成纤维细胞激活蛋白，整合素β1在皮肤鳞状细胞癌中的表达

发布时间：2018-03-21 04:46

本文选题：皮肤鳞状细胞癌　切入点：成纤维细胞激活蛋白　出处：《郑州大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的本研究检测成纤维细胞激活蛋白和整合素β1在皮肤鳞状细胞癌组织切片和正常皮肤切片中的表达情况,比较两种蛋白表达与皮肤鳞状细胞癌病理特征的关系,分析其表达之间的相关性,初步探讨成纤维细胞激活蛋白和整合素β1在皮肤鳞状细胞癌发生发展中的作用。材料和方法收集郑州大学第一附属医院整形外科自2006年～2008年确诊存档的皮肤鳞状细胞癌石蜡固定包埋的46例病理标本。病变部位来自头皮、面部、背部、四肢。所有患者术前均有详细的病例记录,未接受放射,激光,免疫等抗肿瘤治疗。男性20例,女性26例,年龄26～92岁(平均55.2岁)。皮肤鳞状细胞癌病理学分级：高分化、中分化、低分化。20例正常对照皮肤取自整形外科全厚皮片移植剩余皮片和美容手术切除皮肤。采用免疫组织化学方法进行实验。应用SPSS13.0统计软件进行统计学分析,P0.05为有统计学意义。结果 1.成纤维细胞激活蛋白在正常皮肤组织中无明显阳性表达。在皮肤鳞状细胞癌中成纤维细胞激活蛋白可见胞膜或胞质内呈明显棕黄色颗粒,阳性反应产物主要分布于肿瘤间质成纤维细胞的胞膜或胞质内。阳性表达率为87%。高分化鳞癌与中分化鳞癌比较差异无统计学意义(P0.05),中分化鳞癌与低分化鳞癌(P0.05),高分化鳞癌与低分化鳞癌比较差异有统计学意义(P0.05) 2.整合素β1在正常皮肤组织中呈现表面均质性高表达,阳性染色主要定位在细胞膜表面,胞质有少量表达。在皮肤鳞状细胞癌的细胞膜中,肿瘤细胞及肿瘤间质细胞中均有表达。阳性表达率为52%。在高分化鳞癌中呈高表达或接近高表达,在中低分化鳞癌细胞及间质中表达降低甚至消失。高中分化鳞癌比较差异有统计学意义(P0.05),高低分化鳞癌比较差异有统计学意义(P0.05),中低分化鳞癌比较无统计学意义(P0.05) 3.成纤维细胞激活蛋白的阳性表达与整合素β1的异常表达有关。二者的表达差异有统计学意义(P0.05)。随成纤维细胞激活蛋白的表达加强,整合素β1表达减弱。结论 1.成纤维细胞激活蛋白在皮肤鳞状细胞癌中表达而在正常皮肤中不表达,且成纤维细胞激活蛋白随皮肤鳞状细胞癌恶性程度的增高表达增强,提示成纤维细胞激活蛋白表达水平与肿瘤的恶性程度相关。 2.整合素β1在正常皮肤中呈均质表达,在皮肤鳞状细胞癌中表达率较正常皮肤阳性率降低,且整合素β1随皮肤鳞状细胞癌恶性程度的增高表达减弱。提示整合素β1的表达与肿瘤恶性程度呈负相关。 3.成纤维细胞激活蛋白表达与整合素β1表达异常,呈负相关,提示成纤维细胞激活蛋白的过表达可伴有整合素β1表达减弱,提示两者可能有共同的调节机制。 4.研究成纤维细胞激活蛋白与整合素β1在皮肤鳞状细胞癌间质中的表达,有望使其成为皮肤鳞状细胞癌治疗的新靶点。
[Abstract]:Objective to investigate the expression of fibroblast activator protein (FAPP) and integrin 尾 1 (integrin 尾 1) in skin squamous cell carcinoma (SCC) tissues and normal skin sections, and to compare the relationship between the expression of fibroblast activator protein (FAPP) and the pathological features of SCC. To explore the role of fibroblast activator protein (FAPP) and integrin 尾 1 (integrin 尾 1) in the development of cutaneous squamous cell carcinoma (SCC). Materials and methods. A total of 46 paraffin embedded specimens of squamous cell carcinoma of skin were collected from plastic surgery department of the first affiliated Hospital of Zhengzhou University from 2006 to 2008. The lesions were located on the scalp, face and back. Extremities. All patients had detailed records of cases before operation and had not received anti-tumor therapy such as radiation, laser, immunity, etc. Male 20 cases, female 26 cases, age 2692 years (mean 55.2 years old). Pathological grade of cutaneous squamous cell carcinoma: highly differentiated. Moderate differentiation, Low differentiation. 20 cases of normal control skin were obtained from the residual skin grafts of plastic surgery and cosmetic surgery. Immunohistochemical method was used to carry out the experiment. Statistical analysis using SPSS13.0 statistical software was statistically significant. Results. 1.Fibroblast activator protein was not expressed in normal skin tissue, fibroblast activator protein could be seen in the membrane or cytoplasm of skin squamous cell carcinoma. The positive reaction products were mainly distributed in the cell membrane or cytoplasm of the tumor interstitial fibroblasts. The positive expression rate was 87%. There was no significant difference between well-differentiated squamous cell carcinoma and moderately differentiated squamous cell carcinoma (P0.05), and there was no significant difference between moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma (P0.05). The difference between differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma was statistically significant (P0.05). 