慢性荨麻疹患者红细胞CR1密度相关基因型的频率分布。方法:根据纳入标准分别设正常对照组和慢性荨麻疹组,抽取新鲜血液,用流式细胞仪定量检测红细胞表面CD35、CD44、CD55、CD58、CD59的平均荧光强度。提取研究对象DNA,采用聚合酶链式反应-限制性片段长度多态性分析(PCR-RFLP)方法测定红细胞CR1密度相关基因多态性。结果:(1)与对照组相比,慢性荨麻疹患者组红细胞CD35、CD55、CD58表达显著升高(P0.05),CD44、CD59表达显著降低(P0.05)。(2)慢性荨麻疹组中CR1基因HH、HL和LL基因型频率分别为74.2%、21.5%和4.3%,对照组HH、HL和LL基因型频率分别为71.8%、23.6%和4.6%,两组CR1基因型的分布频率差异无显著性(P0.05)。结论:(1)慢性荨麻疹患者红细胞CD35分子的数量较正常人增高,提示慢性荨麻疹患者的红细胞免疫功能存在亢进和紊乱。红细胞CD35分子数量增多可能是慢性荨麻疹天然免疫反应能力亢进和紊乱的基础。(2)慢性荨麻疹红细胞表面CD44表达降低,CD55、CD58、CD59表达升高,可能对慢性荨麻疹的发病有一定影响。(3)慢性荨麻疹患者红细胞CD35数量的改变与CR1密度相关基因的遗传关系不密切,可能是后天获得性因素影响所致。
[Abstract]:The pathogenesis of chronic urticaria is unclear, immune dysfunction is an important factor. Red cell immunity is an important component of human innate immunity in the pathogenesis of many diseases, and erythrocyte immune function abnormalities. At home and abroad for chronic urticaria of red cell immune function related research report less, and in-depth, research methods also the traditional red cell C3b receptor rosette test (RBC-C3b) and red blood cell immune complex rosette test (RBC-IC), the experimental results are not the same, but most of the reported point of view is that the red cell immune function in patients with chronic urticaria and hyperactivity disorder exists. The existing research shows that red blood cells as the blood cells of the body most of the content of blood circulation, the expression of a variety of innate immune receptors and substances, such as CR1, CR3, CD44, CD58, CD59, DAF, SOD enzyme, chemokine receptor of innate immunity in the body. Immune response and T lymphocyte, B lymphocyte specific immune response, red blood cell plays an important role, it is involved in the immune regulation, the specific immune response of antigen selection and response type guiding role. In recent years, the systemic lupus erythematosus, malignant tumor, immunologic function of red blood cell hepatitis other diseases have made a great progress, and found that most of the red cell immune function in immune related diseases were significantly decreased. Function of red blood cells in patients with chronic urticaria by how the expression of surface of red blood cell immune molecules, whether there are changes and its role in the pathogenesis of chronic urticaria is worth researching. This paper from different aspects of different levels were studied on red cell immune function in patients with chronic urticaria, including red blood cell surface CD35, CD44, CD55, CD58, CD59 etc. The molecular expression, and the density of CR1 in red blood cell related gene frequency distribution, to explore the role of red blood cell immunity in the pathogenesis of chronic urticaria. The chronic urticaria of red cell immune in-depth study will contribute to a comprehensive understanding of the disease, open up a new way to understand the pathogenesis of this disease. Objective: to detect the the surface of red blood cells in patients with chronic urticaria of innate immune molecules (CD35, CD44, CD55, CD58, CD59) expression, understanding of red cell immune in patients with chronic urticaria; and chronic urticaria patients erythrocyte CR1 density related gene polymorphism, the frequency distribution of chronic urticaria patients erythrocyte CR1 density associated genotype. Methods: according to the the inclusion criteria were divided into normal group and chronic urticaria group, extraction of fresh blood were detected by flow cytometry and red cell surface CD35, CD44, CD55, CD58, CD59 average fluorescence Study on the extraction of strength. The object DNA, using restriction fragment length polymorphism analysis of polymerase chain reaction (PCR-RFLP) method for the determination of erythrocytes CR1 genomic density polymorphism. Results: (1) compared with the control group, patients with chronic urticaria group of red blood cell CD35, CD55, CD58 expression was significantly increased (P0.05), CD44, CD59 the expression was significantly decreased (P0.05). (2) CR1 gene HH in chronic urticaria group, HL and LL genotype frequencies were 74.2%, 21.5% and 4.3% in control group, HH, HL and LL genotype frequencies were 71.8%, 23.6% and 4.6%, the difference distribution of CR1 genotype frequency in the two groups had no significant (P0.05). Conclusion: (1) the number of red blood cells in patients with chronic urticaria CD35 molecules than in the normal population increased, suggesting that the red cell immune function in patients with chronic urticaria are hyperfunction and disorder. Increase in the number of red blood cell CD35 molecule may be chronic urticaria natural immune response ability of hyperfunction and disorder (2) chronic urticaria. The surface of red blood cells decreased expression of CD44, CD55, CD58, CD59 expression increased, may have an effect on the incidence of chronic urticaria. (3) do not close genetic relationship between CR1 gene density changes associated with the number of red blood cells CD35 in patients with chronic urticaria, can be acquired by influencing factors.

【学位授予单位】：右江民族医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R758.24

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