肉桂醛对黑素瘤A375细胞侵袭能力的影响

发布时间：2018-04-06 21:05

本文选题：肉桂醛　切入点：黑素瘤细胞　出处：《中国皮肤性病学杂志》2014年07期

【摘要】：目的探讨肉桂醛对体外二维和三维培养的人黑素瘤细胞株A375侵袭能力的影响。方法 A375细胞在体外二维培养后,在培养基中加入不同浓度(0,10,20和40μmol/L)肉桂醛,24h后细胞划痕实验检测细胞迁移,Transwell实验检测细胞侵袭情况。在组织工程皮肤基础上加入A375细胞,培养第5天开始在培养基中加入肉桂醛20μmol/L。在培养第15天固定组织工程皮肤标本,HE染色观察A375细胞生长和侵袭情况,免疫组织化学观察PCNA和MMP-9表达情况。二维和三维培养的A375细胞经肉桂醛处理后,ELISA法检测NF-κB活化情况。结果肉桂醛在二维培养条件下能抑制A375细胞的迁移,也能抑制A375细胞的侵袭,抑制强度和肉桂醛浓度成正比,各浓度试剂作用差异具有统计学意义(P均0.05)。肉桂醛能抑制三维培养模型内黑素瘤A375细胞的增殖和侵袭,肉桂醛处理前后黑素瘤细胞PCNA和MMP-9的表达差异也具有统计学意义(P均0.01)。肉桂醛能抑制二维和三维培养的A375细胞的NF-κB活化,各浓度间作用差异具有统计学意义(P均0.05)。结论肉桂醛能显著抑制体外二维和三维培养模型中黑素瘤细胞的侵袭。抑制NF-κB活化可能是其作用机制之一。
[Abstract]:Objective to investigate the effect of cinnamaldehyde on invasion of human melanoma cell line A375 in vitro.Methods A375 cells were cultured in two dimensional culture in vitro. The cell migration was detected by cell scratch assay and cell invasion was detected by Transwell assay with different concentrations of cinnamaldehyde (20 and 40 渭 mol / L) in culture medium.A375 cells were added on the basis of tissue engineering skin. On the 5th day of culture, cinnamaldehyde was added to the culture medium with 20 渭 mol / L cinnamaldehyde.The growth and invasion of A375 cells were observed by HE staining and the expression of PCNA and MMP-9 were observed by immunohistochemistry.The activation of NF- 魏 B in cultured A375 cells was detected by Elisa after treated with cinnamaldehyde.Results cinnamaldehyde could inhibit the migration of A375 cells and the invasion of A375 cells in two dimensional culture. The inhibition intensity was proportional to the concentration of cinnamaldehyde.Cinnamaldehyde could inhibit the proliferation and invasion of melanoma A375 cells in three dimensional culture model. The expression of PCNA and MMP-9 in melanoma cells was significantly different before and after treatment with cinnamaldehyde (P < 0.01).Cinnamaldehyde could inhibit the activation of NF- 魏 B in A375 cells cultured in two and three dimensions.Conclusion Cinnamaldehyde can significantly inhibit the invasion of melanoma cells in two-and three-dimensional culture models in vitro.Inhibition of NF- 魏 B activation may be one of its mechanisms.
【作者单位】：广州军区武汉总医院皮肤科;第三军医大学大坪医院野战外科研究所皮肤科;
【基金】：国家自然科学基金面上项目(81172211)
【分类号】：R739.5

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