2. Integrin 尾 1 was highly expressed on the surface of normal skin tissues. The positive staining was mainly located on the surface of the cell membrane, and a small amount of expression was found in the cytoplasm, and in the cell membrane of squamous cell carcinoma of skin, the expression of integrin 尾 1 was higher than that of the normal skin. Both tumor cells and stromal cells were expressed. The positive expression rate was 52%. There was high expression or close to high expression in highly differentiated squamous cell carcinoma (SCC). The expression decreased or even disappeared in middle and low differentiated squamous cell carcinoma cells and stroma. There was significant difference in high and middle differentiated squamous cell carcinoma (P 0.05), high differentiation squamous cell carcinoma (P 0.05), and middle and low differentiation squamous cell carcinoma (P 0.05), but there was no significant difference in middle and low differentiation squamous cell carcinoma (P 0.05), but there was no significant difference between middle and low differentiation squamous cell carcinoma (P 0.05). 3. The positive expression of fibroblast activator protein was related to the abnormal expression of integrin 尾 1. There was a significant difference between the expression of integrin 尾 1 and the expression of integrin 尾 1. The expression of integrin 尾 1 decreased with the increase of expression of fibroblast activator protein. Conclusion. 1. Fibroblast activator protein was expressed in cutaneous squamous cell carcinoma but not in normal skin, and the expression of fibroblast activator protein increased with the malignant degree of skin squamous cell carcinoma. The results suggest that the expression level of fibroblast activator protein is related to the malignancy of tumor. 2. The expression of integrin 尾 1 in normal skin was homogeneous, and the positive rate of integrin 尾 1 in skin squamous cell carcinoma was lower than that in normal skin. The expression of integrin 尾 1 decreased with the increase of malignant degree of cutaneous squamous cell carcinoma, suggesting that the expression of integrin 尾 1 was negatively correlated with the degree of malignancy of the tumor. 3. The expression of fibroblast activator protein was negatively correlated with the abnormal expression of integrin 尾 _ 1, suggesting that the overexpression of fibroblast activator protein may be accompanied by a decrease of integrin 尾 _ 1 expression, suggesting that there may be a common regulatory mechanism between them. 4. To study the expression of fibroblast activator protein and integrin 尾 1 in the interstitial tissue of cutaneous squamous cell carcinoma, which is expected to become a new target for the treatment of cutaneous squamous cell carcinoma.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5

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