强脉冲光照射对体内外胶原蛋白合成的影响

发布时间：2018-04-04 17:00

  本文选题：强脉冲光　切入点：胶原　出处：《中南大学》2010年硕士论文

【摘要】： 目的 探讨强脉冲光(IPL)对小鼠皮肤胶原含量及人皮肤成纤维细胞胶原分泌的影响,部分阐明强脉冲光作用的分子生物学机制。 方法 1.采用能量密度为32J/cm2强脉冲光照射小鼠背部皮肤,左侧为对照,右侧采用强脉冲光照射,分别于照射后1天、7天、14天、28天切取皮肤样本,行HE染色,苦味酸-酸性品红法(V-G染色)观察皮肤组织及胶原变化。 2.采用能量密度为10、18、27、36、36J/cm2×2强脉冲光照射体外培养的人皮肤成纤维细胞,免疫细胞化学法检测Ⅰ、Ⅲ型胶原蛋白的变化。 结果 1.32J/cm2的强脉冲光照射对真皮层厚度的影响不显著(P0.05),但真皮胶原含量较对照组显著增加(P0.05)。表皮层未见显著改变。 2.不同能量密度强脉冲光照射人皮肤成纤维细胞并不能明显促进Ⅰ、Ⅲ型胶原分泌的增加(P0.05)。 结论 强脉冲光可促进小鼠真皮胶原含量增加,但不能显著刺激体外培养的人皮肤成纤维细胞Ⅰ、Ⅲ型胶原分泌的增加。
[Abstract]:PurposeTo investigate the effects of intense pulsed light (IPL) on the content of collagen in mouse skin and the secretion of collagen in human skin fibroblasts, the molecular biological mechanism of intense pulsed light was elucidated.Method1.The back skin of mice was irradiated with 32J/cm2 intense pulse light with energy density, the left side was used as control, and the right side was irradiated with intense pulse light. The skin samples were taken at 1 day, 7 days, 14 days and 28 days after irradiation, and the skin samples were stained with HE.The changes of skin tissue and collagen were observed by picric acid-acid fuchsin method (V-G staining).2.Human skin fibroblasts were irradiated with an energy density of 10 ~ (18) ~ (18) ~ (27) ~ (36) ~ (36) J / cm ~ (2) ~ (2). The changes of collagen type 鈪，

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