AKT反馈激活拮抗4EGI-1对黑素瘤A375细胞的抑制作用

发布时间：2018-04-09 12:31

本文选题：黑素瘤　切入点：A细胞　出处：《中国肿瘤生物治疗杂志》2015年04期

【摘要】：目的:探索4EGI-1对黑素瘤A375细胞中AKT通路的激活作用及后者对4EGI-1抑制A375细胞增殖的影响。方法:用20、40、60μmol/L的4EGI-1处理黑素瘤A375细胞12、24 h,Western boltting检测4EGI-1对A375细胞中AKT磷酸化的影响;A375细胞分别受4EGI-1(40μmol/L)、BEZ235(5μmol/L)、4EGI-1(40μmol/L)+BEZ235(5μmol/L)联合处理,CCK-8实验和流式细胞术分别检测4EGI-1、BEZ235单独或联合处理对A375细胞增殖和细胞周期的影响,Western boltting检测对细胞周期相关蛋白Cyclin D1表达的影响。结果:4EGI-1促进A375细胞中AKT的磷酸化,且具有剂量依赖性;而BEZ235能够拮抗4EGI-1对AKT的促磷酸化作用。与4EGI-1组和BEZ235组相比,联合组的A375细胞增殖抑制率显著升高[24 h时,4EGI-1组和BEZ235组抑制率分别为(7.6±1.2)%和(2.4±3.1)%,而联合组抑制率为(19.8±4.3)%;P0.05],S期细胞比例显著降低(4EGI-1组和BEZ235组S期细胞比例分别为25.65%和25.82%,联合处理组细胞S期为13.08%;P0.05),Cyclin D1表达显著降低(4EGI-1组和BEZ235组Cyclin D1相对表达值为0.81±0.04和0.76±0.04,联合处理组细胞Cyclin D1相对表达值为0.25±0.03;P0.05)。结论:AKT反馈激活拮抗4EGI-1对黑素瘤A375细胞的抑制作用,联合使用AKT抑制药物能够增强4EGI-1对A375细胞的生长抑制作用。
[Abstract]:Aim: to investigate the effect of 4EGI-1 on the activation of AKT pathway in A375 cells and the effect of 4EGI-1 on the proliferation of A375 cells.Effects of proliferation and cell cycle on the expression of cell cycle associated protein Cyclin D1 by Western boltting.Results: the AKT phosphorylation of A375 cells was enhanced by 1: 4EGI-1 in a dose-dependent manner, while BEZ235 antagonized the phosphorylation of AKT by 4EGI-1.Compared with the 4EGI-1 group and the BEZ235 group,The expression of Cyclin D1 in the S phase of the treated group was 13.08. The relative expression of Cyclin D1 in the EGI-1 group and the BEZ235 group was 0.81 卤0.04 and 0.76 卤0.04, and the relative expression value of Cyclin D1 in the combined treatment group was 0.25 卤0.03p0.05.Conclusion the feedback activation of 4EGI-1 antagonizes the inhibitory effect of 4EGI-1 on A375 cells, and the combination of AKT inhibitor can enhance the growth inhibition of A375 cells induced by 4EGI-1.
【作者单位】：贵阳医学院附属医院皮肤科;
【基金】：贵州省中药现代化专项项目资助(No.20125018)~~
【分类号】：R739.5

【共引文献】